微生物群落会影响宿主的免疫应答
宿主体内的微生物会影响癌症进展和治疗有效性。饮食和药物会破坏微生物组的多样性，微生物组中的关键物种会对宿主的免疫力产生局部或全局的影响。1,2
未来的治疗方法有可能会结合现有的癌症疗法与其他能促进有益微生物生长或消除有害微生物的方法。随着基于NGS的研究继续探索宿主与微生物组的相互关系，Illumina正在努力开发可加强该领域并为其带来前景的基因组技术。
癌症免疫疗法与微生物组的作用
现代癌症免疫疗法的有效性受肠道微生物组组成的影响。
Microbes Can Exert Varied Effects on Cancer Progression
Microbes that influence cytokine release and inflammation can exert either a positive or negative effect on tumor growth, or the ability of the immune system to suppress tumor growth.
NGS allows profiling of microbial communities in different contexts, which is critical for identifying species or conditions that may be targeted for developing new therapeutic approaches to cancer.
NGS Methods to Study the Role of the Microbiome in Cancer
The ability of NGS to analyze complex microbial communities may help to uncover new mechanisms of host–microbe interactions that promote cancer or promote drug efficacy.
The throughput and cost savings of NGS have fueled metagenomics studies capable of surveying the genomes of entire microbial communities.
NGS methods that can provide critical genetic insight into the microbiome:
Shotgun Metagenomic Sequencing
A DNA sequencing method that enables comprehensive sampling of all genes in all organisms in complex microbiome samples.
16S rRNA Sequencing
A 16S ribosomal RNA gene sequencing method used to identify and compare bacteria present within a given sample.
Microbial Metatranscriptomics
Analysis of all RNAs encoded by a group of microorganisms within a complex sample.
How Microbiome Multiomics Can Bolster Human Health
Sequencing technologies are enabling a deeper analysis of the gut's microbiome. Researchers can now explore what our microbial inhabitants are doing and how they contribute to, or protect from, diseases such as cancer and autoimmune disorders.
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癌症微生物组分析工作流程
NGS具有极高的通量和灵敏度，可鉴定单个样本中的数千种微生物。
点击下方的查看工作流程各步骤所使用的产品。
TruSeq Stranded Total RNA Library Prep Kit
简便、经济、可扩展的总RNA分析解决方案。
Nextera XT Library Prep Kit制备细菌、病毒和其他微生物的可直接用于测序的文库，用于分析转录组和宏转录组信息。
TruSeq DNA PCR-Free Library Preparation Kit
为全基因组测序应用提供简单全面的文库制备方案。研究人员可对多种生物进行测序，从细菌等小型基因组到人类全基因组。
16S Metagenomics
利用Illumina审核的GreenGenes分类数据库对16S rRNA靶向扩增子read进行分类学分类。
Kraken宏基因组学使用精准k-mer比对和新颖的分类算法，将分类学标签高度灵敏地快速分配给短DNA序列。
Gene-Level Metagenomics for Microbiome Research
Dr. Samuel Minot from the Fred Hutchinson Cancer Research Center discusses a novel gene-level bioinformatics approach for identifying microbial species and strains associated with human diseases such as cancer, from metagenomic sequencing datasets.
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Related Applications
Analyze Gene Expression and Chromatin Accessibility
Unify single-cell gene expression and ATAC-Seq to help reveal cellular mechanisms driving gene regulation.
Read Tech NoteMeasure Protein Expression Along with RNA-Seq
Learn how to incorporate protein detection into bulk RNA-Seq and develop a workflow for BEN-Seq, providing a holistic approach to cell analysis.
Read App Note更多资源
HPV and Skin Cancer
Illumina sequencers offer deep coverage to identify novel HPV types correlated with non-melanoma skin cancers.
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注册参考文献
- Zama D, Biagi E, Masetti R, et al.Gut microbiota and hematopoietic stem cell transplantation: where do we stand? Bone Marrow Transplant.2017;52 (1):7-14.
- Schwabe R, Jobin C. The microbiome and cancer.Nat Rev Cancer.2013;13 (11):800-812.
- Franzosa EA, Hsu T, Sirota-Madi A, et al.Sequencing and beyond: integrating molecular 'omics' for microbial community profiling.Nat Rev Microbiol.2015;13(6):360-372.