Targeted methylation sequencing (Methyl-Seq) offers a balanced, cost-effective choice between whole-genome bisulfite sequencing and methylation arrays that can support both screening and biomarker discovery study objectives.1 TruSeq Methyl Capture EPIC Library Prep supports researchers seeking a deeper understanding of the role of methylation in gene regulation.
Comprehensive methylation-site content: Offers significantly higher coverage of emerging epigenetic regions of interest compared to other Methyl-Seq kits currently on the market (See Supporting Data section, Epigenetic Regions Covered).
Maximize discovery power, minimize cost: Efficiently query differentially methylated bases to conserve research dollars.
Convenient kit configuration: Includes bisulfite conversion, library prep, target enrichment, and purification reagents in a single kit for hassle-free ordering.
User-friendly data analysis: Analyze sequence data in BaseSpace Sequence Hub with methylation apps designed for biologists.
| 仪器 | 推荐的样本数 | 读长 |
|---|---|---|
| NextSeq 550 System | 每次运行8个样本（高通量；基于40倍平均覆盖度和90%以上的目标碱基覆盖度≥10倍） | 2 x 100 bp (最大推荐值) |
| NovaSeq 6000 System | 每次运行的样本（双流动槽）：S1：48，S2：48，S4：96（受可用标签组合的限制；基于＞5500万条read） | 2 × 100 bp (最大推荐值) |
| HiSeq 2500 System | 每次运行的样本：快速运行：8–12，高通量v4：64（基于40倍平均覆盖度和90%以上的目标碱基覆盖度≥10倍） | 2 × 100 bp (最大推荐值) |
| HiSeq 3000 System | 每次运行48个样本（高通量；基于40倍平均覆盖度和90%以上的目标碱基覆盖度≥10倍） | 2 × 100 bp (最大推荐值) |
| HiSeq 4000 System | 每次运行96个样本（高通量；基于40倍平均覆盖度和90%以上的目标碱基覆盖度≥10倍） | 2 × 100 bp (最大推荐值) |
| TruSeq Methyl Capture EPIC Library Prep Kit | Infinium MethylationEPIC Kit | |
|---|---|---|
| 变异种类 | 差异甲基化胞嘧啶 | 差异甲基化胞嘧啶 |
| 技术 | 测序 | 微阵列芯片 |
| 核酸类型 | DNA | DNA |
| 物种类别 | 人类 | 人类 |
| 系统兼容性 | NextSeq 500 , HiSeq 2500 , NextSeq 550 , HiSeq 3000 , HiSeq 4000 | iScan |
| 起始量 | 500 ng人类基因组DNA | 250 ng DNA |
The TruSeq Methyl Capture EPIC Library Prep Kit uniquely targets emerging epigenetic regions of interest such as open chromatin, ENCODE, and FANTOM regions. The percentage of genomic regions covered using the TruSeq Methyl Capture EPIC Library Prep Kit, the Competitor X Methyl-Seq Kit2 and the Competitor Y Methyl-Seq Kit3 are shown.
This kit requires a PCR enzyme kit such as the Kapa Hifi Uracil+ (Cat No. KK2801), available separately.
Robust methylation profiling microarray with unparalleled coverage of CpG islands, genes, and enhancers. Use for epigenome-wide association studies.
Targeted panel that measures expression levels of >20,000 human RefSeq genes.
Targeted panel investigating the exonic regions of 409 genes with known associations to cancer.
