AmpliSeq for Illumina Comprehensive Cancer Panel
靶向panel审查了409个已知与癌症相关的基因外显子区域。
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AmpliSeq for Illumina Comprehensive Cancer Panel提供了一种靶向重测序解决方案，用于分析涉及409个基因的体细胞突变，这些基因与癌症具有已知关联。主要特点包括：
Comprehensive Cancer Panel是集成工作流程的一部分，该工作流程包括基于AmpliSeq for Illumina聚合酶链式反应（PCR）的文库制备、采用Illumina边合成边测序（SBS）的新一代测序（NGS）技术以及自动化分析过程。
这种panel可检测肺癌、结肠癌、乳腺癌、卵巢癌、黑色素瘤和前列腺癌等多种癌症类型的变异。即用型panel助您节省鉴定目标基因、设计引物和优化panel所需的时间和精力。
| 仪器 | 推荐的样本数 | 读长 |
|---|---|---|
| NextSeq 550 System | Samples per run: mid output: 4, high output: 12 (assumes minimum coverage of 500×) | 2 × 150 bp |
| AmpliSeq for Illumina Comprehensive Cancer Panel | AmpliSeq for Illumina Cancer Hotspot Panel v2 | |
|---|---|---|
| Assay Time | 6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | |
| Content Specifications | Full exon coverage of 409 genes associated with cancer. | |
| Description | Somatic analysis research across pan-cancer genes. | |
| Hands-On Time | < 1.5 hours | |
| Input Quantity | 1–100 ng (10 ng recommended per pool) | |
| Multiplexing | 96 dual index combinations | |
| Species Category | Human | |
| 方法 | 扩增子测序, 靶向DNA测序 | 扩增子测序, 靶向DNA测序 |
| 物种类别 | 人类 | 人类 |
| 癌症类型 | 泛癌种, 实体瘤 | 泛癌种, 实体瘤 |
Automated Solutions for AmpliSeq for Illumina Sequencing Panels
Application Note | HTML
AmpliSeq for Illumina Comprehensive Cancer Panel Gene List
product_file | EXCEL < 1 MB
AmpliSeq for Illumina Comprehensive Cancer Panel Data Sheet
Data Sheet | HTML
NextSeq 550 amplicon sequencing solution
Application Note | PDF < 1 MB
Sample identification with the AmpliSeq for Illumina Sample ID Panel
Application Note | PDF < 1 MB
AmpliSeq for Illumina Comprehensive Cancer Panel Consumables & Equipment Documentation
AmpliSeq for Illumina Comprehensive Cancer Panel支持 - 文档与宣传资料 Documentation
AmpliSeq for Illumina Comprehensive Cancer Panel Reference Guide Documentation
Illumina Adapter Sequences Documentation
Index Adapters Pooling Guide Documentation
AmpliSeq for Illumina Direct FFPE DNA Kit Reference Guide Documentation
AmpliSeq for Illumina Comprehensive Cancer Panel Checklist Documentation
自定义协议选择器
Generates customized, end-to-end instructions
全部 AmpliSeq for Illumina Comprehensive Cancer Panel支持 Support
