Sequencing - 200G to >20g
HiSeq 2500
Genome Analyzer IIx
MiSeq
Sequencing + Arrays
HiScanSQ
Arrays - Low- to High-Plex
HiScan
iScan
BeadXpress
Real-Time PCR System
Eco Real-Time
TruSeq SR Cluster Kit v5-CS-GA (single pack)
| System: | Genome Analyzer IIx |
|---|---|
| Technology: | Illumina Sequencing |
| Assay: | Single-Read Sequencing |
| Applications: | DNA Sequencing, Gene Regulation Analysis, SNP Discovery and Structural Variation Analysis, Cytogenetic Analysis, Sequencing-Based Methylation Analysis |
| Contents: | Provides kitted reagents for SR cluster generation on the Cluster Station — Supports TruSeq multiplexing on the Genome Analyzer |
The TruSeq SR Cluster Kit v5–CS–GA provides reagents for cluster amplification on single read flow cells. DNA library samples are bound to complementary adapter oligos grafted on the surface of the Illumina Genome Analyzer flow cell. The templates are copied from the hybridized primer by 3’ extension using a high fidelity DNA polymerase to prevent misincorporation errors.
These copies are isothermally amplified to create clonal clusters of ~1,000 copies each, ready for sequencing on the Genome Analyzer IIx. The master-mixed reagents in this kit provide a streamlined workflow with a total of 10 minutes hands-on time for reagent preparation and setup.
Order Information
Required Products
Specifications
Learn more about how this product is used.
| Contents of the TruSeq SR Cluster Kit v5-CS-GA (single pack) | |
|---|---|
| Catalog Number |
GD-103-5001
|
| Box 1 |
|
| Ship on dry ice. Store at -20°C. | |
| HT1 (Hybridization Buffer) |
30 ml
|
| HT2 (Wash Buffer) |
10 ml
|
| HFE (High-Fidelity Extension Mix) |
1.0 ml
|
| AT1 (Formamide) |
9.0 ml
|
| AMX1 (Amplification Mix 1) |
12 ml
|
| LMX1 (Linearization Mix 1) |
1.8 ml
|
| BMX (Blocking Mix) |
2.0 ml
|
| HP3 (2 N NaOH) |
0.2 ml
|
| Other Contents |
|
| Ship on dry ice. Store at -20°C. | |
| Genomic Sequencing Primer |
1
|
| Ship at 2 to 8°C. Store at 2 to 8°C. | |
| Single Read Flow Cell v4 |
1
|
| Ship at RT. Store at RT. | |
| Amp Manifold for Cluster station |
1
|
| Hvb Manifold for Cluster Station |
1
|
Applications
Gene Regulation Analysis
Get a complete view of the epigenome at single-base resolution with the Genome AnalyzerIIx. Whether you are interested in CpG methylation, histone modifications, chromatin structure, or DNA-protein interactions, Illumina's unique sequencing chemistry gives you unbiased coverage of every base in the genome.
More...SNP Discovery and Structural Variation Analysis
Single nucleotide polymorphisms (SNPs) and structural variants are at the root of genetic variation among individuals and populations. This variation influences how individuals differ in their risk of disease and their response to therapeutic treatments. SNPs and structural variants are discovered within the genome by comparing multiple genomic sequences from a diverse sample set of individuals. Illumina's industry-leading sequencing technology provides the sample throughput level and high-quality data required for accurate SNP discovery and structural variation analysis studies.
More...Cytogenetic Analysis
Structural variability is a substantial source of genetic variation that has a major influence on phenotypic variation. Cytogenetic analysis allows researchers to profile chromosomal aberrations such as amplifications, deletions, rearrangements, point mutations, copy number changes, and copy-neutral loss of heterozygosity (LOH) events.
More...Sequencing-Based Methylation Analysis
Identify and track methylation patterns by directly sequencing bisulfite-converted DNA, methylation-sensitive restriction digest-enriched fragments, anti-methyl C-precipitated fragments, or chromatin immunoprecipitates of methyltransferases trapped to aza-labeled DNA on the Genome Analyzer IIx.
More...DNA Sequencing
Illumina’s sequencers powerfully combine the flexibility of single reads, short- and long-insert paired-end reads, enabling the broadest range of genomic applications. TruSeq sequencing chemistry supports this wide range of applications including whole-genome sequencing, targeted resequencing, de novo sequencing, SNP discovery, identification of copy number variations, and chromosomal rearrangement.
More...
