SureCell WTA 3′ Library Prep Kit for the ddSEQ System

A robust, scalable, and user-friendly workflow for transcriptome profiling of hundreds to tens of thousands of single cells in a given experiment.Read More...
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SureCell™ Whole Transcriptome Analysis 3' Library Prep Kit (2 Cartridges Kit)

20014279

SureCell™ Whole Transcriptome Analysis 3' Library Prep Kit (6 Cartridges Kit)

20014280

Product Highlights

The Illumina Bio-Rad® SureCell™ Whole Transcriptome Analysis (WTA) 3’ Kit provides a validated process for single-cell RNA sequencing studies.

  • Sensitive and unbiased characterization of transcriptional signatures – Highly sensitive and reproducible gene detection in varied individual cells
  • Comprehensive single-cell RNA sequencing workflow – Fully supported workflow developed in collaboration by technology innovators
  • Powerful next-generation sequencing integrated with simple data analysis – Proven Illumina sequencing combined with streamlined, user-friendly analysis software

Single cells are individually partitioned into subnanoliter droplets on a disposable cartridge on the one-touch ddSEQ™ Single-Cell Isolator. The cartridge can accommodate multiple samples, and multiple cartridges can be processed in parallel to isolate thousands of cells in a matter of minutes.

Cell lysis and cell barcoding occur inside individual droplets, and single-cell-barcoded RNA-Seq libraries are subsequently prepared using Nextera technology.

Data Analysis

Conduct data analysis via BaseSpace Sequence Hub, the Illumina cloud-based genomics computing environment.

View sample data (requires a BaseSpace login)

Species Details

This kit has been validated for human and mouse samples. The BaseSpace Sequence Hub SureCell RNA Single-Cell App supports analysis of human, mouse, rat, zebrafish, C. elegans, and Drosophila samples.

Frequently Purchased Together

规格

计划推荐

仪器 推荐的样本数 读长
NextSeq 550 System 每次运行4至6个样本(基于每个细胞至少100,000条read) read 1: 68 bp, read 2: 75 bp
HiSeq 2500 System 快速运行:每条通道4–5个样本;高通量v4:每条通道4个样本(基于每个细胞至少100,000条read) read 1: 68 bp, read 2: 75 bp

产品比较

SureCell WTA 3′ Library Prep Kit for the ddSEQ System TruSeq RNA Library Prep Kit v2 TruSeq链式mRNA文库制备试剂盒 TruSeq RNA Exome
技术 测序 测序 测序 测序
方法 mRNA测序 mRNA测序 mRNA测序 mRNA测序 , 外显子组测序
核酸类型 RNA RNA RNA RNA
物种类别 人类, 小鼠 哺乳动物, 大鼠, 人类, 牛, 小鼠 大鼠, 人类, 小鼠, 牛, 哺乳动物, 其他 人类
系统兼容性 NextSeq 550 , NextSeq 500 , HiSeq 4000 , HiSeq 1500, HiSeq 3000 , HiSeq 1000, HiSeq 2000, HiSeq 2500 HiSeq 1000, HiSeq 4000 , NextSeq 550 , HiSeq 2000, HiSeq 1500, MiSeq , MiSeq, NextSeq 500 , Genome Analyzer IIx, HiSeq 3000 , HiSeq 2500 HiSeq 3000 , Genome Analyzer IIx, NextSeq 500 , HiSeq 4000 , MiSeq , MiSeq, HiSeq 1500, HiSeq 2500 , HiSeq 2000, NextSeq 550 , HiSeq 1000 HiSeq 4000 , NextSeq 500 , HiSeq 3000 , HiSeq 2500 , NextSeq 550

Method-Specific Workflow Example

 

Supporting Data and Figures

 

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