Illumina
Illumina cBot Cluster Generation System

cBot Cluster 生成系统


cBot是Illumina测序中新一代的工作流程改进。cBot这个创新的自动化系统，能从单分子DNA模板中构建克隆集群，为 Genome Analyzer IIx的边合成边测序做准备。 更多内容...


索取报价 WATCH THE VIDEO

System Description

The cluster generation process on cBot has been significantly streamlined. Innovative features such as pre-packaged reagents, a single flow cell manifold, and integrated touch screen controls make the best next generation sequencing workflow even better.

cBot isothermally amplifies cDNA fragments that have been captured by complementary adapter oligonucleotides covalently bound to the surface of Illumina flow cells. Flow cells facilitate access of bound DNA to enzymes while ensuring high stability of surface-bound template and low non-specific binding of fluorescently-labeled nucleotides. Attached DNA fragments are extended and bridge amplified to create hundreds of millions of clusters, each of which contains ~1,000 identical copies of a single template molecule.


应用: DNA测序 , 基因调控分析s , 基于测序的转录组分析 , SNP发现和结构变异分析 , 细胞遗传分析 , DNA-蛋白相互作用分析（ChIP-Seq） , 测序法甲基化分析 , 小RNA发现和分析

Show more applications...

优势

高效

4小时完成自动化Cluster生成，手工操作时间不到10分钟

易用

以96孔板形式预包装的即用型试剂，简单的触摸屏式仪器操控，基于网络浏览器的远程监控

最高质量的结果

智能传感器监控性能，改进的试剂及步骤，能获得更高密度的Clusters及更高的测序精确性

工作流程 & 参数

根据应用筛选
根据分析筛选

cBot Cluster 生成系统试剂盒

TruSeq SBS Kit v5–GA

TruSeq SBS Kit v5–GA

The Genome Analyzer IIx system requires the TruSeq SBS Kit v5–GA . With the TruSeq reagents, Genome Analyzer IIx users can extend their read-lengths to 2 x 150 and achieve up to 95 Gb of mappable data.

Additional Details

FC-104-5001
ADD TO CART
Paired-End DNA Sample Prep Kit

Paired-End DNA Sample Prep Kit

The Paired-End DNA Sample Prep Kit helps build DNA libraries for paired-end sequencing with insert sizes from 200-500 bp. Preparation includes introduction of two unique sequencing primer binding sites and adapter sequences to the anti-parallel strands of the polished whole-genome DNA fragments followed by gel-based size selection and purification.

Additional Details

PE-102-1001
ADD TO CART
Small RNA Cluster Generation Kits

Small RNA Cluster Generation Kits

For Digital Gene Expression (DGE): Small RNA Discovery and Analysis, the Small RNA Cluster Generation Kit is used to bind total RNA samples to complementary adapter oligos on a flow cell surface. The Cluster Station isothermally amplifies attached cDNA and resulting high-density template cluster array is sequenced by the Illumina Genome Analyzer.

Additional Details

FC-103-4001
ADD TO CART
Paired-End Cluster Generation Kit v4

Paired-End Cluster Generation Kit v4

The Paired-End Cluster Generation Kit v4 provides reagents that bind samples to complementary adapter oligos on the flow cell. These enable copying of the DNA strand after the first sequencing run; the copied strand is used to sequence the other fragment end. The Cluster Station amplifies attached DNA fragments to clonal clusters of ~1000 copies each.

Additional Details

PE-203-4001
ADD TO CART
Small RNA Sample Prep Kit

Small RNA Sample Prep Kit

With Small RNA Discovery and Analysis, Illumina scientists have designed a sample preparation protocol that enables sequencing of of known and novel microRNAs as well as other non-coding RNAs, such as piwi-interacting RNAs and siRNAs. Sequencing-based Small RNA Sample Prep Kits offer researchers the flexibility to select the length of the small RNA they wish to investigate, enabling size-focused or broad size range investigation of different classes of small RNA on one universal platform.

Additional Details

FC-102-1009
ADD TO CART
Standard Cluster Generation Kit v4

Standard Cluster Generation Kit v4

The Standard Cluster Generation Kit v4 provides ready-to-load reagents that bind DNA library samples to complementary adapter oligos grafted on the flow cell surface. The master-mixed reagents in this kit provide a streamlined workflow with a total of 10 minutes hands-on time for reagent preparation and setup.

Additional Details

GD-103-4001
ADD TO CART
Multiplexing Sequencing Primers and PhiX Control Kit V2

Multiplexing Sequencing Primers and PhiX Control Kit V2

The Multiplexing Sequencing Primers and PhiX Control Kit V2 is used to sequence samples tagged by using the Multiplexing Sample Preparation Oligonucleotide Kit and then amplified in the cBot or Cluster Station. Sequencing is fully automated on an Illumina sequencing system.

Additional Details

PE-400-2002
ADD TO CART
Multiplexing Sample Preparation Oligonucleotide Kit

Multiplexing Sample Preparation Oligonucleotide Kit

The Multiplexing Sample Preparation Oligonucleotide Kit contains 12 unique oligonucleotides to “tag” libraries for pooling in one flow cell lane. Up to 96 samples can be sequenced on a single flow cell using the Genome Analyzer. The multiplex sequencing process is fully automated using the Cluster Station, Genome Analyzer, and Paired-End Module.

Additional Details

PE-400-1001
ADD TO CART
Mate Pair Library Prep Kit v2

Mate Pair Library Prep Kit v2

The Mate Pair Library Prep Kit includes reagents needed to generate libraries with inserts from 2-5 kb in size. Prepared libraries consist of short fragments made up of two segments that were originally separated by several kilobases in the genome. Mate Pairs are sequenced using a two-adapter strategy identical to the paired-end sequencing method.

Additional Details

PE-112-2002
ADD TO CART
Illumina Sequencing Kits v4

Illumina Sequencing Kits v4

The Illumina Sequencing Kit v4 contains ready-to-load reagents for accurately determining the DNA sequence of each cluster on a flow cell using Sequencing by Synthesis technology and the Genome Analyzer. The improved chemistry of this kit enable researchers to conduct much longer, high-accuracy, sequencing runs than previous versions.

Additional Details

FC-104-4001
ADD TO CART
Genomic DNA Sample Prep Kits

Genomic DNA Sample Prep Kits

Go from purified genomic DNA to a DNA library. Genomic DNA Sample Prep Kits include reagents to build DNA libraries for single-read sequencing. Random size–selected fragments of DNA are created, polished, and prepared for the addition of unique adapters to fragment ends. Following a short PCR enrichment, the library is ready to load.

Additional Details

FC-102-1001
ADD TO CART
Tag Profiling Sample Prep Kits

Tag Profiling Sample Prep Kits

Tag Profiling Sample Prep Kits are used to build constructs comprised of a unique, positionally known 20- or 21-base pair cDNA tag with defined adapters attached to both ends. Access to any mRNA is through two restriction enzyme tag construction options. Kits are available complete or containing only the required oligonucleotides.

Additional Details

FC-102-1005
GET A QUOTE
ChIP-Seq DNA Sample Prep Kit

ChIP-Seq DNA Sample Prep Kit

Chromatin immunoprecipitation (ChIP) selectively finds DNA sequences bound by a particular protein. The ChIP-Seq DNA Sample Prep Kit helps build DNA libraries. The ChIP process enriches specific crosslinked DNA protein complexes using an antibody and unique oligonucleotide adapters added to small stretches of DNA bound to the protein of interest.

Additional Details

IP-102-1001
ADD TO CART
cBot Single Read Cluster Generation Kit

cBot Single Read Cluster Generation Kit

The cBot Single Read Cluster Generation Kit provides reagents that bind DNA library samples to complementary adapter oligonucleotides grafted on the flow cell surface. The pre-mixed, 96-well plate format reagents in this kit provide a streamlined workflow with no reagent preparation and a total of 10 minutes hands-on time.

Additional Details

GD-300-1001
ADD TO CART
cBot Paired-End Cluster Generation Kit

cBot Paired-End Cluster Generation Kit

The cBot Paired-End Cluster Generation Kit provides reagents that bind samples to complementary adapter oligos on the flow cell. These enable copying of the DNA strand after the first sequencing run; the copied strand is used to sequence the other fragment end. The cBot amplifies attached DNA fragments to clonal clusters of ~1000 copies each.

Additional Details

PE-300-1001
ADD TO CART
mRNA-Seq 8-Sample Prep Kit

mRNA-Seq 8-Sample Prep Kit

The mRNA-Seq 8-Sample Prep Kit is used to build DNA libraries for single-read and paired-end sequencing on the Genome Analyzer. Sample preparation is straightforward, using standard molecular biology techniques and requiring minimal hands-on time. mRNA template libraries are sequenced just as any DNA sample using Illumina Sequencing.

Additional Details

RS-100-0801
ADD TO CART
TruSeq RNA Sample Prep Kits

TruSeq RNA Sample Prep Kits

The TruSeq RNA Sample Prep Kits provide a simple, cost-effective solution for generating libraries from total RNA that are compatible with Illumina's unparalleled sequencing output. Master-mixed reagents eliminate the majority of pipetting steps and reduce the amount of clean-up, as compared to previous methods, minimizing hands-on time. New automation-friendly workflow formats enable parallel processing of up to 96 samples. This results in economical, high-throughput RNA sequencing studies achieved with the easiest-to-use sample preparation workflow offered by any NGS platform.

Additional Details

FC-122-1001
ADD TO CART
TruSeq Exome Enrichment Kit

TruSeq Exome Enrichment Kit

TruSeq Exome Enrichment Kit支持了经济高效且可扩展的外显子组测序研究，提供了样品的预富集混合，以及最全面的外显子组覆盖、最高的均一性，和最低的DNA起始量需求。

Additional Details

FC-121-1008
ADD TO CART
TruSeq DNA Sample Prep Kits

TruSeq DNA Sample Prep Kits

TruSeq DNA Sample Prep Kits提供了一个简单、可扩展且经济有效的方案，能从基因组DNA生成文库，并与Illumina无以伦比的测序产量兼容。与之前的方法相比，预混液试剂排除了大部分移液步骤，并减少了纯化次数，让手工操作时间降至最低。全新的自动化友好的流程形式能够平行处理最多96个样品。

Additional Details

FC-121-1001
ADD TO CART
TruSeq SR Cluster Kit v5-CS-GA (single pack)

TruSeq SR Cluster Kit v5-CS-GA (single pack)

TheTruSeq SR Cluster Kit v5-CS-GA (single pack) provides ready-to-load reagents that bind DNA library samples to complementary adapter oligos grafted on the flow cell surface. The master-mixed reagents in this kit provide a streamlined workflow with a total of 10 minutes hands-on time for reagent preparation and setup.

Additional Details

GD-203-5001
ADD TO CART
TruSeq PE Cluster Kit v5-CS-GA (single pack)

TruSeq PE Cluster Kit v5-CS-GA (single pack)

The TruSeq PE Cluster Kit v5-CS-GA provides reagents that bind samples to complementary adapter oligos on the flow cell. These enable copying of the DNA strand after the first sequencing run; the copied strand is used to sequence the other fragment end. The Cluster Station amplifies attached DNA fragments to clonal clusters of ~1000 copies each.

Additional Details

PE-203-5001
ADD TO CART
TruSeq Small RNA Sample Prep Kit

TruSeq Small RNA Sample Prep Kit

TruSeq Small RNA Sample Preparation Kits provide a simple, cost-effective solution for generating small RNA libraries directly from total RNA. These kits enable multiplexed sequencing with the introduction of 48 unique indexes, allowing microRNA discovery and profiling throughput to match Illumina's unparalleled sequencing output.

Additional Details

RS-200-0012
GET A QUOTE
TruSeq SR Cluster Kit v2–cBot -GA

TruSeq SR Cluster Kit v2–cBot -GA

The TruSeq SR Cluster Kit v2–cBot -GA provides reagents that bind DNA library samples to complementary adapter oligonucleotides grafted on the flow cell surface. The pre-mixed, 96-well plate format reagents in this kit provide a streamlined workflow with no reagent preparation and a total of 10 minutes hands-on time.

Additional Details

GD-300-2001
ADD TO CART
TruSeq PE Cluster Kit v2–cBot–GA

TruSeq PE Cluster Kit v2–cBot–GA

The TruSeq PE Cluster Kit v2–cBot-GA provides reagents that bind samples to complementary adapter oligos on the flow cell. These enable copying of the DNA strand after the first sequencing run; the copied strand is used to sequence the other fragment end. The cBot amplifies attached DNA fragments to clonal clusters of ~1000 copies each.

Additional Details

PE-300-2001
ADD TO CART

软件

Illumina为您提供我们引以为荣的系统和分析软件，均为提升您的数据质量和通量水平至最大化而特别设计。每台仪器包括专门设计的系统控制软件以实现最佳性能。数据可用Illumina用户友好的Genome Studio软件或第三方的软件包来分析。

 

 

IDEA Challenge

Illumina has launched a program designed to challenge the scientific community to develop new and creative integrative visualization and data analysis techniques. Learn more about the iDEA Challenge.

文件

文献


视频

支持

Illumina的客户解决方案团队致力于为您提供支持研究所需的资源。即便在您购买第一台仪器之前，您也同样可以获得来自精通Illumina技术、科学应用、软件及硬件系统的科学家的支持。现场服务工程师能熟练地安装您的仪器，并保证它成为一台完全具有功能的系统。现场应用科学家会提供大量的培训，让实验室能快速精通，在无可比拟的通量水平上产生行业领先的数据质量。技术支持科学家通过电话或电子邮件帮助您计划项目，诊断问题，及阐释结果。

我们努力不断超越您的期望，以便您在实验室中将Illumina技术发扬光大。如需了解更多信息，请访问支持部分。


FAQs