Sample Multiplexing is a useful technique when targeting specific genomic regions or working with smaller genomes. Pooling samples into a single lane of a flow cell exponentially increases the number of samples analyzed in a single run without drastically increasing cost or time.

To prepare samples for multiplexing, a unique identifier tag, or index, is added to each library. Using the Genome Analyzer_IIx system an automated three-read sequencing strategy accurately identifies each uniquely-tagged sample for individual downstream analysis.

Multiplex Sequencing Highlights

 

• Fast, High-Throughput Strategy: Automated sequencing of up to 12 samples per lane or 96 samples per flow cell

 

 

• Cost-Effective Method: Multisample pooling improves productivity by reducing time and reagent use

 

 

• High-Quality Data: Accurate maintenance of read length of unknown sequences

 

 

• Simplified Analysis: Automatic sample identification with index using the Pipeline Analysis software