AmpliSeq for Illumina BRCA Panel

用于研究BRCA1BRCA2基因的体细胞和生殖系变异的靶向研究panel。 阅读更多...
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Panel

AmpliSeq™ BRCA Panel for Illumina®

20019168


Library Prep

AmpliSeq™ Library PLUS (24 Reactions) for Illumina®

20019101

AmpliSeq™ Library PLUS (96 Reactions) for Illumina®

20019102

AmpliSeq™ Library PLUS (384 Reactions) for Illumina®

20019103


Index Adapters

AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)

20019104

AmpliSeq™ CD Indexes Set A for Illumina®

20019105

AmpliSeq™ CD Indexes Set B for Illumina®

20019106

AmpliSeq™ CD Indexes Set C for Illumina®

20019107

AmpliSeq™ CD Indexes Set D for Illumina®

20019167

AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)

20031676

配件产品

AmpliSeq™ for Illumina® Direct FFPE DNA

20023378

AmpliSeq™ Library Equalizer for Illumina®

20019171

产品特色

The AmpliSeq for Illumina BRCA Panel is a targeted resequencing assay for research of somatic and germline variants across BRCA1 and BRCA2 genes. Key features include:

Relevant Gene Content
  • Target all exonic regions and flanking intronic sequences of BRCA1 and BRCA2
Fast, Streamlined Workflow
  • Prepare sequencing-ready libraries in a single day from as little as 1 ng of high quality or 10 ng of formalin-fixed, paraffin-embedded (FFPE) input samples
Accurate Data
  • Detect somatic mutations as low as 5% variant allele frequency using local or cloud based analysis

The BRCA Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.

BRCA1 and BRCA2 are human tumor suppressor genes that, when carrying specific mutations, have been implicated in an increased risk for breast and ovarian cancers. This ready-to-use panel saves researchers the time and effort of identifying targets, designing primers, and optimizing panels.

Learn More About AmpliSeq for Illumina

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规格

计划推荐

仪器 推荐的样本数 读长
iSeq 100 System 12 samples per run (assumes minimum coverage of 500×)
96 samples per run (assumes minimum coverage of 50x)
2 × 150 bp
2 × 150 bp
MiniSeq System Samples per run: mid output: 24, high output: 80 (assumes minimum coverage of 500×) 2 × 150 bp
MiSeq System Samples per run at 500x minimum coverage (by reagent kit version): v2 nano: 3, v2 micro: 12, v2: 48, v3: 80
Samples per run at 50x minimum coverage (by reagent kit version): v2 nano: 32, v2 micro: 96
2 × 150 bp
2 × 150 bp

产品比较

AmpliSeq for Illumina BRCA Panel TruSight Cancer癌症相关基因探针集
内容说明 BRCA1BRCA2的外显子区域和侧翼内含子序列 固定的探针集富集了与常见和罕见癌症相关的94个基因和284个SNP。
分析时间 5小时(仅包括文库制备时间;不包括文库定量、归一化或混合时间) 1.5天
变异种类 体细胞变异, 生殖系变异, 插入缺失 (indels), 单核苷酸多态性 (SNPs) 生殖系变异
多重分析 96个双标签序列组合 多达96重
手动操作时间 <1.5小时 5小时
方法 扩增子测序, 靶向DNA测序 靶向富集, 靶向DNA测序
物种类别 人类 人类
特殊的样本类型 血液, FFPE组织 Not FFPE-Compatible
癌症类型 实体瘤 泛癌种
起始量 1–100 ng(建议每个混池10 ng) 50 ng DNA

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TruSight Cancer癌症相关基因探针集

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