Nextera Flex for Enrichment

广泛适用于各种应用的快速、集成的工作流程,能为定制panel、固定基因集的panel和全外显子组富集提供靶向重测序。Read More...
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Nextera DNA Flex Pre-Enrichment文库制备和富集试剂——96个样本(8次反应,12重富集)

20025524

Nextera DNA Flex Pre-Enrichment文库制备和富集试剂——16个样本(16次反应,1重富集)

20025523

Nextera DNA Flex Pre-Enrichment文库制备试剂(96样本)

20025520

Nextera DNA Flex Pre-Enrichment文库制备试剂(16样本)

20025519

Panel

Illumina Exome Panel——仅含富集寡核苷酸(8或12次富集反应)

20020183

TruSight Cancer——仅含富集寡核苷酸(4或8次富集反应)

FC-121-0202

TruSight One——仅含富集寡核苷酸(6次富集反应)

20029227

TruSight One Expanded——仅含富集寡核苷酸(6次富集反应)

20029226

TruSight Cardio——仅含富集寡核苷酸(8次富集反应)

20029229

TruSeq Neurodegeneration——仅含富集寡核苷酸(8次富集反应)

20029550

Illumina Custom Enrichment Panel(8次富集反应)

20025371


Index Adapters

IDT® for Illumina Nextera DNA Unique Dual Indexes Set A(96标签,96样本)

20027213

IDT® for Illumina Nextera DNA Unique Dual Indexes Set B(96标签,96样本)

20027214

IDT® for Illumina Nextera DNA Unique Dual Indexes Set C(96标签,96样本)

20027215

IDT® for Illumina Nextera DNA Unique Dual Indexes Set D(96标签,96样本)

20027216

IDT® for Illumina Nextera DNA Unique Dual Indexes Sets A-D(384标签,384样本)

20027217

配件产品

Flex Lysis Reagent Kit(96次反应)

20018706

Infinium FFPE QC Kit(384个反应)

WG-321-1001

Infinium MIDI Heatblock Insert

BD-60-601

Illumina游离接头阻断试剂(12次反应)

20024144

Illumina游离接头阻断试剂(48次反应)

20024145

Product Highlights

Nextera Flex for Enrichment是Illumina文库制备产品线中最快速、灵活的DNA靶向测序解决方案:

  • 比标准Illumina文库制备和富集快85%的省时解决方案
  • 用于血液和唾液的集成DNA提取方案
  • 采用了基于微珠的文库制备技术,性能更佳,结果可靠
快速的文库制备和富集工作流程

Nextera Flex for Enrichment采用了快速且用户友好型的工作流程。On-Bead Tagmentation测序化学技术结合了简化的单一杂交实验方案,能将整个工作流程缩短至约6.5小时。在6.5小时之内即可完成从DNA的加入和提取到文库归一化、富集和富集后扩增等一系列过程。全部手动操作时间减少至约2小时。

整合的DNA提取

Nextera Flex for Enrichment支持的DNA上样量范围(50-1000 ng)很广,并且支持包括血液、唾液、基因组DNA和福尔马林固定石蜡包埋(FFPE)组织在内的多种样本类型。直接将血液和唾液加入Nextera DNA Flex酶法片段化反应,能减少所需的定量步骤,节约了时间和金钱。

经过优化的性能

在满足不同研究需求的同时,Nextera Flex for Enrichment解决方案可提供大小一致的插入片段,具备均一的覆盖度和优化的性能,不论DNA上样量如何。微珠技术最大限度地减少了偏差和出错,使得所有Illumina测序系统都能获得高重现性的数据。

探索文库制备的新时代

通过这种互动体验,感受Nextera技术为您的实验室带来的力量。Nextera文库制备试剂盒解决方案支持各种应用中的全基因组测序、灵活兼容Illumina测序仪,整合血液和唾液的样本制备,且无需定量。

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Nextera DNA Flex Library Prep Kit简介

Frequently Purchased Together

规格

计划推荐

仪器 推荐的样本数 读长
iSeq 100 System TruSight Cancer: 4 samples per run (based on 280x mean targeted coverage; 99% target coverage at 20x)
TruSight Cardio: 4 samples per run (based on 170x mean targeted coverage; 100% target coverage at 20x)
2 x 100 bp

2 x 100 bp
MiniSeq System TruSight Cancer (samples per run): mid output: 8, high output: 25 (based on 280x mean targeted coverage; 99% target coverage at 20x)
TruSight Cardio (samples per run): mid output: 8, high output: 25 (based on 170x mean targeted coverage; 100% target coverage at 20x)
2 x 100 bp

2 x 100 bp
MiSeq System TruSight Cancer (samples per run): v2 reagents: 15, v3 reagents: 25 (based on 280x mean targeted coverage; 99% target coverage at 20x)
TruSight Cardio (samples per run): v2 reagents: 15, v3 reagents: 25 (based on 170x mean targeted coverage; 100% target coverage at 20x)
TruSight One (samples per run): v2 reagents: 1, v3 reagents: 3 (based on 120x mean targeted coverage; 95% target coverage at 20x)
2 x 100 bp

2 x 100 bp

2 x 100 bp (up to 2 x 150 bp is possible)
NextSeq 550 System Exome (samples per run): mid output: 5, high output: 16 (based on 50x mean targeted coverage; 90% target coverage at 20X)
TruSight One (samples per run): mid output: 12, high output: 36 (based on 120x mean targeted coverage; 95% target coverage at 20x)
TruSight One Expanded (samples per run): mid output: 7, high output: 24 (based on 130x mean targeted coverage; 97% target coverage at 20x)
2 x 100 bp

2 x 100 bp (up to 2 x 150 bp is possible)
2 x 100 bp (up to 2 x 150 bp is possible)
HiSeq 2500 System Exome (samples per run, single flow cell): rapid run: 12, high output with v4 reagents: 80 (based on 50x mean targeted coverage; 90% target coverage at 20X)
TruSight One (samples per run, single flow cell): rapid run: 27, high output with v4 reagents: 184 (based on 120x mean targeted coverage; 95% target coverage at 20x)
TruSight One Expanded (samples per run, single flow cell): rapid run: 18, high output with v4 reagents: 121 (based on 130x mean targeted coverage; 97% target coverage at 20x)
2 x 100 bp

2 x 100 bp (up to 2 x 150 bp is possible)
2 x 100 bp
HiSeq 4000 System TruSight One Expanded (samples per run, single flow cell): 151 (based on 130x mean targeted coverage; 97% target coverage at 20x) 2 x 100 bp
NovaSeq 6000 System Exome (samples per run, by flow cell type): S1: 128, S2: 164, S4: 400 (based on 50x mean targeted coverage; 90% target coverage at 20X; S4 limited by available indexes) 2 x 100 bp

具体的方法工作流程范例

 

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