Order your MiniSeq reagents and library prep kits together for additional savings and convenience. Select item 20005610 and add to your cart.
TruSight肿瘤15使用下一代测序(NGS)技术，对实体肿瘤中常见的15个基因进行全面评估。它能准确检测20ng起始DNA的低频变异，并对福尔马林固定、石蜡包埋(FFPE)的肿瘤组织进行了优化。
具有快速的工作流程，可以很容易地集成到实验室程序中，该面板提供了一个准确、经济、快速的实体肿瘤分析的单一分析方法。
TruSight肿瘤面板提供:
使用TruSight Tumor 15制备3份人类参考样本和5份从肺、结肠、黑色素瘤和乳腺肿瘤中提取的ffpe DNA样本。这些文库在MiniSeq系统上测序，使用2 × 150 bp读取长度配置双索引的高输出试剂盒。Q30及以上的碱基产量为94.9%，总产量为7.6 g。
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| 仪器 | 推荐的样本数 | 读长 |
|---|---|---|
| MiniSeq System | Samples per run: mid output: 2-3, high output: 8 (based on 93.5% of the bases covered at 500x minimum) | 2 × 150 bp (max recommended) |
| MiSeq System | 8 samples per run with v3 reagents (based on 93.5% of the bases covered at 500x minimum) | 2 × 151 bp (max recommended) |
| AKT1 | GNA11 | NRAS |
| BRAF | GNAQ | PDGFRA |
| EGFR | KIT | PIK3CA |
| ERBB2 | KRAS | RET |
| FOXL2 | MET | TP53 |
Analysis of Cell-Free DNA with the TruSight Tumor 15 Assay
White Paper | PDF < 1 MB
Assessing the Quality of FFPE Samples for Preparation of TruSight® Tumor 15 Sequencing Libraries
White Paper | PDF < 1 MB
Analysis of TP53 Variants in Bone Marrow Aspirates With TruSight Tumor 15
White Paper | PDF < 1 MB
Data Sheet | PDF < 1 MB
Technical Note | PDF < 1 MB
TruSight Tumor 15 Data Analysis
Technical Note | PDF < 1 MB
TruSight Tumor 15 Reference Guide Documentation
TruSight Tumor 15 Checklist Documentation
MiSeq Reporter TruSight Tumor 15 Workflow Guide Documentation
Illumina Experiment Manager User Guide Documentation
TruSight Tumor 15 Consumables and Equipment Documentation
TruSight Tumor 15 Support Documentation
自定义协议选择器
Generates customized, end-to-end instructions
Kit requires MiSeq Software v2.6 or higher and the Illumina Annotation Engine. Download files
