SureCell WTA 3′ Library Prep Kit for the ddSEQ System

A robust, scalable, and user-friendly workflow for transcriptome profiling of hundreds to tens of thousands of single cells in a given experiment.Read More...

SureCell™ Whole Transcriptome Analysis 3' Library Prep Kit (2 Cartridges Kit)


SureCell™ Whole Transcriptome Analysis 3' Library Prep Kit (6 Cartridges Kit)


Product Highlights

The Illumina Bio-Rad® SureCell™ Whole Transcriptome Analysis (WTA) 3’ Kit provides a validated process for single-cell RNA sequencing studies.

  • Sensitive and unbiased characterization of transcriptional signatures – Highly sensitive and reproducible gene detection in varied individual cells
  • Comprehensive single-cell RNA sequencing workflow – Fully supported workflow developed in collaboration by technology innovators
  • Powerful next-generation sequencing integrated with simple data analysis – Proven Illumina sequencing combined with streamlined, user-friendly analysis software

Single cells are individually partitioned into subnanoliter droplets on a disposable cartridge on the one-touch ddSEQ™ Single-Cell Isolator. The cartridge can accommodate multiple samples, and multiple cartridges can be processed in parallel to isolate thousands of cells in a matter of minutes.

Cell lysis and cell barcoding occur inside individual droplets, and single-cell-barcoded RNA-Seq libraries are subsequently prepared using Nextera technology.

Data Analysis

Conduct data analysis via BaseSpace Sequence Hub, the Illumina cloud-based genomics computing environment.

View sample data (requires a BaseSpace login)

Species Details

This kit has been validated for human and mouse samples. The BaseSpace Sequence Hub SureCell RNA Single-Cell App supports analysis of human, mouse, rat, zebrafish, C. elegans, and Drosophila samples.

Frequently Purchased Together



仪器 推荐的样本数 读长
NextSeq 550 System 每次运行4至6个样本(基于每个细胞至少100,000条read) read 1: 68 bp, read 2: 75 bp
HiSeq 2500 System 快速运行:每条通道4–5个样本;高通量v4:每条通道4个样本(基于每个细胞至少100,000条read) read 1: 68 bp, read 2: 75 bp


SureCell WTA 3′ Library Prep Kit for the ddSEQ System TruSeq RNA Library Prep Kit v2 TruSeq链式mRNA文库制备试剂盒 TruSeq RNA Exome
技术 测序 测序 测序 测序
方法 mRNA测序 mRNA测序 mRNA测序 mRNA测序 , 外显子组测序
物种类别 人类, 小鼠 哺乳动物, 大鼠, 人类, 牛, 小鼠 大鼠, 人类, 小鼠, 牛, 哺乳动物, 其他 人类
系统兼容性 NextSeq 550 , NextSeq 500 , HiSeq 4000 , HiSeq 1500, HiSeq 3000 , HiSeq 1000, HiSeq 2000, HiSeq 2500 HiSeq 1000, HiSeq 4000 , NextSeq 550 , HiSeq 2000, HiSeq 1500, MiSeq , MiSeq, NextSeq 500 , Genome Analyzer IIx, HiSeq 3000 , HiSeq 2500 HiSeq 3000 , Genome Analyzer IIx, NextSeq 500 , HiSeq 4000 , MiSeq , MiSeq, HiSeq 1500, HiSeq 2500 , HiSeq 2000, NextSeq 550 , HiSeq 1000 HiSeq 4000 , NextSeq 500 , HiSeq 3000 , HiSeq 2500 , NextSeq 550

Method-Specific Workflow Example


Supporting Data and Figures


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