4-C/4C-Seq

4-C/4C-Seq

Circular chromatin conformation capture (4-C), also called 4C-Seq, is a method similar to 3-C and is sometimes called circular 3C. It allows the unbiased detection of all genomic regions that interact with a particular region of interest . In this method, DNA-protein complexes are crosslinked using formaldehyde. The sample is fragmented, and the DNA is ligated and digested. The resulting DNA fragments self-circularize, followed by reverse PCR and sequencing. Deep sequencing provides base-pair resolution of ligated fragments.

• 4C-Seq: Zhao Z., Tavoosidana G., Sjolinder M., Gondor A., Mariano P., et al. (2006) Circular chromosome conformation capture (4C) uncovers extensive networks of epigenetically regulated intra- and interchromosomal interactions. Nat Genet 38: 1341-1347

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