Universal Arrays

System:	iScan, BeadStation, BeadArray Reader, HiScanSQ
Technology:	BeadArray
Assay:	GoldenGate Genotyping Assay
Applications:	Custom Low- to Mid-plex Genotyping
Species:	Any
Contents:	An Array Matrix or BeadChips for processing 96 samples with 96-1536 assays/sample. Requires use of a BeadArray Reader or iScan

There are currently two types of universal BeadArray substrates to choose from. Both make use of the high density self-assembly of oligonucleotide-coated beads, but their unique form factors enable different sample throughput and workflow options.

GoldenGate Assays are deployed on the 12- or 32-sample Universal BeadChips or the 96-sample Universal Array Matrix during the hybridization step of the GoldenGate Genotyping Assay. Instead of gene-specific sequences, they contain universal sequences or addresses that hybridize to complementary sequences in the prepared sample.

• A core feature common to all universal BeadArray substrates is a high level of feature redundancy
• Illumina scientists and engineers have ensured equivalent performance on the Universal Array Matrix and Universal BeadChips

Note that the Universal-12 and -32 BeadChips can be read by the iScan System or BeadArray Reader but the Universal Array Matrix can only be scanned by the BeadArray Reader. Both scanner systems generate reproducible high-quality data due to their high sensitivity, dynamic range, and signal-to-noise ratios.

The principal difference between the two platforms is the significantly higher throughput rate using the iScan System, however performance is analogous when a Universal BeadChip is imaged with either system.