Targeted methylation sequencing (Methyl-Seq) offers a balanced, cost-effective choice between whole-genome bisulfite sequencing and methylation arrays that can support both screening and biomarker discovery study objectives.1 TruSeq Methyl Capture EPIC Library Prep supports researchers seeking a deeper understanding of the role of methylation in gene regulation.
Comprehensive methylation-site content: Offers significantly higher coverage of emerging epigenetic regions of interest compared to other Methyl-Seq kits currently on the market (See Supporting Data section, Epigenetic Regions Covered).
Maximize discovery power, minimize cost: Efficiently query differentially methylated bases to conserve research dollars.
Convenient kit configuration: Includes bisulfite conversion, library prep, target enrichment, and purification reagents in a single kit for hassle-free ordering.
User-friendly data analysis: Analyze sequence data in BaseSpace Sequence Hub with methylation apps designed for biologists.
仪器 | 推荐的样本数 | 读长 |
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NextSeq 550 System | 每次运行8个样本(高通量;基于40倍平均覆盖度和90%以上的目标碱基覆盖度≥10倍) | 2 x 100 bp (最大推荐值) |
NovaSeq 6000 System | 每次运行的样本(双流动槽):S1:48,S2:48,S4:96(受可用标签组合的限制;基于>5500万条read) | 2 × 100 bp (最大推荐值) |
HiSeq 2500 System | 每次运行的样本:快速运行:8–12,高通量v4:64(基于40倍平均覆盖度和90%以上的目标碱基覆盖度≥10倍) | 2 × 100 bp (最大推荐值) |
HiSeq 3000 System | 每次运行48个样本(高通量;基于40倍平均覆盖度和90%以上的目标碱基覆盖度≥10倍) | 2 × 100 bp (最大推荐值) |
HiSeq 4000 System | 每次运行96个样本(高通量;基于40倍平均覆盖度和90%以上的目标碱基覆盖度≥10倍) | 2 × 100 bp (最大推荐值) |
TruSeq Methyl Capture EPIC Library Prep Kit | Infinium MethylationEPIC Kit |
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This kit requires a PCR enzyme kit such as the Kapa Hifi Uracil+ (Cat No. KK2801), available separately.