Advanced chemistry, optics, and informatics combine to deliver exceptional sequencing speed and data quality, outstanding throughput, and scalability.
Library preparation is a required sequencing step. Use our selection tool to determine the best library prep kit for your needs based on your application, method, and more.
Identify the best approach, manage your workflow, purchase what you need, and get support.
Get up and running quickly with a rental or leasing program designed to work with your budget.
NovaSeq X Sequencing System
20084803
Includes one NovaSeq X Sequencing System with embedded touch screen monitor and on-instrument computer, control software, hardware, integrated DRAGEN secondary analysis pipelines and lossless data compression, installation and training, and 12 months warranty (including parts and labor) to support automated generation of DNA clonal clusters by bridge amplification, sequencing, and primary and secondary analysis. Qualification services, instrument service plans, and reagent kits sold separately.
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NovaSeq X Plus Sequencing System
20084804
Includes one NovaSeq X Plus Sequencing System with embedded touch screen monitor and on-instrument computer, control software, hardware, integrated DRAGEN secondary analysis pipelines, installation and training, and 12 months warranty (including parts and labor) to support automated generation of DNA clonal clusters by bridge amplification, sequencing, and primary and secondary analysis. Qualification services, instrument service plans, and reagent kits sold separately.
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NovaSeq™ X Series 25B Reagent Kit (300 Cyc)
20104706
This reagent kit provides one NovaSeq X Series 25B flow cell (with 8- lanes) and reagent consumables to support a single flow cell 300 cycles run on the NovaSeq X and NovaSeq X Plus systems.
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NovaSeq™ X Series 25B Reagent Kit (200 Cyc)
20125968
This reagent kit provides one NovaSeq X Series 25B flow cell (with 8- lanes) and reagent consumables to support a single flow cell 200 cycles run on the NovaSeq X and NovaSeq X Plus systems. Please note that the lyo insert provided with this kit is compatible only with NovaSeq X 25B 100/200c kits 9MN 20125967 and 20125968) and not compatible with NovaSeq X Series 25B Reagent Kit 300c (MN 20104705).
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NovaSeq™ X Series 25B Reagent Kit (100 Cyc)
20125967
This reagent kit provides one NovaSeq X Series 25B flow cell (with 8- lanes) and reagent consumables to support a single flow cell 100 cycles run on the NovaSeq X and NovaSeq X Plus systems. Please note that the lyo insert provided with this kit is compatible only with NovaSeq X 25B 100/200c kits 9MN 20125967 and 20125968) and not compatible with NovaSeq X Series 25B Reagent Kit 300c (MN 20104705).
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NovaSeq X Series 10B Reagent Kit (300 Cycle)
20085594
This reagent kit provides one NovaSeq X Series 10B flow cell (with 8- lanes) and reagent consumables to support a single flow cell 300 cycles run on the NovaSeq X and NovaSeq X Plus systems.
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NovaSeq X Series 10B Reagent Kit (200 Cycle)
20085595
This reagent kit provides one NovaSeq X Series 10B flow cell (with 8- lanes) and reagent consumables to support a single flow cell 200 cycles run on the NovaSeq X and NovaSeq X Plus systems.
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NovaSeq X Series 10B Reagent Kit (100 Cycle)
20085596
This reagent kit provides one NovaSeq X Series 10B flow cell (with 8- lanes) and reagent consumables to support a single flow cell 100 cycles run on the NovaSeq X and NovaSeq X Plus systems.
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NovaSeq™ X Series 1.5B Reagent Kit (300 Cyc)
20104705
This reagent kit provides one NovaSeq X Series 1.5B flow cell (with 2- lanes) and reagent consumables to support a single flow cell 300 cycles run on the NovaSeq X and NovaSeq X Plus systems.
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NovaSeq™ X Series 1.5B Reagent Kit (200 Cyc)
20104704
This reagent kit provides one NovaSeq X Series 1.5B flow cell (with 2- lanes) and reagent consumables to support a single flow cell 200 cycles run on the NovaSeq X and NovaSeq X Plus systems.
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NovaSeq™ X Series 1.5B Reagent Kit (100 Cyc)
20104703
This reagent kit provides one NovaSeq X Series 1.5B flow cell (with 2- lanes) with reagent consumables to support a single flow cell 100 cycles run on the NovaSeq X and NovaSeq X Plus systems.
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NovaSeq™ X Series Custom-Primer Buffer
20100055
Buffer used for the custom primer workflow on the NovaSeq X Series. This kit is sufficient for 1 custom primer workflow for a single flow cell sequencing run.
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NOVASEQ™X Series Library Tube Strip (10B/25B)
20101913
NOVASEQ™X Series Library Tube Strip compatible with a NovaSeq X Series 10B Reagent Kit or a NovaSeq X Series 25B Reagent Kit. This part contains one 10B/25B (8-lane) library tube strip.
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NOVASEQ™X Series Library Tube Strip (1.5B)
20072272
NOVASEQ™ X Series Library Tube Strip compatible with a NovaSeq X Series 1.5B Reagent Kit. This part contains one 1.5B (2-lane) library tube strip.
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NOVASEQ™X Series Wash Flow Cell
20088067
NOVASEQ™X Series Wash Flow Cell is used during washes on the NovaSeq X or NovaSeq X Plus system. This part contains one wash flow cell.
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NOVASEQ™X Series Air Filter
20073109
Replacement NovaSeq X Series Air Filter for the NovaSeq X or NovaSeq X Plus system. This part contains one air filter.
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NovaSeq X Series Reagent Kits are required to use the NovaSeq X and NovaSeq X Plus Systems. You’ll also need a library prep kit. In addition, PhiX Control v3 is recommended for use as a sequencing control. The custom-primer buffer is required for utilizing the custom primer workflow.
With the NovaSeq X Series, Illumina continues to set the standard for accuracy and usability. Advanced chemistry, optics, and informatics combine to deliver exceptional speed and data quality. Users can have outstanding throughput and scalability without sacrificing flexible, streamlined, and easy-to-use workflows. Learn how the NovaSeq X Series can be a game-changer for your projects.
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The NovaSeq X Series offers the throughput, accuracy, and cost savings needed to enable more data intensive applications and deliver meaningful insights at scale. Sequencing on the NovaSeq X Series reduces the cost per gigabase (Gb) by up to 60% compared to the NovaSeq 6000 system. Powered by XLEAP-SBS chemistry, the NovaSeq X Series delivers the high-quality data that NGS customers expect with best-in-class secondary analysis.
Explore additional NovaSeq X Series features and discover the enormous breadth of supported applications and methods.
Watch the NovaSeq X Series: One flow cell, multiple omes webinar and discover how it supports methods for CRISPR, single cell, circulation RNA, and more.
The NovaSeq X System features a single flow cell configuration with an output of up to 8 terabases (Tb), or up to 26 billion single reads per run. The NovaSeq X Plus System offers up to 16 Tb output, or up to 52 billion single reads, per dual flow cell run. To ensure future scalability, customers who purchase a NovaSeq X System can fully upgrade to the NovaSeq X Plus System for dual flow cell capability as needs evolve.
Yes, the NovaSeq X and NovaSeq X Plus Systems incorporate patterned flow cell technology to generate an ultrahigh level of throughput for a broad range of sequencing applications.
For NovaSeq X Series 1.5B Reagent Kits, strictly adhere to prescribed thaw conditions and timings in the System Guide. Do not rely solely on visual checks to confirm reagents have thawed. For NovaSeq X Series 100c and 200c 25B Reagent Kits, ensure you have updated to instrument software v1.3 or newer.
With up to eight independent lanes per flow cell, NovaSeq X Series flow cells streamline operations by enabling automatic loading of up to eight separate library pools per flow cell—without the need for an accessory kit. The NovaSeq X Series 10B Flow Cell and NovaSeq X Series 25B Flow Cell have 8 lanes for sequencing pooled libraries. The NovaSeq X Series 1.5B Flow Cell has two lanes for sequencing pooled libraries.
Visit our NovaSeq X Series Reagent Kits page for additional information.
Advanced chemistry, optics, and informatics combine to deliver exceptional sequencing speed and data quality, outstanding throughput, and scalability.
Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.
Enable CGP with a large pan-cancer panel covering all main variant classes plus key biomarkers (TMB, MSI, and HRD) from FFPE tissue.
A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.
Reagent kits for the NovaSeq X Series include a reagent cartridge, buffer cartridge, flow cell, lyo insert, pre-load buffer, and library tube strip.
Enable CGP with a pan-cancer panel capable of detecting key IO gene signatures (TMB, MSI) plus all main variant classes from ctDNA in blood plasma.
Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI) from FFPE tissue, with added scalability.
Simple, all-inclusive whole-genome sequencing (WGS) library preparation that provides accurate and comprehensive coverage of complex genomes.
A complete targeted resequencing solution for a wide range of applications, with fast integrated workflow and options for custom and fixed enrichment panels.
Streamlined RNA-Seq solution for clear and comprehensive analysis across the coding and non-coding transcriptome with exceptional study flexibility.
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