Introduction to Targeted Resequencing
With targeted resequencing, a subset of genes or regions of the genome are isolated and sequenced. Targeted approaches using next-generation sequencing (NGS) allow researchers to focus time, expenses, and data analysis on specific areas of interest. Such targeted analysis can include the exome (the protein-coding portion of the genome), specific genes of interest (custom content), targets within genes, or mitochondrial DNA.
Benefits of Targeted Resequencing
Targeted resequencing is simpler and more accessible than ever before. Find out how to access the focused power of targeted NGS.
重点内容带来了经济实惠的测序
- 关注感兴趣的区域,生成更小、更容易管理的数据集
- 降低测序成本和数据分析的负担
- 与范围更广的方法相比加快了周转时间
- 以高覆盖度水平实现了深度测序，适合稀有变异的鉴定
与范围更广的方法相比，如全基因组测序，靶向测序是一种更加经济高效的方法，可研究感兴趣的区域。靶向重测序让研究人员能够将重点放在那些最有可能参与所研究的表型的区域，从而节省资源，并生成更小、更容易管理的数据集。靶向方法还能带来更高的覆盖度水平，从而鉴定那些稀有变异或对全基因组或桑格测序来说太过昂贵的变异。
Using qPCR or Sanger sequencing today?
Find out how Illumina targeted sequencing can help you discover more.
How can I use NGS to analyze specific genes or DNA regions?
Explore common targeted NGS methods, from exome to amplicon sequencing and more.
How can I apply targeted NGS?
Discover, validate, or screen genetic variants with targeted sequencing for various research areas, from cancer to microbiology and more.
Explore uses of targeted NGSTargeted Resequencing Applications
Cancer Gene Sequencing
Targeted sequencing studies focus on a select set of genes or gene regions that have known associations with cancer. The deep coverage offered by targeted resequencing allows for higher sensitivity to call variants in rare tumor subclones accurately. Learn more about targeted cancer sequencing.
Inherited Condition Studies
Sequencing genes with known involvement in certain conditions, such as autism or cardiovascular diseases, can detect the variants associated with inherited disorders. NGS could help reduce research costs and shorten the time to answer compared to traditional, iterative approaches. Learn more about genetic disease research.
Complex Disease Research
Mendelian disorders can result from rare genetic variants. Exome sequencing allows targeted exploration of protein-coding regions of the genome to identify the rare variants that contribute to complex genetic diseases. Learn more about complex disease genomics.
Drug Development
In the future, targeted sequencing might be used to validate drug targets and confirm variants of interest for cancer treatment and other therapeutics. Learn more about NGS in drug development.
Mitochondrial Disease Research
Targeted NGS enables researchers to identify and analyze variants associated with mitochondrial disorders. Learn more about mitochondrial sequencing.
Bacterial Identification
Sequencing the bacterial 16S ribosomal RNA gene is a common amplicon sequencing method used to identify and compare bacteria present within a given sample. It is useful for studying complex microbiomes or environmental samples. Learn more about 16S rRNA sequencing.
Environmental DNA Sequencing
Environmental DNA (eDNA) sequencing is a rapidly emerging method for studying biodiversity and monitoring ecosystem changes. Learn more about eDNA sequencing.
Featured Targeted Sequencing Workflow: Custom Amplicon Sequencing
This key method uses ultra-deep sequencing of PCR amplicons to target specific genomic regions.
Content Selection
DesignStudio Software Tool
Online tool makes it easy to optimize for target region coverage.
AmpliSeq for Illumina Custom DNA Panel
Create custom targeted sequencing panels optimized for content of interest.
Library Prep & Accessories
AmpliSeq for Illumina Library PLUS, Indexes & Accessories
Rapidly prepare amplicon libraries for Illumina sequencers.
Sequencing
iSeq 100 System
Affordable, fast, and accessible sequencing power for targeted or small genome sequencing in any lab.
MiniSeq System
Supports a broad range of targeted DNA and RNA applications.
Data Analysis
Local Run Manager (DNA Amplicon Analysis Module)
An on-premises software solution for creating sequencing runs, monitoring run status, and analyzing data.
BaseSpace Sequence Hub (DNA Amplicon App)
The Illumina genomics cloud computing environment for NGS data analysis and management.
Featured Publications
Somatic mutations in cerebral cortical malformations.
N Engl J Med 371 733-43 2014
Massively parallel multiplex DNA sequencing for specimen identification using an Illumina MiSeq platform.
Sci Rep 5 9687 2015
Identification of minority resistance mutations in the HIV-1 integrase coding region using next generation sequencing.
J Clin Virol 73 95-100 2015
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更多资源
在iSeq 100系统上使用的AmpliSeq for Illumina
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