TruSight Cardio Sequencing Kit for NextSeq v2 (FC-141-1011) has been discontinued; the recommended replacement is TruSight Cardio Sequencing Kit for NextSeq v2.5 (20035190). The library prep components of the kit have not changed. Please contact your local sales representative for additional information on this change.
The TruSight Cardio Kit uses next-generation sequencing (NGS) to provide comprehensive coverage of 174 genes with known associations to 17 ICCs, including cardiomyopathies, arrhythmias, aortopathies, and more. Genes were expertly selected with researchers at the National Heart Centre Singapore and Imperial College of London. Content includes genes known to be associated with hereditary heart disease and emerging genes found in the literature.1
Download this reference guide to understand which kit quantities you should purchase to support your research.
Download Reference Guide
| 仪器 | 推荐的样本数 | 读长 |
|---|---|---|
| MiSeq System | 12 samples per run with v2 reagents (based on 300x mean coverage of targeted content) | 2 × 150 bp (max recommended) |
| NextSeq 550 System | 48 samples per run (mid output; based on 300x mean coverage of targeted content) | 2 × 150 bp (max recommended) |
| TruSight Cardio Sequencing Panel | TruSight One Sequencing Panels | |
|---|---|---|
| 内容说明 | 575 Kb基因组内容（174个基因） | TruSight One：约12 Mb基因组内容（约4800个基因）。 TruSight One Expanded：约16.5 Mb基因组内容（约6700个基因）。 |
| 多重分析 | 多达96重 | 多达96重 |
| 方法 | 靶向富集, 靶向DNA测序 | 靶向富集, 靶向DNA测序 |
| 物种类别 | 人类 | 人类 |
| 特殊的样本类型 | Not FFPE-Compatible | Not FFPE-Compatible |
| 说明 | 此款靶向测序研究panel有助于鉴定与17种遗传性心脏病相关的遗传变异。 | 固定基因集的panel，专注于具有致病变异的外显子区域。 |
| 起始量 | 50 ng DNA | 50 ng DNA |
TruSight Cardio Sequencing Kit Gene List
product_file | EXCEL < 1 MB
TruSight Fixed Panel Combo Order Quantities
Product Information Sheet | PDF < 1 MB
TruSight Cardio Sequencing Panel
Data Sheet | PDF < 1 MB
TruSight Cardio Sequencing Kit Support Documentation
TruSight Cardio Sequencing Kit Checklist Documentation
TruSight Cardio Sequencing Kit Reference Guide Documentation
自定义协议选择器
Generates customized, end-to-end instructions
The TruSight Cardiomyopathy Sequencing Panel has been discontinued. The TruSight Cardio Sequencing Kit is the recommended replacement.
* Data on file. Illumina, Inc. 2015.