SureCell WTA 3′ Library Prep Kit for the ddSEQ System

A robust, scalable, and user-friendly workflow for transcriptome profiling of hundreds to tens of thousands of single cells in a given experiment.Read More...
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SureCell™ Whole Transcriptome Analysis 3' Library Prep Kit (2 Cartridges Kit)

20014279

SureCell™ Whole Transcriptome Analysis 3' Library Prep Kit (6 Cartridges Kit)

20014280

产品特色

The Illumina Bio-Rad® SureCell™ Whole Transcriptome Analysis (WTA) 3’ Kit provides a validated process for single-cell RNA sequencing studies.

  • Sensitive and unbiased characterization of transcriptional signatures – Highly sensitive and reproducible gene detection in varied individual cells
  • Comprehensive single-cell RNA sequencing workflow – Fully supported workflow developed in collaboration by technology innovators
  • Powerful next-generation sequencing integrated with simple data analysis – Proven Illumina sequencing combined with streamlined, user-friendly analysis software

Single cells are individually partitioned into subnanoliter droplets on a disposable cartridge on the one-touch ddSEQ™ Single-Cell Isolator. The cartridge can accommodate multiple samples, and multiple cartridges can be processed in parallel to isolate thousands of cells in a matter of minutes.

Cell lysis and cell barcoding occur inside individual droplets, and single-cell-barcoded RNA-Seq libraries are subsequently prepared using Nextera technology.

Data Analysis

Conduct data analysis via BaseSpace Sequence Hub, the Illumina cloud-based genomics computing environment.

View sample data (requires a BaseSpace login)

Species Details

This kit has been validated for human and mouse samples. The BaseSpace Sequence Hub SureCell RNA Single-Cell App supports analysis of human, mouse, rat, zebrafish, C. elegans, and Drosophila samples.

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规格

计划推荐

仪器 推荐的样本数 读长
NextSeq 550 System 每次运行4至6个样本(基于每个细胞至少100,000条read) read 1: 68 bp, read 2: 75 bp
HiSeq 2500 System 快速运行:每条通道4–5个样本;高通量v4:每条通道4个样本(基于每个细胞至少100,000条read) read 1: 68 bp, read 2: 75 bp

产品比较

SureCell WTA 3′ Library Prep Kit for the ddSEQ System TruSeq RNA Library Prep Kit v2 TruSeq Stranded mRNA TruSeq RNA Exome

Method-Specific Workflow Example

 

Supporting Data and Figures

 

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