Resources for the NovaSeq 6000 System
System Literature
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Illumina Proactive Service connectivity guide - NovaSeq 6000Brochure | PDF | < 1 MB
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De Novo Genome Assembly with Illumina Sequencing and the NRGene DeNovoMAGIC SolutionApplication note | HTML |
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Human Whole-Genome Sequencing with the NovaSeq 6000 Sequencing SystemApplication note | PDF | < 1 MB
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NovaSeq 6000 System Quality Scores and RTA3 SoftwareApplication note | PDF | < 1 MB
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QC and rebalancing of single-cell gene expression libraries using the iSeq™ 100 SystemApplication note | PDF | 2 MB
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Sequencing Library QC on the iSeq SystemApplication note | PDF | < 1 MB
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Enhanced sequencing capabilities with the NovaSeq™ 6000 v1.5 Reagent KitTechnical note | PDF | < 1 MB
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The NovaSeq Xp workflow provides flexibility and control without sacrificing data quality or yieldTechnical note | PDF | < 1 MB
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The NovaSeq 6000 System Specification SheetData sheet | HTML |
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Brochure | PDF | 8 versions
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An automated Nextera DNA Flex library preparation workflow for high-throughput metagenomicsApplication note | PDF | < 1 MB
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High-performance whole-genome sequencing with Illumina DNA PCR-Free Prep, TagmentationApplication note | PDF | < 1 MB
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High-resolution, high-throughput spatial transcriptomics of complex tissuesApplication note | PDF | 3 MB
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Long read lengths for shotgun metagenomicsApplication note | PDF | < 1 MB
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Ultra-long range phasing with linked-read sequencing technologyApplication note | PDF | 1 MB
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Technical note | PDF | 2 versions
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Explore the transcriptome with single-cell resolutionTechnical note | PDF | 1 MB
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Impact of NovaSeq flow cell lane edge integrity on sequencing performanceTechnical note | PDF | 1 MB
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Multiomic interrogation of the immune system at single-cell resolutionTechnical note | PDF | 1 MB
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Population genetics data processing with the DRAGEN Bio-IT PlatformTechnical note | PDF | 1 MB
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Technical note | PDF | 2 versions
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Unify single-cell gene expression and chromatin accessibilityTechnical note | PDF | 1 MB
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Data sheet | PDF | 7 versions
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High-plex spatial proteogenomics of FFPE tissue sectionsApplication note | PDF | 2 MB
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Technical note | PDF | 11 versions
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Sequencing performance with NovaSeq Control Software v1.8Technical note | PDF | < 1 MB
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Technical note | PDF | 9 versions
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Application note | PDF | 7 versions
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Illumina DNA PrepData sheet | PDF | < 1 MB
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NovaSeq 6000 Sequencing SystemData sheet | PDF | < 1 MB
Product Selection and Experiment Planning Tools
Find tools to help you choose the right products for your needs and plan your experiments. Identify the right library prep kit or microarray, calculate sequencing coverage, explore methods compiled from publications, design custom assays, and more.
Explore Tools
Education and Support Resources
NovaSeq 6000 Manuals and Documents
Find the system guide, site prep guide, indexed sequencing guide, and other supporting documentation.
Sequencing Online Training
Online Illumina sequencing courses are free, interactive, and available any time.
Instrument Buying Options
Don’t let your budget hold you back. Explore different buying options to find which best fits your needs.
Custom Protocol Selector
Generate your own customized documentation, specifically tailored to your experiment.
Quality Scores
Additional information about sequencing quality scores, how they are calculated, and the relationship between sequencing quality score and base call accuracy.
View Sequencing Coverage Tips
Learn how to estimate and achieve the necessary sequencing coverage for your experiment.
Human WGS on the NovaSeq 6000 System
See our best practices for generating high-quality data from human whole-genome sequencing on the NovaSeq 6000 System.