TruSeq DNA Nano
Generate whole-genome sequencing libraries and efficiently interrogate samples with limited available DNA.
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Illumina DNA Prep and Illumina DNA PCR-Free Prep are available. These library prep solutions offer improved performance while reducing turnaround time and hands-on time compared to TruSeq DNA Nano.
TruSeq DNA Nano enables efficient interrogation of samples with limited available DNA. Based on mechanical fragmentation–ligation chemistry, this low-input method delivers high coverage quality and reduced bias for virtually any sequencing application.
TruSeq DNA Nano has been streamlined by replacing gel-based size selection with bead-based selection, enabling researchers to prepare high-quality libraries in less than a day. It is optimized for a variety of read lengths, from 2 × 101 bp to 2 × 151 bp.
TruSeq DNA Nano offers excellent results with as little as 100 ng input, eliminating the typical requirement for micrograms of DNA. This enables researchers to study samples with limited available DNA (eg, tumor samples) and helps preserve samples for use in future or alternate studies. In addition to accelerating the workflow, bead-based size selection avoids typical sample loss associated with gel-based selection.
TruSeq DNA Nano reduces the number and average size of typical PCR-induced gaps in coverage. The enhanced workflow reduces library bias and improves coverage uniformity across the genome. These kits also provide excellent coverage of traditionally challenging genomic content, including GC-rich regions, promoters, and repetitive regions. This enables researchers to access more information from each sequencing run. Truseq DNA Nano products are validated for high-quality genomic coverage for virtually any next-generation sequencing application.
View a list of automation vendors with robotic systems that support the 96-sample workflow
| TruSeq DNA Nano | TruSeq DNA PCR-Free | Illumina DNA Prep | |
|---|---|---|---|
| Assay Time | ~6 hours total assay time | 5 hours total assay time | ~3-4 hours (from DNA extraction to normalized library) |
| Automation Capability | 液体处理机器人 | 液体处理机器人 | 液体处理机器人 |
| Description | A low-input research method that delivers high genome coverage quality and reduced bias. | A simple, all-inclusive PCR-free prep for whole-genome sequencing studies with the ability to sequence through challenging regions of the genome. | A fast, flexible workflow for a wide range of research applications and sample types, from human to microbial whole-genome sequencing and more. |
| Hands-On Time | ~4 hours | 4 hours | 1-1.5 hours |
| Input Quantity | 100 ng genomic DNA | 1 ug DNA | Small genomes (e.g. microbial): 1-500 ng DNA. Large genomes (e.g. human): 100-500 ng DNA. (For blood and saliva, see the reference guide). |
| Mechanism of Action | Mechanical DNA fragmentation and adapter ligation. Workflow uses reduced-bias PCR and is gel-free. | Mechanical DNA fragmentation and adapter ligation. Workflow is PCR-free and gel-free. | 连接磁珠的转座酶 |
| Multiplexing | Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations (available soon) | Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations (available soon) | Up to 384 unique dual (UD) combinations and 96 combinatorial dual (CD) combinations |
| Specialized Sample Types | Low-Input Samples, Not FFPE-Compatible | Not FFPE-Compatible | Blood, Not FFPE-Validated, Saliva |
| Species Details | Compatible with most large DNA genomes. | Compatible with most large DNA genomes. | Compatible with any species |
| Target Insert Size | 350 bp or 550 bp | 350 bp or 550 bp | ~350bp |
| Specification | TruSeq DNA Nano | TruSeq DNA PCR-Free |
|---|---|---|
| Kit type | TruSeq DNA Nano LT & HT | TruSeq DNA PCR-Free LT & HT |
| Description | Based upon widely adopted TruSeq library prep, with lower input and improved data quality | Superior genomic coverage with radically reduced library bias and gaps |
| Workflow | LT kits: Manual workflow
HT kits: Automation-friendly; manual protocols also available |
LT kits: Manual workflow
HT kits: Automation-friendly; manual protocols also available |
| Input quantity | 100–200 ng
|
1–2 μg |
| Includes PCR | Yes | No |
| Assay time | 5.5 hours | ~5 hours |
| Hands-on time | 4 hours | ~4 hours |
| Target insert size | 350 bp or 550 bp | 350 bp or 550 bp |
| Gel-free | Yes | Yes |
| Number of samples supported | 24 (LT) or 96 (HT) samples | 24 (LT) or 96 (HT) samples |
| Size-selection beads | Included | Included |
| Applications | Whole-genome sequencing applications, including whole-genome resequencing, de novo assembly, and metagenomics studies | |
| Sample multiplexing | Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations | |
| Compatible Illumina sequencers | MiniSeq, MiSeq, NextSeq, HiSeq, NovaSeq, HiSeq X, HiScanSQ, and Genome Analyzer systems | |
Data Sheet | PDF 11 MB
Mechanical DNA Fragmentation with the Q800R2 Sonicator
Technical Note | PDF 4 MB
Comparison of TruSeq Sample Preparation Kits
Technical Note | PDF < 1 MB
TruSeq Nano DNA Library Prep User-Supplied Consumables and Equipment Documentation
TruSeq DNA Nano Consumables & Equipment Documentation
TruSeq Nano DNA Library Prep Checklist Documentation
TruSeq HT Dual Index Quick Reference Card Documentation
Illumina Experiment Manager User Guide Documentation
TruSeq DNA Nano Reference Guide Documentation
TruSeq DNA Nano Checklist Documentation
TruSeq Nano DNA Library Prep Reference Guide Documentation
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Simple, all-inclusive whole-genome sequencing (WGS) library preparation that provides accurate and comprehensive coverage of complex genomes.
