Illumina HiSeq 2000

HiSeq 2000


应用: DNA 测序, 基因调控分析, 测序法转录组分析, SNP发现与结构变异分析, 细胞遗传学分析, DNA-蛋白质相互作用分析(ChIP-Seq), 测序法甲基化分析, 小RNA发现及分析


HiSeq 2000测序系统带来了前所未有的产量和突破性的用户体验。凭借Illumina久经考验且广泛采用的可逆终止法边合成边测序试剂,再加上创新的制造工艺,HiSeq 2000实现了行业最高的测序产量和最快的数据产生速率。人机交互设计特征以及最轻松的测序流程,为简便性及用户体验设立了全新的标准。
更 多...


索取报价      QUESTIONS?

系统描述

凭借创新的设计特征,HiSeq 2000成为最易于使用的新一代测序系统。

  • 流动槽很容易上样到真空控制的上样站中。
  • 只需要2分钟的手工操作时间,即可将预先配置足够200个循环使用的、即插即用型试剂安置入仪器冷冻室的架子上。
  • 简单的触摸屏用户界面,提供屏幕显示的每一步操作指示和嵌入的多媒体协助,简化了运行设置。
  • 实时的进展显示可提供一目了然的状态,而远程监控让单个用户能够通过任何一个浏览器或者可连接互联网的电话来检查多个系统的进展。
  • HiSeq 2000能够以单个或两个流动槽模式进行操作,赋予了无可比拟的实验灵活性和仪器可扩展性。
  • 可独立操控的流动槽让需要不同读长的多个应用能同时运行。
  • 内含Illumina高效且可扩展的数据分析方案,能将数十亿个碱基的原始测序数据转化成可发表的,在生物学上有意义的结果。

 

优势

前所未有的高产量

每次运行多达200 Gb,2×100 bp的读长,每天能达到25 Gb,每次运行达200亿个配对末端读取。

突破性的用户体验

预先配置、即插即用的试剂,简单的流动槽上样,便于指导每一步运行设置的触摸屏式用户界面,以及整合的配对末端流体学设计。

无可比拟的经济操作

单次运行即可以~30倍的覆盖度同时对两个人类基因组样品进行测序,每个基因组的费用不到1万美元;或同时绘制200个基因表达谱,每个样品不到200美元。

 

以前所未有的规模测序

HiSeq 2000是市场上第一台能够让研究人员在单次运行中获得两个人类基因组的测序仪,每个基因组的覆盖度约为30倍,费用低于1万美元*。

 

HiSeq 2000让个体实验室也能承担最大最复杂的测序研究,而费用最低。处理更多样品及解码更大更复杂基因组的能力意味着几乎所有测序项目都触手可及。

 

*试剂费用为目录价。

会议消息

European Society of Human Genetics (ESHG)
June 12-15, 2010
Gothenburg, Sweden

流程 & 规格

HiSeq 2000 Preliminary Performance SpecificationsHiSeq 2000 Workflow

配件&附件

cBot

IlluminaCompute

Filter by Application
Filter by Assays

HiSeq 2000 Kits

cBot Single Read Cluster Generation Kit

cBot Single Read Cluster Generation Kit

The cBot Single Read Cluster Generation Kit provides reagents that bind DNA library samples to complementary adapter oligonucleotides grafted on the flow cell surface. The pre-mixed, 96-well plate format reagents in this kit provide a streamlined workflow with no reagent preparation and a total of 10 minutes hands-on time.

Additional Details

GD-300-1001
ADD TO CART
cBot Paired-End Cluster Generation Kit

cBot Paired-End Cluster Generation Kit

The cBot Paired-End Cluster Generation Kit provides reagents that bind samples to complementary adapter oligos on the flow cell. These enable copying of the DNA strand after the first sequencing run; the copied strand is used to sequence the other fragment end. The cBot amplifies attached DNA fragments to clonal clusters of ~1000 copies each.

Additional Details

PE-300-1001
ADD TO CART
HiSeq 2000 Paired-End Cluster Generation Kit

HiSeq 2000 Paired-End Cluster Generation Kit

The HiSeq Paired-End Cluster Generation Kit provides reagents that bind samples to complementary adapter oligos on the flow cell. These enable copying of the DNA strand after the first sequencing run; the copied strand is used to sequence the other fragment end. cBot amplifies attached DNA fragments to clonal clusters of ~1000 copies each.

Additional Details

PE-401-1001
ADD TO CART
HiSeq 2000 Single-Read Cluster Generation Kit

HiSeq 2000 Single-Read Cluster Generation Kit

The HiSeq Single-Read Cluster Generation Kit provides reagents that bind samples to complementary adapter oligos on the flow cell.

Additional Details

GD-401-1001
ADD TO CART
Small RNA Sample Prep Kit

Small RNA Sample Prep Kit

With Small RNA Discovery and Analysis, Illumina scientists have designed a sample preparation protocol that enables sequencing of of known and novel microRNAs as well as other non-coding RNAs, such as piwi-interacting RNAs and siRNAs. Sequencing-based Small RNA Sample Prep Kits offer researchers the flexibility to select the length of the small RNA they wish to investigate, enabling size-focused or broad size range investigation of different classes of small RNA on one universal platform.

Additional Details

FC-102-1009
ADD TO CART
Paired-End DNA Sample Prep Kit V1

Paired-End DNA Sample Prep Kit V1

The Paired-End DNA Sample Prep Kit helps build DNA libraries for paired-end sequencing with insert sizes from 200-500 bp. Preparation includes introduction of two unique sequencing primer binding sites and adapter sequences to the anti-parallel strands of the polished whole-genome DNA fragments followed by gel-based size selection and purification.

Additional Details

PE-102-1001
ADD TO CART
mRNA-Seq 8-Sample Prep Kit

mRNA-Seq 8-Sample Prep Kit

The mRNA-Seq 8-Sample Prep Kit is used to build DNA libraries for single-read and paired-end sequencing on the Genome Analyzer. Sample preparation is straightforward, using standard molecular biology techniques and requiring minimal hands-on time. mRNA template libraries are sequenced just as any DNA sample using Illumina Sequencing.

Additional Details

RS-100-0801
ADD TO CART
Multiplexing Sample Preparation Oligonucleotide Kit

Multiplexing Sample Preparation Oligonucleotide Kit

The Multiplexing Sample Preparation Oligonucleotide Kit contains 12 unique oligonucleotides to “tag” libraries for pooling in one flow cell lane. Up to 96 samples can be sequenced on a single flow cell using the Genome Analyzer. The multiplex sequencing process is fully automated using the Cluster Station, Genome Analyzer, and Paired-End Module.

Additional Details

PE-400-1001
ADD TO CART
Mate Pair Library Prep Kit

Mate Pair Library Prep Kit

The Mate Pair Library Prep Kit includes reagents needed to generate libraries with inserts from 2-5 kb in size. Prepared libraries consist of short fragments made up of two segments that were originally separated by several kilobases in the genome. Mate Pairs are sequenced using a two-adapter strategy identical to the paired-end sequencing method.

Additional Details

PE-112-1002
ADD TO CART
Genomic DNA Sample Prep Kits

Genomic DNA Sample Prep Kits

Go from purified genomic DNA to a DNA library. Genomic DNA Sample Prep Kits include reagents to build DNA libraries for single-read sequencing. Random size–selected fragments of DNA are created, polished, and prepared for the addition of unique adapters to fragment ends. Following a short PCR enrichment, the library is ready to load.

Additional Details

FC-102-1001
ADD TO CART
Tag Profiling Sample Prep Kits

Tag Profiling Sample Prep Kits

Tag Profiling Sample Prep Kits are used to build constructs comprised of a unique, positionally known 20- or 21-base pair cDNA tag with defined adapters attached to both ends. Access to any mRNA is through two restriction enzyme tag construction options. Kits are available complete or containing only the required oligonucleotides.

Additional Details

FC-102-1005
GET A QUOTE
ChIP-Seq DNA Sample Prep Kit

ChIP-Seq DNA Sample Prep Kit

Chromatin immunoprecipitation (ChIP) selectively finds DNA sequences bound by a particular protein. The ChIP-Seq DNA Sample Prep Kit helps build DNA libraries. The ChIP process enriches specific crosslinked DNA protein complexes using an antibody and unique oligonucleotide adapters added to small stretches of DNA bound to the protein of interest.

Additional Details

IP-102-1001
ADD TO CART
Multiplexing Sequencing Primers and PhiX Control Kit V2

Multiplexing Sequencing Primers and PhiX Control Kit V2

The Multiplexing Sequencing Primers and PhiX Control Kit V2 is used to sequence samples tagged by using the Multiplexing Sample Preparation Oligonucleotide Kit and then amplified in the cBot or Cluster Station. Sequencing is fully automated on an Illumina sequencing system.

Additional Details

PE-400-2002
ADD TO CART
TruSeq SBS V5 HS kits

TruSeq SBS V5 HS kits

TruSeq SBS V5 HS kits contain ready-to-load reagents for accurately determining the DNA sequence of each cluster on a flow cell using sequencing by synthesis technology on the HiSeq 2000 sequencing system.

Additional Details

FC-401-1001
ADD TO CART

软件

Illumina 引以为傲地提供了 系统和分析软件 旨在让您的数据质量和通量水平最大化。每台仪器都包括系统控制软件,以确保最佳性能。数据可通过Illumina用户友好的 GenomeStudio 软件t第三方软件包来分析。

支持

Illumina的客户解决方案团队致力于为您提供所需的资源,以支持您的研究。即使在您第一次购买仪器之前,也能得到精通Illumina技术、科学应用、软件及硬件系统的科学家的支持。现场服务工程师能熟练地安装仪器,并使其符合要求,以保证您有一个功能完全的系统。现场应用科学家提供密集的培训,让实验室能快速精通掌握如何以空前的通量水平产生行业领先的数据质量。只需一个电话或邮件,技术支持科学家就能协助您设计目、剖剖析问题并阐释结果。

我们一直在努力超越您的预期,以期您可在实验室中将Illumina技术的影响力发扬光大。如需了解更多信息,请访问支持部分。


FAQs