Ribo-Seq/ART-Seq/GTI-Seq

Ribo-Seq/ART-Seq/GTI-Seq

Active mRNA Translation Sequencing (ART-seq), also called ribosome profiling (Ribo-Seq ) or Global Translation Initiation Sequencing (GTI-Seq ), isolates RNA that is being processed by the ribosome in order to monitor the translation process. In this method ribosome-bound RNA first undergoes digestion. The RNA is then extracted and the rRNA is depleted. Extracted RNA is reverse-transcribed to cDNA. Deep sequencing of the cDNA provides the sequences of RNAs bound by ribosomes during translation.

• ART-Seq/Ribo-Seq: Ingolia N. T., Ghaemmaghami S., Newman J. R. and Weissman J. S. (2009) Genome-wide analysis in vivo of translation with nucleotide resolution using ribosome profiling. Science 324: 218-223
• GTI-Seq: Wan J. and Qian S. B. (2014) TISdb: a database for alternative translation initiation in mammalian cells. Nucleic Acids Res 42: D845-850

1. Crappe J., Ndah E., Koch A., Steyaert S., Gawron D., et al. (2015) PROTEOFORMER: deep proteome coverage through ribosome profiling and MS integration. Nucleic Acids Res 43: e29
2. Gao X., Wan J., Liu B., Ma M., Shen B., et al. (2015) Quantitative profiling of initiating ribosomes in vivo. Nat Methods 12: 147-153
3. Han Y., Gao X., Liu B., Wan J., Zhang X., et al. (2014) Ribosome profiling reveals sequence-independent post-initiation pausing as a signature of translation. Cell Res 24: 842-851
4. Han P., Li W., Lin C. H., Yang J., Shang C., et al. (2014) A long noncoding RNA protects the heart from pathological hypertrophy. Nature 514: 102-106