AmpliSeq for Illumina Cancer Hotspot Panel v2

研究小组有针对性地调查了50个已知的与癌症相关的基因。 Read More...
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Panel

AmpliSeq™ Cancer HotSpot Panel v2 for Illumina®

20019161


Library Prep

AmpliSeq™ Library PLUS (24 Reactions) for Illumina®

20019101

AmpliSeq™ Library PLUS (96 Reactions) for Illumina®

20019102

AmpliSeq™ Library PLUS (384 Reactions) for Illumina®

20019103


Index Adapters

AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)

20019104

AmpliSeq™ CD Indexes Set A for Illumina®

20019105

AmpliSeq™ CD Indexes Set B for Illumina®

20019106

AmpliSeq™ CD Indexes Set C for Illumina®

20019107

AmpliSeq™ CD Indexes Set D for Illumina®

20019167

AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)

20031676

配件产品

AmpliSeq™ for Illumina® Sample ID Panel

20019162

AmpliSeq™ for Illumina® Direct FFPE DNA

20023378

AmpliSeq™ Library Equalizer for Illumina®

20019171

Product Highlights

The AmpliSeq for Illumina Cancer Hotspot Panel v2 is a targeted resequencing assay for researching somatic mutations across the hotspot regions of 50 genes with known associations to cancer, as identified in the Catalogue of Somatic Mutations in Cancer (COSMIC)1 database. Key features include:

Relevant Gene Content
  • Target ~2800 COSMIC mutations from 50 oncogenes and tumor suppressor genes
Fast, Streamlined Workflow
  • Prepare sequencing-ready libraries in a single day from as little as 1 ng of high quality or 10 ng of formalin-fixed, paraffin-embedded (FFPE) samples
Accurate Data
  • Detect somatic mutations down to 5% variant allele frequency using local or cloud-based analysis

The Cancer HotSpot Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.

This panel enables highly sensitive variant detection within multiple cancer types, including lung, colon, breast, ovarian, melanoma, and prostate. The ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.

Learn More About AmpliSeq for Illumina

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产品比较

AmpliSeq for Illumina Cancer Hotspot Panel v2 AmpliSeq for Illumina Focus Panel AmpliSeq for Illumina Comprehensive Panel v3 TruSight Tumor 15
Assay Time 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) 5-6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) 5-6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) 7 hours
Cancer Type Solid Tumor, 泛癌种 Solid Tumor Solid Tumor Solid Tumor
Content Specifications Hotspot regions of 50 genes with known associations to cancer DNA and RNA targets for 52 oncogenes DNA and RNA targets for 161 oncogenes Amplifies 250 amplicons from 15 genes associated with solid tumors
Description Somatic analysis research into hotspot regions of 50 cancer-related genes. Somatic analysis research on 52 genes associated with solid tumor cancers. Somatic analysis research on hotspot and full-length targets of genes associated with solid tumor cancers. Focused panel to study relevant solid tumor somatic variants in 15 genes using a simple workflow.
Input Quantity 1–100 ng (10 ng recommended per pool) 1–100 ng (10 ng recommended per pool) 1–100 ng (10 ng recommended per pool) 20 ng
Method Amplicon Sequencing, 靶向DNA测序 Amplicon Sequencing, 靶向DNA测序, 靶向RNA测序 Amplicon Sequencing, 靶向DNA测序, 靶向RNA测序 Amplicon Sequencing, 靶向DNA测序
Multiplexing 96 dual index combinations 96 dual index combinations 96 dual index combinations 1-24-plex
Nucleic Acid Type DNA DNA, RNA DNA, RNA DNA
Specialized Sample Types FFPE Tissue FFPE Tissue FFPE Tissue FFPE Tissue
Species Category 人类 人类 人类 人类

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References
  1. Kingsmore S. Catalogue of Somatic Mutations in Cancer – Home Page. http://cancer.sanger.ac.uk/cosmic. Accessed October 25, 2017.