CAGE-Seq
CAGE-Seq
Cap analysis gene expression sequencing (CAGE) is a protocol that can measure RNA expression and map transcription start sites (TSS) in promoters. In this method, RNA is first reverse transcribed using a random primer. RNA cap and 3’-ends are biotinylated. Non-hybridized single-stranded RNAs are digested with RNase, leaving 5’ complete cDNAs which are captured using streptavidin beads. The cDNA is then processed for sequencing.
- CAGE-Seq: Takahashi H., Lassmann T., Murata M. and Carninci P. (2012) 5' end-centered expression profiling using cap-analysis gene expression and next-generation sequencing. Nat Protoc 7: 542-561
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- Fort A., Hashimoto K., Yamada D., Salimullah M., Keya C. A., et al. (2014) Deep transcriptome profiling of mammalian stem cells supports a regulatory role for retrotransposons in pluripotency maintenance. Nat Genet 46: 558-566