Illumina
TruSeq SR Cluster Kit v3-cBot-HS

TruSeq SR Cluster Kit v3-cbot-HS

System: HiSeq 2000, HiScanSQ, cBot, HiSeq 1000
Technology: Illumina Sequencing
Assay: Single-Read Sequencing, mRNA-Seq, Small RNA Discovery and Analysis, Multiplex Sequencing
Applications: DNA Sequencing, Gene Regulation Analysis, SNP Discovery and Structural Variation Analysis, Cytogenetic Analysis, Sequencing-Based Methylation Analysis
Contents: Provides kitted reagents for cluster generation on 1 single-read flow cell for the HiSeq and HiScanSQ sequencing systems

The TruSeq SR Cluster Kit v3-cBot-HS provides reagents for cluster amplification on single-end flow cells using the cBot cluster amplification system. cBot cluster generation reagents are provided in a pre-mixed, 96-well plate format that requires limited hands-on reagent preparation. When used in combination with TruSeq SBS Kit v3-HS, this kit will enable up to 600 Gb of output per run on the HiSeq2000, or up to 150 Gb of output per run on the HiScanSQ.

Note: This cluster generation kit is used for preparing sequencing templates for HiSeq 2000, HiSeq 1000, and HiScanSQ. It is not compatible with the Genome Analyzer.

DNA library samples are bound to complementary adapter oligos grafted on the surface of the Illumina sequencing flow cell. The templates are copied from the hybridized primer by 3' extension using a high fidelity DNA polymerase to prevent misincorporation errors. These copies are isothermally amplified to create clonal clusters of ~1,000 copies each, ready for sequencing.

Order Information

GD-401-3001
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Specifications

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Contents of the TruSeq SR Cluster Kit v3–cBot–HS
Catalog Number GD-401-3001
HiSeq Cluster Generation Reagent Plate Ship on dry ice. Store at -15° to -25°C.
HT1 (Hybridization Buffer)
HFE (1x PhusionTM Master Mix
(Finnzymes Oy))
APM1 (AMX 1 Premix)
AMX1 (Amplification Mix)
AT1 (100% Formamide)
AMX1 (Amplification Mix)
HT2 (Wash Buffer)
LS1 (Linearization Solution)
BMX (Blocking Mix)
HP5 (0.1 N NaOH)
HP1 (Sequencing Primer)
Single-Read
HiSeq Flow Cell
Ship at RT.
Store at 2°C to 8°C.
Manifold & HiSeq Accessories Kit
Shap at RT. Store at RT.

Applications

Sequencing

Illumina sequencing gives you the power to comprehensively characterize the genome, epigenome or the transcriptome. Go from DNA to data in under a week with less than four hours of hands-on time. Applications flexibility for any genetic variant. Superior raw-read accuracy and the industry's simplest, automated workflow. It's sequencing power for every researcher.

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Gene Regulation Analysis

Get a complete view of the epigenome at single-base resolution with the Genome AnalyzerIIx. Whether you are interested in CpG methylation, histone modifications, chromatin structure, or DNA-protein interactions, Illumina's unique sequencing chemistry gives you unbiased coverage of every base in the genome.

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SNP Discovery and Structural Variation Analysis

Single nucleotide polymorphisms (SNPs) and structural variants are at the root of genetic variation among individuals and populations. This variation influences how individuals differ in their risk of disease and their response to therapeutic treatments. SNPs and structural variants are discovered within the genome by comparing multiple genomic sequences from a diverse sample set of individuals. Illumina's industry-leading sequencing technology provides the sample throughput level and high-quality data required for accurate SNP discovery and structural variation analysis studies.

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Sequencing-Based Methylation Analysis

Identify and track methylation patterns by directly sequencing bisulfite-converted DNA, methylation-sensitive restriction digest-enriched fragments, anti-methyl C-precipitated fragments, or chromatin immunoprecipitates of methyltransferases trapped to aza-labeled DNA on the Genome Analyzer IIx.

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Cytogenetics

Illumina’s cytogenetic analysis solutions give you the ability to rapidly discover and profile structural aberrations across the genome. Our microarray-based assays allow you to screen against all known cytogenetic abnormalities and discover new regions. With next-generation sequencing, combine short-insert and long-insert paired-end reads to catalog any size genomic rearrangement and evaluate complex aberrations.

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