Illumina BeadArray technology
BeadArray technology: A fundamentally different approach to high-density microarrays

Learn more about the iScan and HiScanSQ systems

一种新型微阵列方法

Illumina微珠列阵阵列技术是以下列两种基质微孔中自组装的3微米硅片以为基础：集束光纤或平面硅片。在任一种基质随机组装时，微珠的间距一致且大约保持在5.7微米。每一微珠上都覆有某一特定寡聚核苷酸的成百上千条拷贝，在Illumina检测中，这些拷贝将作为其中的捕获序列。微珠阵列技术可用于Illumina的iScan 系统 ，从而广泛用于对DNA和RNA的分析。


The original Infinium II Assay was great, but Infinium HD is remarkable. Most samples have an average call rate of 99.9% off the shelf, which makes downstream curation of data a whole lot easier. For those who aren’t curating their data, there's much less downstream work because there is less likelihood of false positives and negatives due to the higher sample call rates. In addition to data quality, the ability to run four samples per chip clearly gets to the answers a lot faster.

多样品阵列模式
微珠阵列技术可与任一多样品阵列模式相结合，从而用于DNA或RNA的分析中。使用96样品阵列矩阵和多样本BeadChip芯片模式，组装前每种基质表面都蚀刻相同凹槽，凹槽深度约为3毫米。随后，在范德华力和与微孔壁间流体静力学相互作用下，微珠可在上述微孔内进行随机组装并固定。

这种96样品的阵列短阵模式已用于Illumina的GoldenGate基因分型、DASL基因表达以及聚焦阵列程序中。

Illumina的Infinium基因分型、DASL基因表达以及聚焦阵列程序均采用了BeadChip芯片模式。

Illumina multi-sample array formats