鸟枪法宏基因组测序简介
鸟枪法宏基因组测序技术使研究人员能够对给定的复杂样本中存在的所有生物体的所有基因进行全面的检测。微生物学家可以利用该方法评估细菌多样性，并检测各种环境中微生物的丰度。鸟枪法宏基因组学还可以用来研究不可培养的微生物，其他方法很难或无法分析这些微生物。
与毛细管测序或基于PCR的方法不同，新一代测序（NGS）可以使研究人员同时对数千种生物进行测序。基于NGS的宏基因组测序能够在单一测序运行中组合多个样本，每个样本都可实现高测序覆盖度，因此可以检测微生物群落中丰度非常低的生物，而利用其他方法可能无法识别这些生物或者价格太高。
16S rRNA测序是宏基因组学研究的另一种方法。
IDbyDNA's Explify Platform Improves Results for Clinical Metagenomics Research
The Explify Platform is a fully supported NGS-based, clinical metagenomics technology suite for clinical laboratories. Watch this video for a virtual tour to understand how the Explify Platform can help your lab streamline processes, improve results, and much, much more.
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推荐的鸟枪法宏基因组学工作流程
有多种方法可以开展鸟枪法宏基因组测序，但工作流程的每个步骤都有一些推荐使用的产品。
点击下方的查看工作流程各步骤所使用的产品。
Nextera DNA Flex Library Prep Kit
一个快速、集成的工作流程，适合广泛的应用，从人类全基因组测序到扩增子、质粒和各种微生物。
Nextera XT Library Prep Kit90分钟内即可用小型基因组（细菌、古细菌、病毒）、扩增子和质粒制备出可直接用于测序的文库。
TruSeq DNA PCR-Free Library Preparation Kit
为全基因组测序应用提供简单全面的文库制备方案。研究人员可对多种生物进行测序，从细菌等小型基因组到人类全基因组。
One Codex
在BaseSpace Sequence Hub使用快速、全面、准确的数据平台快速评估样本，进行宏基因组分析
GENIUS宏基因组学: 立即获得结果使用CosmosID经审核的基因组数据库和高性能算法，在物种、亚种和菌株水平上进行快速、准确和可行的细菌鉴定。
Metagenomics and Infectious Diseases
Infectious Disease Surveillance
NGS supports effective strategies to track infectious disease transmission. Metagenomics can help scientists identify novel pathogens and study zoonotic reservoirs to help predict and prevent outbreaks.
Learn MoreCOVID-19 Host Risk and Response
Studies of host genetic variation and immune responses may reveal COVID-19 therapeutic targets. Metagenomics can help researchers analyze microbiome differences between individuals with severe or mild COVID-19.
Learn More
Metagenomic Sequencing of Environmental Samples
Soil Microbiome Profiling
This metagenomic shotgun sequencing workflow offers fully automated soil sample preparation, sequencing, and analysis, to help researchers characterize soil microbial communities.
Read Article对环境DNA进行鸟枪法测序
环境DNA（eDNA）测序是一种迅速兴起的方法，可研究生物多样性并监测生态系统的改变。环境DNA的鸟枪法测序有助于研究可能在样本中大量存在的物种，例如细菌或小型真核生物。
了解更多希望收到有关微生物基因组学的快讯、案例研究和信息？请输入您的电子邮箱地址。
更多资源
Clinical Metagenomics and COVID-19
Robert Schlaberg and Lauge Farnaes from IDbyDNA discuss applications of metagenomics to infectious diseases in the clinical realm.
Soil Metagenomics Workflow
Explore taxonomic and functional diversity of soil microbial communities with this comprehensive shotgun metagenomics workflow.
Metagenomics and Emerging Pathogens
IDbyDNA experts discuss use of metagenomics in facing the threat of emerging pathogens.
参考文献
- Baker BJ, Sheik CS, Taylor CA, Jain S, Bhasi A, et al.(2013) Community transcriptomic assembly reveals microbes that contribute to deep-sea carbon and nitrogen cycling.ISME J 10.1038/ismej.2013.85.
- McLean JS, Lombardo MJ, Ziegler MG, Novotny M, Yee-Greenbaum J, et al.(2013) Genome of the pathogen Porphyromonas gingivalis recovered from a biofilm in a hospital sink using a high-throughput single-cell genomics platform.Genome Res 23:867–77.
- Schofield MM, Sherman DM.(2013) Meta-omic characterization of prokaryotic gene clusters for natural product biosynthesis.Curr Opin Biotechnol: 10.1016/j.copbio.2013.05.001.
- Neafsey DE.(2013) Genome sequencing sheds light on emerging drug resistance in malaria parasites.Nat Genet 45:589–90.