Comparison Table
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| TruSeq Custom Amplicon v1.5 | TruSeq Custom Amplicon Low Input | Nextera Rapid Capture Custom Enrichment | Nextera XT DNA | |
|---|---|---|---|---|
| 描述 | Fully customizable multiplexed amplicon assay used for any application with 50ng input requirement or higher. | Fully customizable multiplexed amplicon assay used for low input and/or FFPE applications. Cannot be used with applications requiring long amplicons of 425bp. | 定制流程实现了靶向基因组区域的富集。设计完全定制的集合，或在Illumina现有的集合或之前订购的定制产品上添加。 | 在90分钟内制备测序即用的文库，而手工操作时间仅为15分钟，且DNA样品量少。 |
| 设计 | Customers use Illumina DesignStudio software to design probes targeting their regions of interest. | Customers use Illumina DesignStudio software to design probes targeting their regions of interest. | 利用DesignStudio软件，用户设计人类基因组的定制探针 | 独立设计的客户探针，靶定长度至少为300 bp的扩增子。 |
| 内容范围 | 4–650 kb | 4–650 kb | 0.5–15Mb | 300bp–20Mb |
| Kit Size | 96 samples | 16 or 96 samples | 48, 96, or 288 samples | 24 or 96 samples |
| 起始量 | 50 ng DNA | 10 ng DNA | 50 ng DNA | 1 ng DNA |
| 多重分析 | 1–96 | 1–96 | 1–96 | 1–384 |
| 方法 | 扩增子 | 扩增子 | 富集 | 扩增子 |
| FFPE 兼容性 | 兼容 | 兼容 | 不兼容 | 不兼容 |