TruSeq DNA Nano
Generate whole-genome sequencing libraries and efficiently interrogate samples with limited available DNA.Read More...
To provide greater flexibility in ordering single components for library preparation, we are moving to a modular configuration. Our existing TruSeq Nano DNA Library Prep Kits (Cat. No. FC-121-4001, FC-121-4002, and FC-121-4003) will transition to TruSeq DNA Nano (Cat. No. 20015964 for 24 samples and Cat. No. 20015965 for 96 samples) for library preparation. Index adapters will be sold separately as TruSeq DNA Single Indexes (Cat. No. 20015960 for Set A and Cat. No. 20015961 for Set B) and TruSeq DNA CD (combinatorial dual) Indexes (Cat. No. 20015949).
TruSeq DNA Nano enables efficient interrogation of samples with limited available DNA. Based on mechanical fragmentation–ligation chemistry, this low-input method delivers high coverage quality and reduced bias for virtually any sequencing application.
TruSeq DNA Nano has been streamlined by replacing gel-based size selection with bead-based selection, enabling researchers to prepare high-quality libraries in less than a day. It is optimized for a variety of read lengths, from 2 × 101 bp to 2 × 151 bp.
TruSeq DNA Nano offers excellent results with as little as 100 ng input, eliminating the typical requirement for micrograms of DNA. This enables researchers to study samples with limited available DNA (eg, tumor samples) and helps preserve samples for use in future or alternate studies. In addition to accelerating the workflow, bead-based size selection avoids typical sample loss associated with gel-based selection.
TruSeq DNA Nano reduces the number and average size of typical PCR-induced gaps in coverage. The enhanced workflow reduces library bias and improves coverage uniformity across the genome. These kits also provide excellent coverage of traditionally challenging genomic content, including GC-rich regions, promoters, and repetitive regions. This enables researchers to access more information from each sequencing run. Truseq DNA Nano products are validated for high-quality genomic coverage for virtually any next-generation sequencing application.
View a list of automation vendors with robotic systems that support the 96-sample workflow
| 仪器 | 推荐的样本数 | 读长 |
|---|---|---|
| NextSeq 550 System | 每次运行1个样本（基于30倍的人类基因组覆盖度） | 350bp 插入片段: ≤ 2 × 100 bp 550 bp 插入片段: ≤ 2 × 150 bp |
| HiSeq 2500 System | 每次运行2–10个样本（双流动槽；基于30倍的人类基因组覆盖度） | 快速运行, 350 bp 插入片段: ≤ 2 × 100 bp 快速运行, 550 bp 插入片段: ≤ 2 × 250 bp 高通量, 350 bp 插入片段: ≤ 2 × 100 bp 高通量, 550 bp 插入片段: ≤ 2 × 125 bp |
| TruSeq DNA Nano | TruSeq DNA PCR-Free | Nextera DNA Flex Library Prep Kit | |
|---|---|---|---|
| 变异种类 | 插入缺失, 拷贝数变异（CNV）, 杂合性缺失（LOH）, 染色体异常, 基因融合, 单核苷酸多态性（SNP）, 体细胞变异, 生殖系变异, 结构变异 | 染色体异常, 结构变异, 生殖系变异, 体细胞变异, 杂合性缺失（LOH）, 拷贝数变异（CNV）, 单核苷酸多态性（SNP）, 基因融合 | 基因融合, 单核苷酸多态性（SNP）, 体细胞变异, 拷贝数变异（CNV）, 杂合性缺失（LOH）, 插入缺失, 染色体异常, 生殖系变异, 结构变异 |
| 技术 | 测序 | 测序 | 测序 |
| 方法 | 鸟枪法测序 , 通过测序进行基因分型 , 全基因组测序 | 鸟枪法测序 , 通过测序进行基因分型 , 全基因组测序 | 鸟枪法测序 , 全基因组测序 , 扩增子测序 , 从头测序 |
| 核酸类型 | DNA | DNA | DNA |
| 物种类别 | 植物, 大鼠, 小鼠, 哺乳动物, 人类, 其他 | 小鼠, 大鼠, 哺乳动物, 人类, 植物, 其他 | 人类, 所有物种, 病毒, 酵母, 植物, 大鼠, 细菌, 哺乳动物, 小鼠 |
| 系统兼容性 | MiniSeq , HiSeq X Ten , HiSeq 4000 , HiSeq 3000 , HiSeq X Five , HiSeq 1500, MiSeq , MiSeq, NextSeq 550 , NextSeq 500 , HiSeq 2000, 研究模式下的MiSeqDx , HiSeq 1000, HiSeq 2500 | MiSeq , MiSeq, 研究模式下的MiSeqDx , MiniSeq , NextSeq 550 , HiSeq 2000, HiSeq X Ten , NextSeq 500 , HiSeq 2500 , HiSeq 1000, HiSeq 4000 , HiSeq 3000 , HiSeq X Five , HiSeq 1500 | HiSeq X Five , MiniSeq , HiSeq 2500 , NextSeq 550 , MiSeq , MiSeq, HiSeq X Ten , 研究模式下的MiSeqDx , HiSeq 4000 , HiSeq 3000 |
| 自动化功能 | 自动液体处理设备 | 自动液体处理设备 | 自动液体处理设备 |
| Specification | TruSeq DNA Nano | TruSeq DNA PCR-Free |
|---|---|---|
| Kit type | TruSeq DNA Nano LT & HT | TruSeq DNA PCR-Free LT & HT |
| Description | Based upon widely adopted TruSeq library prep, with lower input and improved data quality | Superior genomic coverage with radically reduced library bias and gaps |
| Workflow | LT kits: Manual workflow
HT kits: Automation-friendly; manual protocols also available |
LT kits: Manual workflow
HT kits: Automation-friendly; manual protocols also available |
| Input quantity | 100–200 ng
|
1–2 μg |
| Includes PCR | Yes | No |
| Assay time | 5.5 hours | ~5 hours |
| Hands-on time | 4 hours | ~4 hours |
| Target insert size | 350 bp or 550 bp | 350 bp or 550 bp |
| Gel-free | Yes | Yes |
| Number of samples supported | 24 (LT) or 96 (HT) samples | 24 (LT) or 96 (HT) samples |
| Size-selection beads | Included | Included |
| Applications | Whole-genome sequencing applications, including whole-genome resequencing, de novo assembly, and metagenomics studies | |
| Sample multiplexing | Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations | |
| Compatible Illumina sequencers | MiniSeq, MiSeq, NextSeq, HiSeq, NovaSeq, HiSeq X, HiScanSQ, and Genome Analyzer systems | |
Comparison of TruSeq Sample Preparation Kits
Technical Note | PDF< 1 MB
Mechanical DNA Fragmentation with the Q800R2 Sonicator
Technical Note | PDF4 MB
Data Sheet | PDF11 MB
TruSeq DNA Nano Consumables & Equipment Documentation
Illumina Experiment Manager User Guide Documentation
TruSeq DNA Nano Reference Guide Documentation
TruSeq Nano DNA Library Prep Reference Guide Documentation
TruSeq Nano DNA Library Prep User-Supplied Consumables and Equipment Documentation
TruSeq HT Dual Index Quick Reference Card Documentation
TruSeq DNA Nano Checklist Documentation
TruSeq Nano DNA Library Prep Checklist Documentation
自定义协议选择器
Generates customized, end-to-end instructions
Simple, all-inclusive whole-genome sequencing (WGS) library preparation that provides accurate and comprehensive coverage of complex genomes.
Prepare sequencing libraries for small genomes, PCR amplicons, plasmids, or cDNA in as little as 90 minutes, with a low DNA input requirement.
A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species.
