Nextera Flex for Enrichment
广泛适用于各种应用的快速、集成的工作流程，能为定制panel、固定基因集的panel和全外显子组富集提供靶向重测序。Read More...
Nextera Flex for Enrichment是Illumina文库制备产品线中最快速、灵活的DNA靶向测序解决方案：
Nextera Flex for Enrichment采用了快速且用户友好型的工作流程。On-Bead Tagmentation测序化学技术结合了简化的单一杂交实验方案，能将整个工作流程缩短至约6.5小时。在6.5小时之内即可完成从DNA的加入和提取到文库归一化、富集和富集后扩增等一系列过程。全部手动操作时间减少至约2小时。
Nextera Flex for Enrichment支持的DNA上样量范围（50-1000 ng）很广，并且支持包括血液、唾液、基因组DNA和福尔马林固定石蜡包埋（FFPE）组织在内的多种样本类型。直接将血液和唾液加入Nextera DNA Flex酶法片段化反应，能减少所需的定量步骤，节约了时间和金钱。
在满足不同研究需求的同时，Nextera Flex for Enrichment解决方案可提供大小一致的插入片段，具备均一的覆盖度和优化的性能，不论DNA上样量如何。微珠技术最大限度地减少了偏差和出错，使得所有Illumina测序系统都能获得高重现性的数据。
通过这种互动体验，感受Nextera技术为您的实验室带来的力量。Nextera文库制备试剂盒解决方案支持各种应用中的全基因组测序、灵活兼容Illumina测序仪，整合血液和唾液的样本制备，且无需定量。
查看信息图
| 仪器 | 推荐的样本数 | 读长 |
|---|---|---|
| iSeq 100 System | TruSight Cancer: 4 samples per run (based on 280x mean targeted
coverage; 99% target coverage at 20x) TruSight Cardio: 4 samples per run (based on 170x mean targeted coverage; 100% target coverage at 20x) |
2 x 100 bp 2 x 100 bp |
| MiniSeq System | TruSight Cancer (samples per run): mid output: 8, high output: 25 (based
on 280x mean targeted coverage; 99% target coverage at 20x) TruSight Cardio (samples per run): mid output: 8, high output: 25 (based on 170x mean targeted coverage; 100% target coverage at 20x) |
2 x 100 bp 2 x 100 bp |
| MiSeq System | TruSight Cancer (samples per run): v2 reagents: 15, v3 reagents: 25
(based on 280x mean targeted coverage; 99% target coverage at 20x) TruSight Cardio (samples per run): v2 reagents: 15, v3 reagents: 25 (based on 170x mean targeted coverage; 100% target coverage at 20x) TruSight One (samples per run): v2 reagents: 1, v3 reagents: 3 (based on 120x mean targeted coverage; 95% target coverage at 20x) |
2 x 100 bp 2 x 100 bp 2 x 100 bp (up to 2 x 150 bp is possible) |
| NextSeq 550 System | Exome (samples per run): mid output: 5, high output: 16 (based on 50x
mean targeted coverage; 90% target coverage at 20X) TruSight One (samples per run): mid output: 12, high output: 36 (based on 120x mean targeted coverage; 95% target coverage at 20x) TruSight One Expanded (samples per run): mid output: 7, high output: 24 (based on 130x mean targeted coverage; 97% target coverage at 20x) |
2 x 100 bp 2 x 100 bp (up to 2 x 150 bp is possible) 2 x 100 bp (up to 2 x 150 bp is possible) |
| HiSeq 2500 System | Exome (samples per run, single flow cell): rapid run: 12, high output
with v4 reagents: 80 (based on 50x mean targeted coverage; 90% target
coverage at 20X) TruSight One (samples per run, single flow cell): rapid run: 27, high output with v4 reagents: 184 (based on 120x mean targeted coverage; 95% target coverage at 20x) TruSight One Expanded (samples per run, single flow cell): rapid run: 18, high output with v4 reagents: 121 (based on 130x mean targeted coverage; 97% target coverage at 20x) |
2 x 100 bp 2 x 100 bp (up to 2 x 150 bp is possible) 2 x 100 bp |
| HiSeq 4000 System | TruSight One Expanded (samples per run, single flow cell): 151 (based on 130x mean targeted coverage; 97% target coverage at 20x) | 2 x 100 bp |
| NovaSeq 6000 System | Exome (samples per run, by flow cell type): S1: 128, S2: 164, S4: 400 (based on 50x mean targeted coverage; 90% target coverage at 20X; S4 limited by available indexes) | 2 x 100 bp |
Tumor exome sequencing with Illumina DNA Prep with Enrichment and the DRAGEN Platform
Application Note | PDF1 MB
Somatic Variant Detection in FFPE Samples with Nextera Flex for Enrichment
Application Note | HTML
Whole-Exome Sequencing with Nextera Flex for Enrichment
Application Note | HTML
Fast, Flexible, and Self-Normalizing Library Prep with Nextera Flex Chemistry
Application Note | HTML
NextSeq 550 exome sequencing solution
Application Note | PDF< 1 MB
Data Sheet | PDF | 5 versions
Enrichment BaseSpace App Documentation
Illumina Adapter Sequences Document Documentation
Index Adapters Pooling Guide Documentation
Illumina DNA Prep with Enrichment Consumables & Equipment Documentation
Illumina DNA Prep with Enrichment Reference Guide Documentation
Illumina DNA Prep with Enrichment Checklist Documentation
Illumina DNA Prep with Enrichment Documentation
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