AmpliSeq for Illumina Focus Panel
用于研究52个与实体瘤相关的基因的靶向DNA和RNA研究panel。Read More...
AmpliSeq for Illumina Focus Panel是一种靶向重测序分析套装，用于对52种与实体瘤具有已知关联的基因的生物标记物进行分析。利用这种Focus Panel，研究人员能够同时分析DNA和RNA。主要特点包括：
Focus Panel是集成工作流程的一部分，该工作流程包括基于AmpliSeq for Illumina聚合酶链式反应（PCR）的文库制备、采用Illumina边合成边测序（SBS）的新一代测序（NGS）技术以及自动化分析过程。
即用型panel助您节省鉴定目标基因、设计引物和优化panel所需的时间和精力。
| 仪器 | 推荐的样本数 | 读长 |
|---|---|---|
| iSeq 100 System | 8 samples per run (assumes minimum coverage of 500×) | 2 × 150 bp |
| MiniSeq System | Samples per run: mid output: 16, high output: 48 (assumes minimum coverage of 500×) | 2 × 150 bp |
| MiSeq System | Samples per run (by reagent kit version): v2 micro: 8, v2: 30, v3: 48 (assumes minimum coverage of 500×) | 2 × 150 bp |
| AmpliSeq for Illumina Focus Panel | AmpliSeq for Illumina Comprehensive Panel v3 | AmpliSeq for Illumina Cancer Hotspot Panel v2 | TruSight Tumor 15 | |
|---|---|---|---|---|
| Assay Time | 5-6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | 5-6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | |
| Cancer Type | Solid Tumor | Solid Tumor | Solid Tumor, 泛癌种 | Solid Tumor |
| Content Specifications | DNA and RNA targets for 52 oncogenes | DNA and RNA targets for 161 oncogenes | Hotspot regions of 50 genes with known associations to cancer | |
| Description | Somatic analysis research on 52 genes associated with solid tumor cancers. | Somatic analysis research on hotspot and full-length targets of genes associated with solid tumor cancers. | Somatic analysis research into hotspot regions of 50 cancer-related genes. | |
| Hands-On Time | < 1.5 hours | < 1.5 hours | < 1.5 hours | |
| Input Quantity | 1–100 ng (10 ng recommended per pool) | 1–100 ng (10 ng recommended per pool) | 1–100 ng (10 ng recommended per pool) | |
| Method | Amplicon Sequencing , 靶向DNA测序 , 靶向RNA测序 | Amplicon Sequencing , 靶向DNA测序 , 靶向RNA测序 | Amplicon Sequencing , 靶向DNA测序 | Amplicon Sequencing , 靶向DNA测序 |
| Multiplexing | 96 dual index combinations | 96 dual index combinations | 96 dual index combinations | |
| Nucleic Acid Type | DNA, RNA | DNA, RNA | DNA | DNA |
| Specialized Sample Types | FFPE Tissue | Blood, FFPE Tissue | Blood, FFPE Tissue | FFPE Tissue |
| Species Category | 人类 | 人类 | 人类 | 人类 |
AmpliSeq for Illumina Oncology Panels on the iSeq 100 System
Application Note | PDF< 1 MB
Analytical performance of the AmpliSeq for Illumina Focus Panel with FFPE samples
Application Note | PDF< 1 MB
Automated Solutions for AmpliSeq for Illumina Sequencing Panels
Application Note | HTML
Sample Identification with the AmpliSeq for Illumina Sample ID Panel Application Note
Application Note | HTML
Data Sheet | HTML | 5 versions
AmpliSeq for Illumina Focus Panel Gene List
product_file | EXCEL< 1 MB
AmpliSeq for Illumina Focus Panel Support - Documentation & Literature
AmpliSeq for Illumina Focus Panel Reference Guide Documentation
Illumina Adapter Sequences Document Documentation
Index Adapters Pooling Guide Documentation
AmpliSeq for Illumina Direct FFPE DNA Kit Reference Guide Documentation
AmpliSeq for Illumina Focus Panel Consumables & Equipment Documentation
AmpliSeq for Illumina Focus Panel Checklist Documentation
自定义协议选择器
Generates customized, end-to-end instructions
Targeted research panel investigating hotspot regions of 50 genes with known associations to cancer.
Targeted DNA and RNA research panel investigating variants across 161 genes associated with a range of cancer types.
