Nextera XT DNA Library Preparation Kit

Prepare sequencing libraries for small genomes, PCR amplicons, plasmids, or cDNA in as little as 90 minutes, with a low DNA input requirement.Read More...
选择产品
What products do I need?
Library Prep

Nextera XT DNA Library Preparation Kit (24 samples)

FC-131-1024

Nextera XT DNA Library Preparation Kit (96 samples)

FC-131-1096


Index Adapters

Nextera XT Index Kit v2 Set A (96 indexes, 384 samples)

FC-131-2001

Nextera XT Index Kit v2 Set B (96 indexes, 384 samples)

FC-131-2002

Nextera XT Index Kit v2 Set C (96 indexes, 384 samples)

FC-131-2003

Nextera XT Index Kit v2 Set D (96 indexes, 384 samples)

FC-131-2004

Nextera XT Index Kit (24 indexes, 96 samples)

FC-131-1001

IDT® for Illumina Nextera DNA UD Indexes Set A (96 Indexes, 96 Samples)

20027213

配件产品
What accessories do I need?

Illumina Free Adapter Blocking Reagent (12 reactions)

20024144

Illumina Free Adapter Blocking Reagent (48 reactions)

20024145

Product Highlights

Fast Library Prep, Optimized for Small Genomes, PCR Amplicons, and Plasmids
  • Rapid library preparation – complete library prep in as little as 90 minutes with only 15 minutes of hands-on time
  • Fast time to results – go from DNA to data in 8 hours with our benchtop sequencers.
  • Optimized for small genomes, PCR amplicons, and plasmids – one library prep kit for many applications
  • Innovative sample normalization – eliminates the need for library quantification before sample pooling and sequencing

With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single tube enzymatic reaction. Nextera XT supports ultra-low DNA input of only 1 ng. It enables a wide range of input samples, including small genomes, PCR amplicons greater than 300 bp, plasmids, microbial genomes, concatenated amplicons, and double-stranded cDNA.

Multiplexing of up to 384 samples per Nextera XT library is also available for those projects requiring greater throughput. Plus, the kit includes an innovative bead-based sample normalization that eliminates the need for library quantification prior to pooling and sequencing. Libraries prepared with Nextera XT kits are compatible with all Illumina sequencers.

The IDT® for Illumina®-Nextera™ DNA UD Indexes Set A is the latest set of indexes, offering up to 96 unique dual indexes to enable accurate assignment of reads and efficient use of the flow cell.

The IDT for Illumina - Nextera DNA UD Indexes use 10 base pair (bp) index codes. This index code change requires adjustments to the sequencing run setup. See the Nextera DNA UD indexes support page for additional information.

Find automation vendors with robotic systems compatible with this kit

When to Use Standard vs. Bead-Based Normalization

For high-throughput research, bead-based normalization can save time and resources by providing an accelerated path from DNA to data. This technical note provides guidelines and data comparing the two normalization methods to help you decide which option to use.

Read Tech Note
When to Use Standard vs. Bead-Based Normalization

Frequently Purchased Together

规格

计划推荐

仪器 推荐的样本数 读长
MiSeq System 每次运行多达384个样本 长达 2 x 300 bp
NextSeq 550 System 每次运行多达384个样本 长达 2 x 150 bp

产品比较

Nextera XT DNA Library Preparation Kit Nextera DNA Flex Library Prep Kit
变异种类 单核苷酸多态性(SNP), 结构变异 基因融合, 单核苷酸多态性(SNP), 体细胞变异, 拷贝数变异(CNV), 杂合性缺失(LOH), 插入缺失, 染色体异常, 生殖系变异, 结构变异
技术 测序 测序
方法 从头测序 , 扩增子测序 , 16S rRNA测序, 全基因组测序 , 鸟枪法测序 鸟枪法测序 , 全基因组测序 , 扩增子测序 , 从头测序
核酸类型 DNA DNA
物种类别 所有物种, 植物, 小鼠, 大鼠, 哺乳动物, 病毒, 人类, 酵母, 细菌 人类, 所有物种, 病毒, 酵母, 植物, 大鼠, 细菌, 哺乳动物, 小鼠
特殊的样本类型 低起始量样本, 单细胞 唾液, 血液
系统兼容性 MiSeq , MiSeq, MiniSeq , NextSeq 550 , NextSeq 500 , 研究模式下的MiSeq FGx HiSeq X Five , MiniSeq , HiSeq 2500 , NextSeq 550 , MiSeq , MiSeq, HiSeq X Ten , 研究模式下的MiSeqDx , HiSeq 4000 , HiSeq 3000
自动化功能 自动液体处理设备 自动液体处理设备

Method-Specific Workflow Example

 

Customer Stories

绘制神经元多样性:小鼠初级视觉皮质中神经员标识的分子分析

通过 MiSeq和HiSeq系统执行的单细胞mRNA-Seq,艾伦研究所(Allen Institute)研究人员可分类V1神经元细胞。

了解更多
新一代测序帮助研究人员抗击埃博拉病毒

通过采用HiSeq and MiSeq系统进行基因组学研究,研究人员可跟踪埃博拉爆发并了解病毒快速突变的影响。

了解更多

Supporting Data and Figures

 

Related Products

Nextera DNA Flex Library Prep Kit

A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species.


Nextera Mate Pair Library Prep Kit

This library preparation kit offers a gel-free method for preparing up to 12 kb mate pair libraries with minimal DNA input requirements.


TruSeq DNA Nano

Generate whole-genome sequencing libraries and efficiently interrogate samples with limited available DNA.