TRAP-Seq
TRAP-Seq
Targeted purification of polysomal mRNA (TRAP-Seq) maps translating mRNAs under various conditions. In this method, tagged ribosomal proteins are expressed in cells. The tagged ribosomal proteins are then purified and the RNA isolated. RNAs are reverse-transcribed to cDNA. Deep sequencing of the cDNA provides single-base resolution of translating RNA.
Pros:
- Allows detection of translating RNAs
- RNAs translated by specific targeted ribosomes can be assessed
- No prior knowledge of the RNA is required
- Genome-wide RNA screen
Cons:
- Not as specific as more recently developed methods, such as Ribo-Seq