Illumina DNA Prep with Enrichment
广泛适用于各种应用的快速、集成的工作流程，能为定制panel、固定基因集的panel和全外显子组富集提供靶向重测序。
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Illumina DNA Prep with Enrichment是Illumina文库制备产品线中最快速、灵活的DNA靶向测序解决方案：
Illumina DNA Prep with Enrichment采用了快速且用户友好型的工作流程。On-Bead Tagmentation测序化学技术结合了简化的单一杂交实验方案，能将整个工作流程缩短至约6.5小时。在6.5小时之内即可完成从DNA的加入和提取到文库归一化、富集和富集后扩增等一系列过程。全部手动操作时间减少至约2小时。
Illumina DNA Prep with Enrichment支持的DNA上样量范围（50-1000 ng）很广，并且支持包括血液、唾液、基因组DNA和福尔马林固定石蜡包埋（FFPE）组织在内的多种样本类型。直接将血液和唾液加入Illumina DNA Prep酶法片段化反应，能减少所需的定量步骤，节约了时间和金钱。
在满足不同研究需求的同时，Illumina DNA Prep with Enrichment解决方案可提供大小一致的插入片段，具备均一的覆盖度和优化的性能，不论DNA上样量如何。微珠技术最大限度地减少了偏差和出错，使得所有Illumina测序系统都能获得高重现性的数据。
通过这种互动体验，感受Illumina文库制备酶切法片段化技术为您的实验室带来的力量。我们的文库制备解决方案支持各种应用中的全基因组测序、灵活兼容Illumina测序仪，整合血液和唾液的样本制备，且无需定量。
查看信息图
| 仪器 | 推荐的样本数 | 读长 |
|---|---|---|
| iSeq 100 System | TruSight Cancer: 4 samples per run (based on 280x mean targeted coverage; 99% target coverage at 20x) TruSight Cardio: 4 samples per run (based on 170x mean targeted coverage; 100% target coverage at 20x) |
2 x 100 bp 2 x 100 bp |
| MiniSeq System | TruSight Cancer (samples per run): mid output: 8, high output: 25 (based on 280x mean targeted coverage; 99% target coverage at 20x) TruSight Cardio (samples per run): mid output: 8, high output: 25 (based on 170x mean targeted coverage; 100% target coverage at 20x) |
2 x 100 bp 2 x 100 bp |
| MiSeq System | TruSight Cancer (samples per run): v2 reagents: 15, v3 reagents: 25 (based on 280x mean targeted coverage; 99% target coverage at 20x) TruSight Cardio (samples per run): v2 reagents: 15, v3 reagents: 25 (based on 170x mean targeted coverage; 100% target coverage at 20x) TruSight One (samples per run): v2 reagents: 1, v3 reagents: 3 (based on 120x mean targeted coverage; 95% target coverage at 20x) |
2 x 100 bp 2 x 100 bp 2 x 100 bp (up to 2 x 150 bp is possible) |
| NextSeq 550 System | Exome (samples per run): mid output: 5, high output: 16 (based on 50x mean targeted coverage; 90% target coverage at 20X) TruSight One (samples per run): mid output: 12, high output: 36 (based on 120x mean targeted coverage; 95% target coverage at 20x) TruSight One Expanded (samples per run): mid output: 7, high output: 24 (based on 130x mean targeted coverage; 97% target coverage at 20x) |
2 x 100 bp 2 x 100 bp (up to 2 x 150 bp is possible) 2 x 100 bp (up to 2 x 150 bp is possible) |
| HiSeq 2500 System | Exome (samples per run, single flow cell): rapid run: 12, high output with v4 reagents: 80 (based on 50x mean targeted coverage; 90% target coverage at 20X) TruSight One (samples per run, single flow cell): rapid run: 27, high output with v4 reagents: 184 (based on 120x mean targeted coverage; 95% target coverage at 20x) TruSight One Expanded (samples per run, single flow cell): rapid run: 18, high output with v4 reagents: 121 (based on 130x mean targeted coverage; 97% target coverage at 20x) |
2 x 100 bp 2 x 100 bp (up to 2 x 150 bp is possible) 2 x 100 bp |
| HiSeq 4000 System | TruSight One Expanded (samples per run, single flow cell): 151 (based on 130x mean targeted coverage; 97% target coverage at 20x) | 2 x 100 bp |
| NovaSeq 6000 System | Exome (samples per run, by flow cell type): S1: 128, S2: 164, S4: 400 (based on 50x mean targeted coverage; 90% target coverage at 20X; S4 limited by available indexes) | 2 x 100 bp |
Tumor exome sequencing with Illumina DNA Prep with Enrichment and the DRAGEN Platform
Application Note | PDF 1 MB
Somatic Variant Detection in FFPE Samples with Nextera Flex for Enrichment
Application Note | HTML
Whole-Exome Sequencing with Nextera Flex for Enrichment
Application Note | HTML
Fast, Flexible, and Self-Normalizing Library Prep with Nextera Flex Chemistry
Application Note | HTML
Data Sheet | HTML | 7 versions
Illumina library preparation solutions
Brochure | PDF 2 MB
NextSeq 550 exome sequencing solution
Application Note | PDF < 1 MB
NextSeq 1000 and NextSeq 2000 exome sequencing solution
Application Note | PDF < 1 MB
User-definable parameters in the Illumina DNA Prep with Enrichment workflow
Technical Note | PDF < 1 MB
Data Sheet | PDF | 5 versions
Urinary Pathogen ID/AMR Panel Reference Documentation
Illumina Adapter Sequences Documentation
Illumina DNA Prep with Enrichment Consumables & Equipment Documentation
Illumina DNA Prep with Enrichment Reference Guide Documentation
Illumina DNA Prep with Enrichment Checklist Documentation
Index Adapters Pooling Guide Documentation
Enrichment BaseSpace App Documentation
Illumina DNA Prep with Enrichment Documentation
自定义协议选择器
Generates customized, end-to-end instructions
*Results from example data set. Actual performance may vary.
