TruSeq Rapid Exome

TruSeq Rapid Exome

The most efficient Exome workflow focused on 45 Mb of coding sequence from >98% of RefSeq, CCDS, and Ensembl coding content.

Catalog ID(s): # of Index Combinations Pre-Enrichment Multiplexing Enrichment Rxns Per Kit Maximum Samples Per Kit
FC-144-1000 1 1 8 8
FC-144-1001 3 3 8 24
FC-144-1002 6 6 8 48
FC-144-1003 9 9 8 72
FC-144-1004 24 12 8 96

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* Library prep is compatible with all Illumina instruments, except the X5 and X10 systems. Recommended instruments chosen based on typical output and analysis need for selected application.
† 50M Reads, 50x Coverage, 4GB of Data

Project Recommendations
Species Compatibility Human
Recommended Illumina Instrument* NextSeq 500 HiSeq 2500 (Dual Flow Cell)
Recommended Samples per run 3 12 24 156
Run Mode Mid Output High Output Rapid Run High Output
Max Recommended Read Length 2×75 bp 2×75 bp 2×75 bp 2×75 bp
Recommended Secondary Analysis
  • BWA Enrichment BaseSpace App
  • Isaac Enrichment v2 BaseSpace App
Recommended Down Stream Analysis VariantStudio