NET-Seq
NET-Seq
Native elongating transcript sequencing (NET-Seq) maps transcription through the capture of 3’ RNA . In this method the RNA polymerase II elongation complex is immunoprecipitated, and RNA is extracted and reverse-transcribed to cDNA. Deep sequencing of the cDNA allows for 3’-end sequencing of nascent RNA, providing nucleotide resolution at transcription.
- NET-Seq: Churchman L. S. and Weissman J. S. (2011) Nascent transcript sequencing visualizes transcription at nucleotide resolution. Nature 469: 368-373
- Mayer A., di Iulio J., Maleri S., Eser U., Vierstra J., et al. (2015) Native elongating transcript sequencing reveals human transcriptional activity at nucleotide resolution. Cell 161: 541-554
- Nojima T., Gomes T., Grosso A. R., Kimura H., Dye M. J., et al. (2015) Mammalian NET-Seq Reveals Genome-wide Nascent Transcription Coupled to RNA Processing. Cell 161: 526-540
- Marquardt S., Escalante-Chong R., Pho N., Wang J., Churchman L. S., et al. (2014) A chromatin-based mechanism for limiting divergent noncoding transcription. Cell 157: 1712-1723
- Weber C. M., Ramachandran S. and Henikoff S. (2014) Nucleosomes are context-specific, H2A.Z-modulated barriers to RNA polymerase. Mol Cell 53: 819-830