CEL-Seq
CEL-Seq
Cell expression by linear amplification sequencing (CEL-Seq) is a method that utilizes barcoding and pooling of RNA to overcome challenges from low input. In this method, each cell undergoes reverse transcription with a unique barcoded primer in its individual tube. After second-strand synthesis, cDNAs from all reaction tubes are pooled, and PCR-amplified. Paired-end deep sequencing of the PCR products allows for accurate detection of sequence derived from sequencing both strands.
- CEL-Seq: Hashimshony T., Wagner F., Sher N. and Yanai I. (2012) CEL-Seq: single-cell RNA-Seq by multiplexed linear amplification. Cell Rep 2: 666-673