TIVA
TIVA
Transcriptome in vivo analysis (TIVA) is a protocol that captures mRNA from live cells. In this method, a TIVA tag is loaded into cells. Photoactivation cleaves the photocleaveable linkers, allowing it to hybridize to mRNA. The biotin bound mRNA is captured using streptavidin coated magnetic beads, transcribed to cDNA and sequenced. Sequencing the cDNA provides transcriptome analysis of RNA from single cells from complex tissues.
- TIVA: Lovatt D., Ruble B. K., Lee J., Dueck H., Kim T. K., et al. (2014) Transcriptome in vivo analysis (TIVA) of spatially defined single cells in live tissue. Nat Methods 11: 190-196