货号为RS-122-2401、RS-122-2402和RS-122-2403的产品配置有更改。在新的配置中,文库制备试剂和标签接头均可单独购买。可单独购买的组件列表可在“选择产品”部分中找到。
RNA-Seq技术可针对编码和多种形式的非编码RNA实现均匀覆盖、精确测量链方向,发现的替代转录本、反义表达和等位基因特异性表达等特征可信度更高。TruSeq Stranded Total RNA with Ribo-Zero Plant集TruSeq RNA文库制备与Ribo-Zero核糖体RNA去除方法的优点于一体,为全转录组分析提供了强大、可扩展的解决方案。
TruSeq Stranded Total RNA with Ribo-Zero Plant能够特异且快速地去除叶片、种子和根部组织中的细胞质、线粒体和叶绿体核糖体RNA。这些产品已经过验证,可用于包括拟南芥、水稻和玉米在内的多种植物,并能够在大量的其他物种中有效地去除rRNA。
仪器 | 推荐的样本数 | 读长 |
---|---|---|
NextSeq 550 System | RNA Profiling: 6-20 samples per run (based on 20 million reads per sample) | 2 x 75 bp |
NextSeq 550 System | Transcriptome Analysis: 2-8 samples per run (based on 50 million reads per sample) | 2 x 75 bp |
HiSeq 2500 System | RNA Profiling: 30-200 samples per run (dual flow cell; based on 20 million reads per sample) | 2 x 75 bp |
HiSeq 2500 System | Transcriptome Analysis: 12-80 samples per run (dual flow cell; based on 50 million reads per sample) | 2 x 75 bp |
NovaSeq 6000 System | RNA Profiling (samples per run, dual flow cell): S1: 160, S2: 320, S4: 768. Limited by index combinations (dual). Based on 20 million reads. | ≤ 2 × 100 bp |
NovaSeq 6000 System | Transcriptome Analysis (samples per run, dual flow cell): S1: 64, S2: 128, S4: 400. Limited by index combinations (dual). Based on 50 million reads. | ≤ 2 × 100 bp |
Researchers at the ARC of South Africa use the MiSeq System to identify and map sweet potato viruses, with the goal of enhancing food security in developing countries.
Learn MoreData Sheet | PDF | 5 versions
Robust low-input RNA sequencing with TruSeq Stranded Total RNA Library Prep Gold
Application Note | HTML
TruSeq Stranded Total RNA Reference Guide Documentation
TruSeq Stranded Total RNA Consumables & Equipment Documentation
TruSeq Stranded Total RNA Documentation
TruSeq Stranded Total RNA Checklist Documentation
TruSeq Stranded Total RNA With Illumina Ribo-Zero Plus rRNA Depletion Reference Guide Documentation
自定义协议选择器
Generates customized, end-to-end instructions
有限标签使用许可:TruSeq Stranded Total RNA with Ribo-Zero Plant工作流程的产品及其使用受Max Planck Gesellschaft所拥有且独家授权给New England Biolabs, Inc.并再授权给Illumina, Inc.的一个或多个已公告和/或待批准的美国和外国专利申请的约束。 向Illumina, Inc.、其关联机构或其授权经销商和分销商购买此产品,即向买方转让使用买方所购数量产品和产品组件的权利(不论买方是学术性还是盈利性实体),该权利不可转让。 购买本产品不会获得上述专利或专利申请权利请求项下的产品生产许可。 买方不得向第三方出售或以其他方式转让此产品及其组件,亦不得以其他方式将此产品用于下商业用途:(1)在生产中使用此产品或其组件;或(2)出于治疗或预防疾病的目的,在人或动物身上使用此产品或其组件。