The products previously known as TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat (Cat. No. RS-122-2201, RS-122-2202, and RS-122-2203) and TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold (Cat. No. RS-122-2301, RS-122-2302, and RS-122-2303) are now called TruSeq Stranded Total RNA Human/Mouse/Rat and TruSeq Stranded Total RNA Gold, respectively. The product configuration has changed. In the new configuration, the library preparation reagents and index adapters can be purchased separately. The list of components available for individual purchase can be found in the “Select Product(s)” section.

TruSeq Stranded Total RNA

A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and formalin-fixed, paraffin-embedded (FFPE) tissue.Read More...
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Library Prep

TruSeq® Stranded Total RNA Library Prep Human/Mouse/Rat (48 Samples)

20020596

TruSeq® Stranded Total RNA Library Prep Human/Mouse/Rat (96 Samples)

20020597

TruSeq Stranded Total RNA Library Prep Gold (48 Samples)

20020598

TruSeq® Stranded Total RNA Library Prep Gold (96 Samples)

20020599


Index Adapters

TruSeq RNA CD Index Plate (96 Indexes, 96 Samples)

20019792

TruSeq RNA Single Indexes Set A (12 Indexes, 48 Samples)

20020492

TruSeq RNA Single Indexes Set B (12 Indexes, 48 Samples)

20020493

IDT for Illumina – TruSeq RNA UD Indexes (24 Indexes, 96 Samples)

20020591

IDT for Illumina – TruSeq RNA UD Indexes (96 Indexes, 96 Samples)

20022371

配件产品

Illumina Free Adapter Blocking Reagent (12 reactions)

20024144

Illumina Free Adapter Blocking Reagent (48 reactions)

20024145

Product Highlights

TruSeq Stranded Total RNA provides a clear view of the transcriptome with a streamlined, cost-efficient, and scalable solution for total RNA analysis with sequencing.

Whole-Transcriptome Analysis with Precise Measurement of Strand Orientation

Compatible with a wide range of samples, including low-quality DNA/RNA and formalin-fixed, paraffin-embedded (FFPE) samples, TruSeq Stranded Total RNA couples all of the benefits of TruSeq RNA technology with Ribo-Zero ribosomal RNA reduction chemistry. The product enables analysis of coding and multiple forms of noncoding RNA with precise measurement of strand orientation, uniform coverage, and high-confidence discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.

Species Compatibility

TruSeq Stranded Total RNA is designed for human, mouse and rat sequences, but may be applicable to a variety of eukaryotic species.*

*For information about a particular species of interest, contact Illumina Technical Support.

Flexible Solutions for a Range of Study Designs

TruSeq Stranded Total RNA is available in multiple versions to fit specific study design needs:

  • TruSeq Stranded Total RNA Human/Mouse/Rat removes cytoplasmic rRNA
  • TruSeq Stranded Total RNA Gold removes both cytoplasmic and mitochondrial rRNA
  • The total RNA kits are also compatible with the new unique dual (UD) index kits, IDT for Illumina - TruSeq RNA UD Indexes, which allows multiplexing of up to 96 samples

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Frequently Purchased Together

规格

计划推荐

仪器 推荐的样本数 读长
NextSeq 550 System RNA分析:每次运行6–20个样本(基于每个样本2000万条read)
转录组分析:每次运行2–8个样本(基于每个样本5000万条read)
2 x 75 bp
HiSeq 2500 System RNA分析:每次运行30–200个样本(双流动槽;基于每个样本2000万条read)
转录组分析:每次运行12–80个样本(双流动槽;基于每个样本5000万条read)
2 x 75 bp
NovaSeq 6000 System RNA分析(每次运行的样本,双流动槽):S1:160,S2:320,S4:768。受标签组合的限制(双重)。基于2000万条read。
转录组分析(每次运行的样本,双流动槽):S1:64,S2:128,S4:400。受标签组合的限制(双重)。基于5000万条read。
≤ 2 × 100 bp

产品比较

TruSeq Stranded Total RNA TruSeq Stranded Total RNA with Ribo-Zero Globin AmpliSeq for Illumina Transcriptome Human Gene Expression Panel
内容说明书 捕获编码RNA以及多种形式的非编码RNA。 捕获编码RNA以及多种形式的非编码RNA。 > 95%的人类RefSeq基因;20,802个基因;捕获人类转录组
变异种类 转录变异, 基因融合, 单核苷酸多态性(SNP), 新型转录本 新型转录本, 转录变异, 单核苷酸多态性(SNP), 基因融合 转录变异
技术 测序 测序 测序
方法 全转录组测序 全转录组测序 外显子组测序 , mRNA测序 , 靶向RNA测序
核酸类型 RNA RNA RNA
物种类别 大鼠, 人类, 小鼠 大鼠, 人类, 小鼠 人类
特殊的样本类型 FFPE组织 血液 血液, FFPE组织
系统兼容性 HiSeq 1000, HiSeq 2000, NextSeq 550 , HiSeq 1500, HiSeq 3000 , NextSeq 500 , HiSeq 4000 , HiSeq 2500 NextSeq 550 , HiSeq 4000 , NextSeq 500 , HiSeq 2500 , HiSeq 1500, HiSeq 3000 , HiSeq 2000, HiSeq 1000 NextSeq 550
自动化功能 自动液体处理设备 自动液体处理设备
起始量 0.1 – 1 ug 高质量总RNA。低质量样本可能需要进一步优化。 0.1 – 1 ug 血液来源的高质量纯化总RNA 1-100 ng RNA(推荐10 ng)

Method-Specific Workflow Example

 

Customer Stories

BRC-Seq客户从简单的BaseSpace集成式工作流程受益

通过BaseSpace Sequence Hub SRA Submission App,可在从文库制备到数据分析的整个过程跟踪样本,帮助BRC-Seq为其客户提供高质量测序数据和其他价值。

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Supporting Data and Figures

 
寻找合适的试剂盒

根据您的起始材料和感兴趣的方法,确定能够满足您需求的最佳文库制备试剂盒或芯片。

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TruSeq链式mRNA文库制备试剂盒

此试剂盒可从mRNA制备测序文库,以提供包含精确链信息的编码转录组视图。


Limited Use Label License: This product and its use are the subject of one or more issued and/or pending U.S. and foreign patent applications owned by Max Planck Gesellschaft, exclusively licensed to New England Biolabs, Inc. and sublicensed to Illumina, Inc. The purchase of this product from Illumina, Inc., its affiliates, or its authorized resellers and distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product by the buyer (whether the buyer is an academic or for profit entity). The purchase of this product does not convey a license under any claims in the foregoing patents or patent applications direct to producing the product. The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use the product for the following COMMERICAL PURPOSES: (I) use of the product or its components in manufacturing; or (2) use of the product or its components for therapeutic or prophylactic purposes in human or animals.