The products previously known as TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat (Cat. No. RS-122-2201, RS-122-2202, and RS-122-2203) and TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold (Cat. No. RS-122-2301, RS-122-2302, and RS-122-2303) are now called TruSeq Stranded Total RNA Human/Mouse/Rat and TruSeq Stranded Total RNA Gold, respectively. The product configuration has changed. In the new configuration, the library preparation reagents and index adapters can be purchased separately. The list of components available for individual purchase can be found in the “Select Product(s)” section.

TruSeq Stranded Total RNA

A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and formalin-fixed, paraffin-embedded (FFPE) tissue.Read More...
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Library Prep

TruSeq® Stranded Total RNA Library Prep Human/Mouse/Rat (48 Samples)

20020596

TruSeq® Stranded Total RNA Library Prep Human/Mouse/Rat (96 Samples)

20020597

TruSeq Stranded Total RNA Library Prep Gold (48 Samples)

20020598

TruSeq® Stranded Total RNA Library Prep Gold (96 Samples)

20020599


Index Adapters

TruSeq RNA CD Index Plate (96 Indexes, 96 Samples)

20019792

TruSeq RNA Single Indexes Set A (12 Indexes, 48 Samples)

20020492

TruSeq RNA Single Indexes Set B (12 Indexes, 48 Samples)

20020493

IDT for Illumina – TruSeq RNA UD Indexes (24 Indexes, 96 Samples)

20020591

IDT for Illumina – TruSeq RNA UD Indexes (96 Indexes, 96 Samples)

20022371

配件产品

Illumina Free Adapter Blocking Reagent (12 reactions)

20024144

Illumina Free Adapter Blocking Reagent (48 reactions)

20024145

Product Highlights

TruSeq Stranded Total RNA provides a clear view of the transcriptome with a streamlined, cost-efficient, and scalable solution for total RNA analysis with sequencing.

Whole-Transcriptome Analysis with Precise Measurement of Strand Orientation

Compatible with a wide range of samples, including low-quality DNA/RNA and formalin-fixed, paraffin-embedded (FFPE) samples, TruSeq Stranded Total RNA couples all of the benefits of TruSeq RNA technology with Ribo-Zero ribosomal RNA reduction chemistry. The product enables analysis of coding and multiple forms of noncoding RNA with precise measurement of strand orientation, uniform coverage, and high-confidence discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.

Species Compatibility

TruSeq Stranded Total RNA is designed for human, mouse and rat sequences, but may be applicable to a variety of eukaryotic species.*

*For information about a particular species of interest, contact Illumina Technical Support.

Flexible Solutions for a Range of Study Designs

TruSeq Stranded Total RNA is available in multiple versions to fit specific study design needs:

  • TruSeq Stranded Total RNA Human/Mouse/Rat removes cytoplasmic rRNA
  • TruSeq Stranded Total RNA Gold removes both cytoplasmic and mitochondrial rRNA
  • The total RNA kits are also compatible with the new unique dual (UD) index kits, IDT for Illumina - TruSeq RNA UD Indexes, which allows multiplexing of up to 96 samples

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规格

计划推荐

仪器 推荐的样本数 读长
NextSeq 550 System RNA分析:每次运行6–20个样本(基于每个样本2000万条read)
转录组分析:每次运行2–8个样本(基于每个样本5000万条read)
2 x 75 bp
HiSeq 2500 System RNA分析:每次运行30–200个样本(双流动槽;基于每个样本2000万条read)
转录组分析:每次运行12–80个样本(双流动槽;基于每个样本5000万条read)
2 x 75 bp
NovaSeq 6000 System RNA分析(每次运行的样本,双流动槽):S1:160,S2:320,S4:768。受标签组合的限制(双重)。基于2000万条read。
转录组分析(每次运行的样本,双流动槽):S1:64,S2:128,S4:400。受标签组合的限制(双重)。基于5000万条read。
≤ 2 × 100 bp

产品比较

TruSeq Stranded Total RNA TruSeq Stranded Total RNA with Ribo-Zero Globin AmpliSeq for Illumina Transcriptome Human Gene Expression Panel
核酸类型 RNA RNA RNA
物种类别 大鼠, 人, 小鼠 人, 大鼠, 小鼠
特殊的样本类型 FFPE Tissue FFPE Tissue

Method-Specific Workflow Example

 

Customer Stories

BRC-Seq客户从简单的BaseSpace集成式工作流程受益

通过BaseSpace Sequence Hub SRA Submission App,可在从文库制备到数据分析的整个过程跟踪样本,帮助BRC-Seq为其客户提供高质量测序数据和其他价值。

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Supporting Data and Figures

 
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Related Products

TruSeq Stranded Total RNA with Ribo-Zero Globin

Prepare whole-transcriptome sequencing libraries from blood-derived RNA with an efficient workflow that removes ribosomal RNA and globin mRNA in a single step.


TruSeq Stranded Total RNA with Ribo-Zero Plant

利用该RNA-Seq文库制备工作流程可全面了解植物转录组。可去除植物叶片、种子和根部组织中的rRNA。


TruSeq Stranded mRNA

从mRNA制备测序文库,获得清晰的编码转录组视图以及链特异性信息。


Limited Use Label License: This product and its use are the subject of one or more issued and/or pending U.S. and foreign patent applications owned by Max Planck Gesellschaft, exclusively licensed to New England Biolabs, Inc. and sublicensed to Illumina, Inc. The purchase of this product from Illumina, Inc., its affiliates, or its authorized resellers and distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product by the buyer (whether the buyer is an academic or for profit entity). The purchase of this product does not convey a license under any claims in the foregoing patents or patent applications direct to producing the product. The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use the product for the following COMMERICAL PURPOSES: (I) use of the product or its components in manufacturing; or (2) use of the product or its components for therapeutic or prophylactic purposes in human or animals.