小型全基因组测序简介
小型基因组测序（≤ 5 Mb）涉及对细菌、病毒或其他微生物的整个基因组进行测序，并将序列与已知参考序列进行比较。小型微生物基因组的测序对公共卫生领域的食品检测、传染性疾病监控、分子流行病学研究和环境宏基因组学很有用。
小型基因组测序的优势
- 研究培养的单个生物的所有基因
- 同时对数千种生物体进行测序
- 对微生物或病毒基因组进行全面分析
- 提供同源染色体的不同基因信息，协助发现微生物或病毒样本中的新生物标记，为单倍型分析提供支持
快速且无需培养的微生物分析
与传统方法不同，利用新一代测序（NGS）的小型基因组测序研究不依靠工作量大的克隆步骤。NGS也允许生物学家通过多重分析同时测序数百个生物体。NGS能鉴定低频变异、基因组重排和其他遗传改变，而其他方法可能会漏检或者鉴定成本过高。短短2天内即可制备小型基因组的DNA文库，并对其进行测序和分析。
Shortening the Journey to Diagnosis
Whole genome sequencing may be the key to helping parents avoid months or years of inconclusive tests. Listen to experts from the Undiagnosed Diseases Network to learn more.
Listen to the Podcast推荐的小型全基因组测序工作流程
文库制备
测序
数据分析
Illumina Sequencers Help Characterize and Control Coronavirus
NGS, by rapidly characterizing the full viral genome, plays a critical role in the outbreak of emerging viruses by enabling phylogenetic characterization without a prior knowledge of the potential family. This detailed viral information is enabling public health officials to respond with unprecedented speed and breadth.
Read Article
全面的小型全基因组测序工作流程
Illumina边合成边测序（SBS）技术是最广泛采用的NGS技术，产生了全世界约90%的测序数据。*
除了业界领先的数据质量，Illumina还提供集成的工作流程，简化了从文库制备到数据分析的小型基因组测序过程。
Whole-Genome Sequencing and Drug Discovery
Learn how Joseph Petrosino, PhD uses whole-genome shotgun sequencing to obtain functional gene pathway and strain-based information in microbiome studies. These methods provide researchers and pharmaceutical companies with data to refine drug discovery and development.
Read ArticleSequencing Method Explorer
Use this interactive tool to explore experimental next-generation sequencing (NGS) library preparation methods compiled from the scientific literature. New methods will be continuously added.
Find a Method
相关解决方案
鸟枪法宏基因组学
鸟枪法宏基因组测序技术能对给定的复杂样本中存在的所有生物体的所有基因进行全面的分析。它能让研究人员检测极低丰度的微生物，而利用其他方法可能无法识别这些微生物或者价格太高。深入了解鸟枪法宏基因组测序。
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Additional Resources
Tracking the Ebola Virus
Harvard researchers used sequencing to study Ebola outbreaks and understand viral mutations.
Small Genome Sequencing With the NextSeq Series
The NextSeq Series of sequencing systems offers an accessible whole-genome sequencing solution.
葡萄酒和奶酪的微生物组研究
David Mills博士对与葡萄酒和奶酪生产有关的微生物进行了测序，以此来了解它们对风味的影响。
Single-Cell Sequencing of Marine Microbes
Dr. Ramunas Stepanauskas uses single-cell sequencing to characterize bacteria inhabiting the ocean.
Sequencing Tracks a Deadly Porcine Virus
Dr. Ganwu Li and other veterinary scientists use sequencing to study a virus that threatens the pork industry.
Nextera DNA Flex on the iSeq 100 System
View tips on transitioning to Nextera DNA Flex on the iSeq 100 System, including considerations for viral and bacterial sequencing.
*根据Illumina公司2015年存档数据计算