AmpliSeq for Illumina BRCA Panel
用于研究BRCA1和BRCA2基因的体细胞和生殖系变异的靶向研究panel。
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The AmpliSeq for Illumina BRCA Panel is a targeted resequencing assay for research of somatic and germline variants across BRCA1 and BRCA2 genes. Key features include:
The BRCA Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.
BRCA1 and BRCA2 are human tumor suppressor genes that, when carrying specific mutations, have been implicated in an increased risk for breast and ovarian cancers. This ready-to-use panel saves researchers the time and effort of identifying targets, designing primers, and optimizing panels.
| 仪器 | 推荐的样本数 | 读长 |
|---|---|---|
| iSeq 100 System | 12 samples per run (assumes minimum coverage of 500×) 96 samples per run (assumes minimum coverage of 50x) |
2 × 150 bp 2 × 150 bp |
| MiniSeq System | Samples per run: mid output: 24, high output: 80 (assumes minimum coverage of 500×) | 2 × 150 bp |
| MiSeq System | Samples per run at 500x minimum coverage (by reagent kit version): v2 nano: 3, v2 micro: 12, v2: 48, v3: 80 Samples per run at 50x minimum coverage (by reagent kit version): v2 nano: 32, v2 micro: 96 |
2 × 150 bp 2 × 150 bp |
| AmpliSeq for Illumina BRCA Panel | TruSight Cancer癌症相关基因探针集 | |
|---|---|---|
| 内容说明 | BRCA1和BRCA2的外显子区域和侧翼内含子序列 | 固定的探针集富集了与常见和罕见癌症相关的94个基因和284个SNP。 |
| 分析时间 | 5小时（仅包括文库制备时间；不包括文库定量、归一化或混合时间） | 1.5天 |
| 变异种类 | 体细胞变异, 生殖系变异, 插入缺失 (indels), 单核苷酸多态性 (SNPs) | 生殖系变异 |
| 多重分析 | 96个双标签序列组合 | 多达96重 |
| 手动操作时间 | ＜1.5小时 | 5小时 |
| 方法 | 扩增子测序, 靶向DNA测序 | 靶向富集, 靶向DNA测序 |
| 物种类别 | 人类 | 人类 |
| 特殊的样本类型 | 血液, FFPE组织 | Not FFPE-Compatible |
| 癌症类型 | 实体瘤 | 泛癌种 |
| 起始量 | 1–100 ng（建议每个混池10 ng） | 50 ng DNA |
AmpliSeq for Illumina Oncology Panels on the iSeq 100 System
Application Note | PDF < 1 MB
Automated Solutions for AmpliSeq for Illumina Sequencing Panels
Application Note | HTML
AmpliSeq for Illumina BRCA Panel Data Sheet
Data Sheet | HTML
Sample identification with the AmpliSeq for Illumina Sample ID Panel
Application Note | PDF < 1 MB
AmpliSeq for Illumina BRCA Panel Reference Guide Documentation
AmpliSeq for Illumina BRCA Panel Consumables & Equipment Documentation
Illumina Adapter Sequences Documentation
AmpliSeq for Illumina BRCA Panel Checklist Documentation
AmpliSeq for Illumina BRCA Panel Support - Documentation & Literature
AmpliSeq for Illumina Custom and Community Panels Documentation
Index Adapters Pooling Guide Documentation
AmpliSeq for Illumina Direct FFPE DNA Kit Reference Guide Documentation
自定义协议选择器
Generates customized, end-to-end instructions
