TruSeq RNA Exome
Provides a reproducible, economical solution for sequencing RNA from FFPE tissues and other low-quality samples. Accuracy from as little as 10 ng total RNA.Read More...
The product previously known as the TruSeq RNA Access Library Prep Kit (Cat. No. RS-301-2001 and RS-301-2002) is now called TruSeq RNA Exome. The product configuration has changed. In the new configuration, major components such as library preparation, index adapters, enrichment reagents, and exome panels can be purchased separately. If you were previously buying Cat. No. RS-301-2001 or Cat. No. RS-301-2002, purchase the following components: TruSeq RNA Library Prep for Enrichment (Cat. No. 20020189), TruSeq RNA Enrichment (Cat. No. 20020490), Exome Panel (Cat. No. 20020183), and one item from the Index Adapters section below.
TruSeq RNA Exome, previously known as the TruSeq RNA Access Library Prep Kit, converts total RNA into template molecules of known strand origin, followed by sequence-specific capture of coding RNA. This provides a low-cost solution for analyzing human RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissues and other low-quality samples.
TruSeq RNA Exome generates RNA sequencing (RNA-Seq) libraries from degraded samples that focus on the RNA coding regions. Isolating these high-value content regions to help maximize discovery power, while requiring only a fraction of the read depth of total RNA sequencing. The results are low input requirements, high sample throughput, and cost-effective transcriptome analysis. Reagent volumes supplied are sufficient to support 1- to 4-plex enrichment reactions.
Find automation vendors with robotic systems compatible with this product
| 仪器 | 推荐的样本数 | 读长 |
|---|---|---|
| NextSeq 550 System | 每次运行5至16个样本（基于每个样本2500万条read） | 2 x 75 bp |
| HiSeq 2500 System | 每次运行24–160个样本（双流动槽；基于每个样本2500万条read） | 2 x 75 bp |
| NovaSeq 6000 System | 每次运行的样本（双流动槽）：S1：96，S2：96，S4：192（目前受可用标签组合的限制） | 2 × 100 bp |
| TruSeq RNA Exome | TruSeq RNA Library Prep Kit v2 | TruSeq链式mRNA文库制备试剂盒 | SureCell WTA 3′ Library Prep Kit for the ddSEQ System | |
|---|---|---|---|---|
| 技术 | 测序 | 测序 | 测序 | 测序 |
| 方法 | mRNA测序 , 外显子组测序 | mRNA测序 | mRNA测序 | mRNA测序 |
| 核酸类型 | RNA | RNA | RNA | RNA |
| 物种类别 | 人类 | 哺乳动物, 大鼠, 人类, 牛, 小鼠 | 大鼠, 人类, 小鼠, 牛, 哺乳动物, 其他 | 人类, 小鼠 |
| 系统兼容性 | HiSeq 4000 , NextSeq 500 , HiSeq 3000 , HiSeq 2500 , NextSeq 550 | HiSeq 1000, HiSeq 4000 , NextSeq 550 , HiSeq 2000, HiSeq 1500, MiSeq , MiSeq, NextSeq 500 , Genome Analyzer IIx, HiSeq 3000 , HiSeq 2500 | HiSeq 3000 , Genome Analyzer IIx, NextSeq 500 , HiSeq 4000 , MiSeq , MiSeq, HiSeq 1500, HiSeq 2500 , HiSeq 2000, NextSeq 550 , HiSeq 1000 | NextSeq 550 , NextSeq 500 , HiSeq 4000 , HiSeq 1500, HiSeq 3000 , HiSeq 1000, HiSeq 2000, HiSeq 2500 |
TruSeq RNA Exome enables detection of expressed fusion transcripts without the need to design probes specific for the fusion junction. The well-characterized BCR-ABL fusion is detected efficiently in the Universal Human Reference RNA (UHRR) sample at 25 M reads.
Evaluating RNA Quality from FFPE Samples
Technical Note | PDF1 MB
Robust low-input RNA sequencing with TruSeq Stranded Total RNA Library Prep Gold
Application Note | HTML
RNA-Seq Offers Significant Advantages in Immuno-Oncology Research
White Paper | PDF2 MB
Data Sheet | PDF | 5 versions
TruSeq RNA Exome Reference Guide Documentation
TruSeq RNA Exome Consumables & Equipment Documentation
TruSeq RNA Exome Documentation
TruSeq RNA Exome Checklist Documentation
自定义协议选择器
Generates customized, end-to-end instructions
Targeted panel that measures expression levels of >20,000 human RefSeq genes.
Targeting 1385 cancer genes for gene expression, variant, and fusion detection studies in many RNA sample types including blood, bone marrow, and formalin-fixed, paraffin-embedded (FFPE) tissue.
