RNA测序

RNA测序简介

RNA测序(RNA-Seq)正在彻底改变转录组的研究。它是一种高度灵敏和准确的检测全转录组表达的工具,它让研究人员能够检测疾病状态、治疗反应、各种环境条件下以及一系列其他研究设计中以前无法检测的变化。

RNA-Seq可使研究人员在单次分析中检测已知的和新的特征,使检测转录本亚型、基因融合、单核苷酸位点变异和其他特征不受先验知识的限制。

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用RNA-Seq推动进展

RNA测序可以对研究和创新产生深远的影响,改变我们对周围世界的理解。

RNA测序的优势

用新一代测序(NGS)开展RNA-Seq已逐渐成为研究人员研究转录组的首选方法。与基因表达芯片相比具有很多优势。

  • 更广泛的动态范围使基因表达的检测更加灵敏和准确
  • 为基因表达提供灵敏、准确的测量
  • 不受先验知识的限制——捕捉已知的和新的特征
  • 同时生成定性和定量数据
  • 适用于任何物种,即使在没有参考测序的情况下
  • 揭示了完整的转录组,而不仅仅是一些选定的转录组
通过RNA测序推进转录组学研究

了解RNA-Seq如何在各个领域推进转录组的研究,以及基因调控研究如何提供补充信息。

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RNA测序发表回顾

RNA-Seq 是迄今为止被引用最多的 NGS 方法,该合集采用 Illumina 技术的各种 RNA-Seq 方法的相关同行评审出版物。

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RNA测序发表回顾
刚刚接触NGS?

了解Illumina NGS技术的工作原理,以及它支持哪些类型的实验。

如何使用NGS分析RNA?

学习7种主要的RNA-Seq方法。找出它们的不同之处,帮助您确定适合您的研究方法。

主要RNA-Seq方法介绍

如何应用RNA-Seq?

癌症RNA-Seq研究基因表达和转录组的变化。

微生物RNA-Seq分析宿主-病原体相互作用或细菌转录组的特征。

研究药物反应RNA生物标记

经常一并购买

特色RNA测序文章

Precision Immunotherapies Using Tumor-Specific HLA Ligands
使用肿瘤特异性HLA配体的精准免疫疗法

RNA-Seq和HLA分型可提高靶点发现平台的性能和效率。

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The Time Is Now for Microbiome Studies
进行微生物组研究的好时机

转录组学和全基因组鸟枪法测序为研究人员和制药公司提供了重要数据,可助其改进药物的发现和开发。

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Circulating RNA
Cell-Free RNA as a Noninvasive Biomarker

This research highlights the broad potential of circulating cell-free RNA sequencing for biomarker discovery and noninvasive health monitoring.

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Library Prep for RNA Sequencing

RNA测序文库制备

RNA-Seq文库制备的进步正在彻底改变转录组的研究。我们的增强型RNA-Seq文库制备产品系列涵盖了多种类型的测序研究。这些解决方案可提供快速的周转时间、广泛的研究灵活性和测序可扩展性。

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Experimental Considerations for RNA-Seq

规划您的RNA-Seq实验

特色产品

Illumina Stranded mRNA Prep
Illumina Stranded mRNA Prep

一个简单、可扩展、经济、快速的解决方案,只需25 ng标准(未降解)RNA,即可在一天之内对编码转录组实现快速分析。

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NextSeq 1000 & 2000系统

这款经济且用户友好的中通量台式测序仪提供了极大的灵活性,可支持新兴应用。

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DRAGEN RNA Pipeline
DRAGEN RNA Pipeline

进行比对、定量和融合检测。

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RNA-Seq Differential Expression
RNA-Seq Differential Expression

实现差异基因表达分析。

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Yes, RNA-Seq is a well-established and accepted method for quantifying gene expression. To see how RNA-Seq compares against microarrays and quantitative PCR (qPCR), view the pages below:

Transcriptomics broadly refers to the study of RNA related to its expression levels, function, structure, and regulation. RNA-Seq is more specific and refers to the technique to study both the sequence and quantity of RNA.

RNA sequencing depth is the ratio of the total number of bases obtained by sequencing to the size of the genome or the average number of times each base is measured in the genome.

Bulk RNA-Seq is a method that analyzes pooled RNA from cells or tissues.

RNA sequencing strandedness allows researchers to determine which DNA strand (sense or antisense) a transcript came from. Compared to regular RNA sequencing methods, stranded RNA sequencing can find novel transcripts, distinguish transcripts from overlapping genes, find antisense sequences, and annotate genes.

Visit the Illumina Stranded mRNA Prep page for more information.

In mRNA library preparation methods, mRNA is selected via oligodT beads from total RNA, so libraries are prepared only from polyadenylated transcripts from samples. In total RNA workflows, rRNA and select other abundant transcripts are depleted from samples, and the remaining RNAs are prepared into sequencing libraries, including polyadenylated and non-polyadenylated RNAs. In enrichment workflows, libraries are prepared from all RNA samples. These libraries are subsequently enriched using an oligo probe panel. Panels can target full coding exomes, transcripts associated with specific diseases, RNA from pathogens, or custom targets. For more information on these workflows, visit the following pages:

Extracted RNA must be purified and free of contaminants. Illumina recommends following the guidelines provided in your particular extraction kit and selecting an appropriate protocol for your sample type.

The following provides input ranges of RNA for your selected method:

RNA-seq is compatible with all Illumina sequencers. Depending on the library preparation kit, the application, and the data needs, higher or lower throughput sequencers will be more appropriate. Contact your sales representative for more information.

Yes, RNA-seq library preparation methods can be automated. Visit our Library Prep Automation page for more information.

Yes, Illumina offers quality control solutions depending on your application. See our library quantification and QC reference guide for detailed information.

其他资源

RNA测序工作流程指南
RNA测序工作流程指南

了解Illumina新一代RNA测序应用解决方案。

常见问题:RNA-Seq数据分析
常见问题:RNA-Seq数据分析

解答最常咨询的问题,揭开数据分析的神秘面纱。

COVID-19宿主风险与宿主反应

宿主遗传差异和个体对 SARS-CoV-2 病毒的反应在疾病易感性和严重程度中发挥作用。

 

解锁多细胞组织的新空间视图

Visium 空间基因表达使您能够可视化覆盖基因活动的组织形态,揭示细胞之间的空间关系以及它们如何促进组织发育、功能和疾病状态。

 

References
  1. Wang Z, Gerstein M, Snyder M. RNA-Seq: a revolutionary tool for transcriptomics. Nat Rev Genet. 2009;10:57–63.
  2. Wilhelm BT, Landry JR. RNA-Seq—quantitative measurement of expression through massively parallel RNA sequencing. Methods. 2009;48:249–57.