The product previously known as the TruSeq RNA Access Library Prep Kit (Cat. No. RS-301-2001 and RS-301-2002) is now called TruSeq RNA Exome. The product configuration has changed. In the new configuration, major components such as library preparation, index adapters, enrichment reagents, and exome panels can be purchased separately. If you were previously buying Cat. No. RS-301-2001 or Cat. No. RS-301-2002, purchase the following components: TruSeq RNA Library Prep for Enrichment (Cat. No. 20020189), TruSeq RNA Enrichment (Cat. No. 20020490), Exome Panel (Cat. No. 20020183), and one item from the Index Adapters section below.

TruSeq RNA Exome

Provides a reproducible, economical solution for sequencing RNA from FFPE tissues and other low-quality samples. Accuracy from as little as 10 ng total RNA.Read More...
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Library Prep

TruSeq® RNA Library Prep for Enrichment (48 Samples)

20020189


Enrichment

TruSeq® RNA Enrichment (12 enrichments)

20020490


Index Adapters

TruSeq RNA Single Indexes Set A (12 Indexes, 48 Samples)

20020492

TruSeq RNA Single Indexes Set B (12 Indexes, 48 Samples)

20020493


Panel

Illumina Exome Panel - Enrichment Oligos only (8 or 12 Enrichment Reactions)

20020183

配件产品

Illumina Free Adapter Blocking Reagent (12 reactions)

20024144

Illumina Free Adapter Blocking Reagent (48 reactions)

20024145

Product Highlights

TruSeq RNA Exome, previously known as the TruSeq RNA Access Library Prep Kit, converts total RNA into template molecules of known strand origin, followed by sequence-specific capture of coding RNA. This provides a low-cost solution for analyzing human RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissues and other low-quality samples.

  • Affordability and focus—Isolating human transcriptome coding regions maximizes discovery power at a fraction of the sequencing depth
  • High-quality data from difficult samples—Optimized for sequencing RNA from degraded samples, including FFPE tissues
  • Samples with limited starting material—Greatly reduced sample input requirements (as little as 10 ng total RNA from fresh or frozen samples or 20 ng total RNA from degraded samples) while maintaining high sensitivity

TruSeq RNA Exome generates RNA sequencing (RNA-Seq) libraries from degraded samples that focus on the RNA coding regions. Isolating these high-value content regions to help maximize discovery power, while requiring only a fraction of the read depth of total RNA sequencing. The results are low input requirements, high sample throughput, and cost-effective transcriptome analysis. Reagent volumes supplied are sufficient to support 1- to 4-plex enrichment reactions.

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规格

计划推荐

仪器 推荐的样本数 读长
NextSeq 550 System 每次运行5至16个样本(基于每个样本2500万条read) 2 x 75 bp
HiSeq 2500 System 每次运行24–160个样本(双流动槽;基于每个样本2500万条read) 2 x 75 bp
NovaSeq 6000 System 每次运行的样本(双流动槽):S1:96,S2:96,S4:192(目前受可用标签组合的限制) 2 × 100 bp

产品比较

TruSeq RNA Exome TruSeq RNA Library Prep Kit v2 TruSeq链式mRNA文库制备试剂盒 SureCell WTA 3′ Library Prep Kit for the ddSEQ System
技术 测序 测序 测序 测序
方法 mRNA测序 , 外显子组测序 mRNA测序 mRNA测序 mRNA测序
核酸类型 RNA RNA RNA RNA
物种类别 人类 哺乳动物, 大鼠, 人类, 牛, 小鼠 大鼠, 人类, 小鼠, 牛, 哺乳动物, 其他 人类, 小鼠
系统兼容性 HiSeq 4000 , NextSeq 500 , HiSeq 3000 , HiSeq 2500 , NextSeq 550 HiSeq 1000, HiSeq 4000 , NextSeq 550 , HiSeq 2000, HiSeq 1500, MiSeq , MiSeq, NextSeq 500 , Genome Analyzer IIx, HiSeq 3000 , HiSeq 2500 HiSeq 3000 , Genome Analyzer IIx, NextSeq 500 , HiSeq 4000 , MiSeq , MiSeq, HiSeq 1500, HiSeq 2500 , HiSeq 2000, NextSeq 550 , HiSeq 1000 NextSeq 550 , NextSeq 500 , HiSeq 4000 , HiSeq 1500, HiSeq 3000 , HiSeq 1000, HiSeq 2000, HiSeq 2500

Method-Specific Workflow Example

 

Customer Stories

寻找除单药法以外的精准肿瘤学研究方法

研究人员依赖于转录组来揭示多药物敏感性致瘤通路。

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Supporting Data and Figures

Efficient Gene Fusion Discovery
Supporting Data and Figures

TruSeq RNA Exome enables detection of expressed fusion transcripts without the need to design probes specific for the fusion junction. The well-characterized BCR-ABL fusion is detected efficiently in the Universal Human Reference RNA (UHRR) sample at 25 M reads.

 

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