TruSight Cardio Sequencing Kit

此试剂盒为研究人员提供了鉴定遗传性心脏病(ICC)致病变异的全面、经济的解决方案。Read More...
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Bundled Solutions

TruSight Cardio Sequencing Kit for NextSeq v2.5 (48 indexes, 48 samples, 4 enrichments)

20035190

TruSight Cardio Sequencing Kit for MiSeq and MiSeqDx (12 indexes, 12 samples, 1 enrichment)

FC-141-1010


Enrichment Panels

TruSight Cardio – Enrichment Oligos only (8 Enrichment Reactions)

20029229

配件产品

Illumina® DNA Prep with Enrichment, (S) Tagmentation (96 Samples)

20025524

Illumina® DNA Prep with Enrichment, (S) Tagmentation (16 Samples)

20025523

Illumina® DNA Prep, (S) Tagmentation (96 Samples)

20025520

Illumina® DNA Prep, (S) Tagmentation (16 Samples)

20025519

IDT® for Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)

20027213

产品特色

  • Expertly defined genes selected in collaboration with the Imperial College of London
  • 99% of the targeted regions covered at a depth of at least 20×*
  • Cost to sequence is ~ $1 US per gene; fully supported on Illumina sequencing and informatics platforms

The TruSight Cardio Kit uses next-generation sequencing (NGS) to provide comprehensive coverage of 174 genes with known associations to 17 ICCs, including cardiomyopathies, arrhythmias, aortopathies, and more. Genes were expertly selected with researchers at the National Heart Centre Singapore and Imperial College of London. Content includes genes known to be associated with hereditary heart disease and emerging genes found in the literature.1

Frequently Purchased Together

Plan on Using the TruSight Cardio Sequencing Kit with MiSeq v3?

Download this reference guide to understand which kit quantities you should purchase to support your research.

Download Reference Guide
Reference guide: TruSight Cardio Sequencing Kit

规格

计划推荐

仪器 推荐的样本数 读长
MiSeq System 12 samples per run with v2 reagents (based on 300x mean coverage of targeted content) 2 × 150 bp (max recommended)
NextSeq 550 System 48 samples per run (mid output; based on 300x mean coverage of targeted content) 2 × 150 bp (max recommended)

产品比较

TruSight Cardio Sequencing Kit TruSight One Sequencing Panels TruSight Inherited Disease
Content Specifications 575 Kb基因组内容(174个基因) TruSight One:约12 Mb基因组内容(约4800个基因)。
TruSight One Expanded:约16.5 Mb基因组内容(约6700个基因)。
2.25 Mb genomic content (552 genes)
Description 此款靶向测序研究panel有助于鉴定与17种遗传性心脏病相关的遗传变异。 固定基因集的panel,专注于具有致病变异的外显子区域。 Fixed research panel focused on severe pediatric onset disorders.
Input Quantity 50 ng DNA 50 ng DNA 50 ng DNA
Method 靶向DNA测序 , 靶向富集 靶向DNA测序 , 靶向富集 靶向DNA测序 , 靶向富集
Multiplexing 多达96重 多达96重 Up to 96-plex
Specialized Sample Types Not FFPE-Compatible Not FFPE-Compatible Not FFPE-Compatible

Method-Specific Workflow Example

 

The TruSight Cardiomyopathy Sequencing Panel has been discontinued. The TruSight Cardio Sequencing Kit is the recommended replacement.

Related Products to Consider

References
  1. Pua CJ, Bhalshankar J, Miao K, et al. Development of a comprehensive sequencing assay for inherited cardiac condition genes. J Cardiovasc Trans Res. 2016;9:3.

* Data on file. Illumina, Inc. 2015.