Illumina DNA Prep Training
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Illumina DNA Prep为多个全基因组测序应用带来灵活性。
Learn more about the technology
Illumina DNA Prep采用一个快速且用户友好的工作流程。微珠tagmentation技术将文库制备的总时间（从DNA提取到文库归一化）缩短至3.5小时。
Illumina DNA Prepx的工作流程支持广泛的DNA起始量（1-500 ng），多种类型的样本，以及小型和大型基因组。这个流程包括从血液、唾液、干血斑*或细菌菌落*中提取DNA。灵活地对人类或其他复杂的大型基因组以及扩增子或微生物群落进行测序，这一切通过单个试剂盒都能实现。
灵活地对人类或其他复杂的大型基因组以及扩增子或微生物群落进行测序，这一切通过单个试剂盒都能实现。
在满足不同研究需求的同时，Nextera DNA Flex的工作流程带来大小一致的插入片段、均一的覆盖和优化的性能，不论DNA起始量或基因组大小如何。这种基于微珠的技术最大限度地减少了偏向性和错误机会，带来高度可重现的测序数据。
IDT® for Illumina®-Nextera™ DNA UD Indexes set A、B、C和D标签提供多达384个唯一双标签，可准确分配read，高效利用流动槽。 这些唯一双标签码使用10个碱基对码。碱基对标签码的这种改变需要调整测序运行设置。
Nextera DNA CD Indexes支持多达96个样本的组合双标签。24规格的CD标签以试管的形式提供，96规格的CD标签以孔板的形式提供
对于全基因组测序，推荐使用Illumina DNA Prep作为Nextera DNA Library Prep Kit（已停产）的替代产品。如果您之前在ATAC-Seq**或其他定制应用中使用Nextera DNA（货号FC-121-1030）或独立组件（货号15027865和15027866），则建议您使用Illumina Tagment DNA TDE1 Enzyme and Buffer Kit作为替代产品。
*提供经过验证的实验方案。
**客户报告的应用
通过这种互动体验，感受Nextera技术为您的实验室带来的力量。Nextera文库制备试剂盒解决方案支持各种应用中的全基因组测序、灵活兼容Illumina测序仪，整合血液和唾液的样本制备，且无需定量。
查看信息图
| 仪器 | 推荐的样本数 | 读长 |
|---|---|---|
| NextSeq 550 System | 1 sample per run (high output; based on 30× coverage of a human genome) | Up to 2 × 150 bp |
| NovaSeq 6000 System | 2–10 samples per run (dual flow cell; based on 30× coverage of a human genome) | Up to 2 × 125 bp (rapid run) Up to 2 × 150 bp (high output) |
| Illumina DNA Prep | TruSeq DNA PCR-Free | TruSeq DNA Nano | |
|---|---|---|---|
| Assay Time | ~3-4 hours (from DNA extraction to normalized library) | 5 hours total assay time | ~6 hours total assay time |
| Automation Capability | 液体处理机器人 | 液体处理机器人 | 液体处理机器人 |
| Description | A fast, flexible workflow for a wide range of research applications and sample types, from human to microbial whole-genome sequencing and more. | A simple, all-inclusive PCR-free prep for whole-genome sequencing studies with the ability to sequence through challenging regions of the genome. | A low-input research method that delivers high genome coverage quality and reduced bias. |
| Hands-On Time | 1-1.5 hours | 4 hours | ~4 hours |
| Input Quantity | Small genomes (e.g. microbial): 1-500 ng DNA. Large genomes (e.g. human): 100-500 ng DNA. (For blood and saliva, see the reference guide). | 1 ug DNA | 100 ng genomic DNA |
| Mechanism of Action | 连接磁珠的转座酶 | Mechanical DNA fragmentation and adapter ligation. Workflow is PCR-free and gel-free. | Mechanical DNA fragmentation and adapter ligation. Workflow uses reduced-bias PCR and is gel-free. |
| Method | Amplicon Sequencing, De Novo Sequencing, Shotgun Sequencing, Whole-Genome Sequencing | Genotyping by Sequencing, Shotgun Sequencing, Whole-Genome Sequencing | Genotyping by Sequencing, Shotgun Sequencing, Whole-Genome Sequencing |
| Multiplexing | Up to 384 unique dual (UD) combinations and 96 combinatorial dual (CD) combinations | Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations (available soon) | Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations (available soon) |
| Specialized Sample Types | Blood, Not FFPE-Validated, Saliva | Not FFPE-Compatible | Low-Input Samples, Not FFPE-Compatible |
| Species Category | Any Species, Drosophila, Mammalian, Nematode, Plant, Virus, Yeast, Zebrafish, 人类, 大鼠, 小鼠, 细菌 | Mammalian, Other, Plant, 人类, 大鼠, 小鼠 | Mammalian, Other, Plant, 人类, 大鼠, 小鼠 |
| Species Details | Compatible with any species | Compatible with most large DNA genomes. | Compatible with most large DNA genomes. |
| Target Insert Size | ~350bp | 350 bp or 550 bp | 350 bp or 550 bp |
(A) 固定有转座子酶的磁珠介导gDNA片段化与Illumina测序引物的添加同时发生。(B) 循环数较少的PCR扩增生成了可立即测序的DNA片段，并添加标签和接头。(C) 对可上机测序的片段进行洗脱并混合。
标题：The process begins with unfragmented DNA binding to the BLT. Since each transposome is only able to perform the reaction once, saturation of the BLT acts as a DNA normalization step, saving time from doing it at the end of the library prep. It also gives more even and uniform coverage across the region with little region-specific bias. Note that at least 100 ng of input DNA is required for integrated normalization
Application Note | PDF 3 MB
An automated Nextera DNA Flex library preparation workflow for high-throughput metagenomics
Application Note | PDF < 1 MB
Nextera Crude Lysate Protocol for Metagenomic Whole-Genome Sequencing Studies
Application Note | PDF < 1 MB
Human Whole-Genome Sequencing with the Nextera DNA Flex Library Preparation Kit
Application Note | PDF < 1 MB
Direct Bacterial Colony Sequencing with the Nextera™ DNA Flex Library Preparation Kit
Application Note | PDF 1 MB
Microbial Whole-Genome Sequencing with the Nextera DNA Flex Library Preparation Kit
Application Note | PDF < 1 MB
Fast, Flexible, and Self-Normalizing Library Prep with Nextera Flex Chemistry
Application Note | HTML
Nextera DNA Flex Library Preparation for Soil Shotgun Metagenomics Analysis
Application Note | HTML
Data Sheet | PDF | 5 versions
Data Sheet | PDF | 5 versions
Illumina library preparation solutions
Brochure | PDF 2 MB
Illumina DNA Prep Support Documentation
Illumina DNA Prep Consumables & Equipment List Documentation
Illumina DNA Prep Reference Guide Documentation
Trim Adapters for Nextera DNA Flex in Local Run Manager for MiniSeq Documentation
Index Adapters Pooling Guide Documentation
Illumina DNA Prep Checklist Documentation
自定义协议选择器
Generates customized, end-to-end instructions
Generate whole-genome sequencing libraries and efficiently interrogate samples with limited available DNA.
Simple, all-inclusive whole-genome sequencing (WGS) library preparation that provides accurate and comprehensive coverage of complex genomes.
