Illumina
TruSeq SBS Kit v3-HS

TruSeq SBS Kit v3-HS

System: HiSeq 2000, HiScanSQ, cBot, HiSeq 1000
Technology: Illumina Sequencing
Assay: Single-Read Sequencing, mRNA-Seq, Mate-Pair Library Sequencing, Multiplex Sequencing, Paired-End Sequencing
Applications: DNA Sequencing, Gene Regulation Analysis, SNP Discovery and Structural Variation Analysis, Cytogenetic Analysis, Sequencing-Based Methylation Analysis
Contents: Two kit sizes are available for either 58 or 209 cycles of sequencing on the HiSeq 2000 and HiScanSQ sequencing systems

TruSeq SBS Kit v3-HS offers pre-configured, ready-to-load reagents that reduce hands-on preparation time to two minutes. When used in conjunction with the TruSeq SR Cluster Kit v3-cBot-HS, this kit enables up to 600 Gb of output per run on the HiSeq 2000 or up to 150 Gb of output per run on the HiScanSQ. When used in conjunction with the TruSeq PE Cluster Kit v3-cBot-HS, it enables up to 150 Gb of output per run on the HiSeq 2000 or up to 75 Gb of output per run on the HiScanSQ .

Sequencing by synthesis (SBS) technology takes advantage of proprietary, fluorescently-labeled, reversibly terminated nucleotides to sequence the hundreds of millions of clusters, base by base, in parallel.

Note: The TruSeq SBS Kit v3-HS is NOT compatible with the Genome Analyzer.

Order Information

FC-401-3001
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Required Products

Applications

DNA Sequencing

Illumina’s sequencers powerfully combine the flexibility of single reads, short- and long-insert paired-end reads, enabling the broadest range of genomic applications. TruSeq sequencing chemistry supports this wide range of applications including whole-genome sequencing, targeted resequencing, de novo sequencing, SNP discovery, identification of copy number variations, and chromosomal rearrangement.

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Gene Regulation Analysis

Get a complete view of the epigenome at single-base resolution with the Genome AnalyzerIIx. Whether you are interested in CpG methylation, histone modifications, chromatin structure, or DNA-protein interactions, Illumina's unique sequencing chemistry gives you unbiased coverage of every base in the genome.

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SNP Discovery and Structural Variation Analysis

Single nucleotide polymorphisms (SNPs) and structural variants are at the root of genetic variation among individuals and populations. This variation influences how individuals differ in their risk of disease and their response to therapeutic treatments. SNPs and structural variants are discovered within the genome by comparing multiple genomic sequences from a diverse sample set of individuals. Illumina's industry-leading sequencing technology provides the sample throughput level and high-quality data required for accurate SNP discovery and structural variation analysis studies.

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Sequencing-Based Methylation Analysis

Identify and track methylation patterns by directly sequencing bisulfite-converted DNA, methylation-sensitive restriction digest-enriched fragments, anti-methyl C-precipitated fragments, or chromatin immunoprecipitates of methyltransferases trapped to aza-labeled DNA on the Genome Analyzer IIx.

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Cytogenetics

Illumina’s cytogenetic analysis solutions give you the ability to rapidly discover and profile structural aberrations across the genome. Our microarray-based assays allow you to screen against all known cytogenetic abnormalities and discover new regions. With next-generation sequencing, combine short-insert and long-insert paired-end reads to catalog any size genomic rearrangement and evaluate complex aberrations.

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Specifications

Contents of the TruSeq SBS Kit v3-HS
Catalog Number FC-401-3001
Box 1
Ship on dry ice.
Store at -15° to -20°C.
CMR (Cleavage Mix Reagent)
SMR (Scanning Mix Reagent)
LRP (Long Read DNA Polymerase)
LFN36 (Long Read Nucleotide Mix)
Box 2
Ship on dry ice.
Store at 2° to 8°C.
PW1 (Wash Solution)
ICR (Incorporation Mix Reagent)
SB1 (High Salt Buffer)
SB2 (Incorporation Buffer)
SB3 (Cleavage Buffer)