Sequencing - 200G to >20g
HiSeq 2500
Genome Analyzer IIx
MiSeq
Sequencing + Arrays
HiScanSQ
Arrays - Low- to High-Plex
HiScan
iScan
BeadXpress
Real-Time PCR System
Eco Real-Time
Whole-Genome DASL HT Assay Kits
| System: | iScan, BeadArray Reader, HiScanSQ |
|---|---|
| Technology: | BeadArray |
| Assay: | Whole-Genome DASL Assay |
| Applications: | FFPE Sample Analysis |
| Species: | Human |
| Contents: | Includes DAP, HumanHT-12 v4 BeadChips, and reagents for amplifying, hybridizing, washing, and processing RNA samples. Available with or without UDG enzyme. |
The Whole-Genome DASL HT Assay is the only integrated system optimized to produce genome-wide expression profiles from low-abundance or partially-degraded human RNA samples, especially those from formalin-fixed, paraffin-embedded (FFPE) tissues. It provides a high-multiplex, low-cost array solution from the simultaneous profiling of over 29,000 transcripts.
The assay combines the unique PCR and labeling steps from Illumina's DASL Assay with gene-based hybridization and the whole-genome probe set of its Direct Hybridization Assay (Human HT-12 v4 Expression BeadChip). This greatly increases the DASL assay target set, which consists of well-characterized NCBI RefSeq genes (RefSeq Build 36.2, Release 38), while retaining the ability to accurately profile partially-degraded RNA samples. Highly reproducible expression profiles (r2 >0.97) can be produced from as little as 10–100 ng total RNA from fresh frozen tissue or 50–200 ng total RNA from FFPE samples.
Specifications
Learn more about how this product is used.
| Contents of Whole-genome DASL HT Assay Kit: | |
|---|---|
| Catalog Number |
DA-905-0024
DA-905-1024 (W/UDG) DA-905-0096
DA-905-1096 (W/UDG) |
| Number of Samples |
24
96
|
| Ship at -65° to -85°C. Store at -15° to -25°C. | |
| MCS3 (cDNA Synthesis Master Mix) |
320µl
680µl
|
| OB1 (Oligo Hybridization and DNA Binding Buffer 1) |
3.5 ml
3.5 ml
|
| MEL (Master Mix for Extension/Ligation Reagent) |
4.0 ml
4.0 ml
|
| IP1 (Inocilate PCR Reagent) |
3.8 ml
3.8 ml
|
| UDG (Uracil DNA Glycosylase*) |
52 µl
52 µl
|
| SCM (Single-Color Master Mix Reagent) |
3.2 ml
3.2 ml
|
| XC4 (XStain BeadChip Solution 4) |
350 ml
350 ml
|
| HCB (Humidity Control Buffer) |
2.8 ml
5.6 ml (2 x 2.8 ml)
|
| HYB (Hybridization Buffer) |
1.7 ml
1.7 ml
|
| Ship at -65° to -85°C. Store at 2° to 8°C. | |
| MPB (Paramagnetic Particles B) |
2.2 ml
72.2 ml
|
| PS1 (Precipitation Solution 1) |
30 ml
30 ml
|
| Ship at 20° to 25°C. Store at 2° to 8°C. | |
| AM1 (Master Mix for Extension/Ligation 1 Reagent) |
15 ml
15 ml
|
| UB1 (Universal Buffer 1) |
25 ml
25 ml
|
| HumanHT-12 v4 BeadChips |
2
8
|
| Ship at 20° to 25°C. Store at 20° to 25°C. | |
| MH1 (Make Hyb1 Reagent) |
7.0 ml (2 x 3.5 ml)
7.0 ml (24 x 3.5 ml)
|
| WBC (Wash E1BC Buffer) |
20.0 ml (4 x 5.0 ml)
20.0 ml (4 x 5.0 ml)
|
| HTW (High-Temperature Wash Buffer) |
325 ml
325 ml
|
| UB2 (Universal Buffer 2) |
300 ml
300 ml
|
| PB1 (Preparing BeadChip for Hybridization Buffer) |
950 ml
950 ml
|
|
*Supplied in DA-903-1024 and DA-903-1096 only. |
|
Applications
FFPE Sample Analysis
Formalin-fixed, paraffin-embedded (FFPE) samples are preserved tissue samples that are generally associated with disease. Many of these samples represent clinical outcomes, which could provide a potential gold mine of information when linked with underlying expression profiles. FFPE samples generally contain partially degraded RNA, so transcription analysis is a challenge for many gene expression assays. By using unique PCR and labeling steps based on the proven GoldenGate chemistry, Illumina's DASL assays provide high-quality data from degraded RNA samples. The DASL assay is available in the Whole-Genome DASL Assay, a fixed content, whole-genome profiling panel.
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