SureCell WTA 3′ Library Prep Kit for the ddSEQ System

A robust, scalable, and user-friendly workflow for transcriptome profiling of hundreds to tens of thousands of single cells in a given experiment. Read More...
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SureCell™ Whole Transcriptome Analysis 3' Library Prep Kit (2 Cartridges Kit)

20014279

SureCell™ Whole Transcriptome Analysis 3' Library Prep Kit (6 Cartridges Kit)

20014280

产品特色

The Illumina Bio-Rad® SureCell™ Whole Transcriptome Analysis (WTA) 3’ Kit provides a validated process for single-cell RNA sequencing studies.

  • Sensitive and unbiased characterization of transcriptional signatures – Highly sensitive and reproducible gene detection in varied individual cells
  • Comprehensive single-cell RNA sequencing workflow – Fully supported workflow developed in collaboration by technology innovators
  • Powerful next-generation sequencing integrated with simple data analysis – Proven Illumina sequencing combined with streamlined, user-friendly analysis software

Single cells are individually partitioned into subnanoliter droplets on a disposable cartridge on the one-touch ddSEQ™ Single-Cell Isolator. The cartridge can accommodate multiple samples, and multiple cartridges can be processed in parallel to isolate thousands of cells in a matter of minutes.

Cell lysis and cell barcoding occur inside individual droplets, and single-cell-barcoded RNA-Seq libraries are subsequently prepared using Nextera technology.

Data Analysis

Conduct data analysis via BaseSpace Sequence Hub, the Illumina cloud-based genomics computing environment.

View sample data (requires a BaseSpace login)

Species Details

This kit has been validated for human and mouse samples. The BaseSpace Sequence Hub SureCell RNA Single-Cell App supports analysis of human, mouse, rat, zebrafish, C. elegans, and Drosophila samples.

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规格

计划推荐

仪器 推荐的样本数 读长
NextSeq 550 System 4 to 6 samples per run (based on a minimum of 100,000 reads per cell) read 1: 68 bp, read 2: 75 bp
HiSeq 2500 System Rapid run: 4-5 samples per lane; high output v4: 4 samples per lane (based on a minimum of 100,000 reads per cell) read 1: 68 bp, read 2: 75 bp

产品比较

SureCell WTA 3′ Library Prep Kit for the ddSEQ System TruSeq RNA Library Prep Kit v2 Illumina Stranded mRNA Prep Illumina RNA Prep with Enrichment
Assay Time 11-12 hours ~10.5 hours 6.5小时 <9小时
Content Specifications Captures the coding transcriptome (3' enriched), with strand-specific information Captures the coding transcriptome (without strand information) 捕获编码转录组(带链信息) 与Illumina Exome Panel一起使用时可捕获编码转录组
Description Enables single-cell RNA-Seq studies using a droplet generator that isolates ~1200 cells per cartridge, and a simple library prep process. A simple, cost-effective research solution for analysis of the coding transcriptome. 简单、经济的编码转录组分析解决方案,可提供精确的链信息 一种可重现且经济的解决方案,可使用多种类型的样本和起始材料来检测和发现目标转录本,包括福尔马林固定石蜡包埋(FFPE)组织和其他低质量样本
Hands-On Time 4-5 hours ~4.5 hours <3小时 <2小时
Input Quantity ~45,000 cells per cartridge (required input) 0.1 - 1 ug total RNA or 10 - 400 ng previously isolated mRNA (from species with polyA tails) 25-1000 ng标准质量的总RNA 10 ng新鲜/冷冻样本来源的总RNA,或20 ng FFPE样本来源的总RNA
Mechanism of Action Oligo dT capture, single cell Oligo-dT beads capture polyA tails PolyA捕获,连接接头和标签 连接磁珠的转座酶
Method mRNA Sequencing mRNA Sequencing mRNA Sequencing mRNA Sequencing, 靶向RNA测序, 靶向富集
Multiplexing 1-24 Up to 24-plex per lane 多达384个唯一双标签序列(UDI) 多达384个唯一双标签序列(UDI)
Specialized Sample Types Single Cells, 不兼容FFPE 不兼容FFPE Low-Input Samples, Not FFPE-Compatible Blood, FFPE Tissue, Low-Input Samples
Species Category 人类, 小鼠 Bovine, Mammalian, 人类, 大鼠, 小鼠 Bovine, Mammalian, 人类, 大鼠, 小鼠 Virus, 人类
Strand Specificity Stranded Non-Stranded Stranded Non-Stranded

Method-Specific Workflow Example

 

Supporting Data and Figures

 

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