AmpliSeq for Illumina Comprehensive Panel v3
Targeted DNA and RNA research panel investigating variants across 161 genes associated with a range of cancer types.
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Panel
Library Prep
Index Adapters
Accessory Products
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Product Highlights
The AmpliSeq for Illumina Comprehensive Panel v3 offers coverage of key cancer genes, including kinase domains and genes involved in DNA repair.
Relevant Gene Content
- Target 161 unique cancer-associated genes
Fast, Streamlined Workflow
- Prepare sequencing-ready libraries in a single day from as little as 1 ng of high quality or 10 ng of formalin-fixed, paraffin-embedded (FFPE) input samples
Accurate Data
- Detect somatic mutations down to 5% frequency using local or cloud-based analysis
The Comprehensive Panel v3 is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.
The panel content spans hotspot regions, full-length genes*, copy number genes, and inter- and intragenic gene fusions. This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.
*For this panel, Illumina defines full-length gene content as
covering the majority of the gene’s coding regions, generally having > 90% in silico
coverage and with most genes having > 99% coverage. Please refer to the provided product manifest file for further detail.
Frequently Purchased Together
Specifications
Project Recommendations
| Instrument | Recommended Number of Samples | Read Length |
|---|---|---|
| MiniSeq System | 3 samples per run (high output; assumes minimum coverage of 500×) | 2 × 150 bp |
| MiSeq System | 3 samples per run with v3 reagents (assumes minimum coverage of 500×) | 2 × 150 bp |
| NextSeq 550 System | Samples per run: mid output: 16, high output: 48 (assumes minimum coverage of 500×) | 2 × 150 bp |
Product Comparison
| AmpliSeq for Illumina Comprehensive Panel v3 | TruSight Tumor 170 | TruSight Tumor 15 | AmpliSeq for Illumina Focus Panel | AmpliSeq for Illumina Cancer Hotspot Panel v2 |
|---|
Product Literature
Automated Solutions for AmpliSeq for Illumina Sequencing Panels
Application note | HTML
Data sheet | HTML | 5 versions
Ampliseq for Illumina Comprehensive Panel v3 Gene List
product_file | EXCEL < 1 MB
NextSeq 550 amplicon sequencing solution
Application note | PDF < 1 MB
A dedicated support section is not currently available for this product
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