De Novo测序介绍
De novo测序是指首次测序一种新颖的基因组，并需要测序reads的专门组装。读长、深度以及灵活的双端插入片段大小的独特组合让Illumina测序仪成为de novo测序的理想之选。无以伦比的原始read准确性让您自信、高效地产生高质量的长重叠群组装。.
与Sanger测序等传统方法相比，新一代测序（NGS）能够更快速、更准确地鉴定任何物种。Illumina提供了mate pair测序和long-read技术，作为短序列的补充，可准确、完整地鉴定任何物种。
De Novo Sequencing in 3 Steps
Assemble novel bacterial genomes with this simple workflow solution.
De Novo测序的优势
- 即使是复杂或多倍体基因组，也能生成准确的参考序列
- 为绘制新型生物的基因组图谱或完善已知生物的基因组提供有用的信息
- 阐明高度相似或重复的区域，便于准确的de novo组装
- 鉴定结构变异和复杂重排，如缺失、倒位或易位
准确的De Novo基因组组装
在首次测序基因组时，使用混合方法可实现更高质量的组装。将双端的短片段序列与mate pair的长片段序列相结合，是最大限度提高覆盖度的理想方式。以更高深度测序的短序列可填补长序列未覆盖的空白。
这种组合可实现最广泛的结构变异的检测，对复杂重排的准确鉴定也至关重要。合成的长序列通过提供由短序列“缝合”在一起而成的长contig，以保持准确性，从而协助组装。
Featured De Novo Sequencing Articles
Illumina Sequencers Help Characterize and Control Coronavirus
See how Illumina NGS was used for de novo sequencing and characterization of the novel coronavirus genome, in combination with other technologies, as published in the New England Journal of Medicine.
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Identifying Sweet Potato Viruses
Scientists at the Agricultural Research Council in South Africa use sequencing to identify known and novel sweet potato viruses, with the goal of enhancing food security.
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Advancing Species Research
Researchers at the Okinawa Institute of Science and Technology use de novo sequencing and other NGS methods to identify, characterize, and catalog the diversity of species on land and in the sea.
Read ArticleInvestigating the Genetics of COVID-19 Susceptibility
Illumina is providing sequencing for a UK-wide study led by Genomics England, designed to compare the genomes of severely and mildly ill COVID-19 patients. This research may help uncover genetic factors associated with susceptibility.
Read Article
De Novo细菌测序的建议流程
Assembly Report
Prokka Input Form
全面的De Novo 基因组测序流程
Illumina边合成边测序（SBS）技术是最广泛采用的NGS技术，生成了全球约90%的测序数据。*
除了业界领先的数据质量，Illumina也提供了从文库制备到数据分析的完整流程，可简化de novo测序。
单击下面的可查看每个工作流程步骤的产品。
Nextera Mate Pair Library Prep Kit
不使用凝胶和使用凝胶的方法，从少量DNA中制备测序用的mate pair文库。
10x Genomics Chromium Genome Library Prep KitWhole genome prep that provides variant calling and phasing for sequencing on Illumina platforms from low DNA input.
Dovetail ServiceDe novo assembly for a large range of genomes using the proprietary Dovetail Chicago TM method on Illumina platforms from multiple DNA inputs.
Long-Read Sequencing Technology
Highly accurate assembly of DNA fragments into long reads for whole-genome sequencing or genome phasing.
NRGene ServiceDe novo assembly of complex genomes for Ag researchers to deliver long, phased sequences and accurate assembly results on Illumina platforms.
iSeq 100系统
Fast, user-friendly, affordable sequencing that provides uniform coverage and genome assembly for microbial species.
MiSeq系统快速简约，适用于靶向和小型基因组测序。
NovaSeq 6000系统Scalable throughput and flexibility for virtually any genome, sequencing method, and scale of project.
Nextera Mate Pair
BaseSpace SPAdes Genome Assembler AppDe novo assembler suitable for single-cell and isolate genomes.
BaseSpace Velvet De Novo Assembly AppDe novo assembly of bacterial genomes using the Velvet assembler, with a focus on Nextera Mate Pair data.
Visualization
Integrative Genomics ViewerDisplays alignments and variants from multiple samples for performing complex variant analysis.
BaseSpace Sequence HubThe Illumina genomics computing environment for NGS data analysis and management.
NovaSeq 6000 Flow Cell Options
Reagent kits for the NovaSeq 6000 System are available in multiple flow cell configurations to support a broad range of applications. SP and S1 are ideal for small batch sizes or in situations where rapid turnaround time is required. S2 is a quick, powerful, and cost-effective option for high-throughput applications. S4 offers ultra-high throughput sequencing across a wide range of sequencing methods.
View Kits
相关解决方案
微生物测序
微生物全基因组测序是一种重要的工具，可以用来绘制新物种的基因组，完成已知物种的基因组，或比较多个样品的基因组。深入了解微生物全基因组测序。
肿瘤-正常测序
比较肿瘤及配对的正常样品的全基因组测序数据，为研究人员提供癌症机制的宝贵见解。深入了解肿瘤-正常测序。
宏基因组测序
鸟枪法宏基因组测序让研究人员可采样特定的复杂样品中存在的所有物种。通过这种方法，微生物学家可以评估细菌多样性，并研究那些无法培养的微生物，否则它们将难以或无法分析。深入了解鸟枪法宏基因组测序。
植物和动物测序
De novo测序是了解动植物功能及其与环境相互作用的遗传基础的第一步。一些研究人员利用组装好的基因组来分配图谱位置并堆叠不同品种的信息，用于后续的SNP发现。深入了解植物和动物测序。深入了解植物和动物测序。
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Additional Resources
Assembling the Kiwi Genome
Dr. Diana Le Duc uses de novo sequencing to understand the evolution of the kiwi bird.
De Novo Bacterial Sequencing
The MiSeq System is an ideal platform for microbial genome sequencing and de novo assembly.
*Data calculations on file. Illumina, Inc., 2015