Illumina
TruSeq RNA Sample Prep Kit

TruSeq RNA Sample Prep Kits

System: HiSeq 2000, HiScanSQ, Genome Analyzer IIx, HiSeq 1000
Technology: Illumina Sequencing
Assay: mRNA-Seq
Applications: Sequencing-Based Transcriptome Analysis
Contents: Contains reagents for preparing RNA libraries for sequencing

The TruSeq RNA Sample Prep Kits provide a simple, cost-effective solution for generating libraries from total RNA that are compatible with Illumina's unparalleled sequencing output. Master-mixed reagents eliminate the majority of pipetting steps and reduce the amount of clean-up, as compared to previous methods, minimizing hands-on time. New automation-friendly workflow formats enable parallel processing of up to 96 samples at a time. This results in economical, high-throughput RNA sequencing studies achieved with the easiest-to-use sample preparation workflow offered by any NGS platform.

 


Order Information

FC-122-1001
ADD TO CART

Required Products

Eliminating all column purification and gel selection steps from the workflow removes the most time-intensive portions, while improving the assay robustness. It also allows for decreased input levels of RNA-as low as 100 ng- and maintains single copy per gene sensitivity.


TruSeq RNA Sample Prep Workflow


TruSeq RNA Sample Preparation Kit
Catalog Number
Set A: FC-122-1001
Ship at Room Temperature . Store at -15° to -25° C.
RNA Purification Beads (RPB)
1 x 5.0 ml
Ship Frozen (dry ice). Store at 2° to 8°C.
Bead Binding Buffer (BBB)
1 x 5.0 ml
Bead Washing Buffer (BWB)
1 x 60.0 ml
10 mM Tris Buffer pH 7.5 (ELB)
1 x 5.0 ml
Elution, Prime, Fragmentation (EPF)
1 x 2.0 ml
First Strand Master (FSM)
1 x 0.5 ml
Second Strand Master (SSM)
1 x 2.0 ml
Ship Frozen (dry ice). Store at -15° to -25°C.
PCR Master Mix (PMM)
1 x 2.0 ml
PCR Primer Cocktail (PPC)
1 x 0.5 ml
End Repair Mix (ERP)
1 x 5.0 ml
A-Tailing Mix (ATL)
1 x 2.0 ml
Ligation Mix (LIG)
1 x 0.5 ml
End Repair Control (CTE)
1 x 2.0 ml
A-Tailing Control (CTA)
1 x 0.5 ml
Ligase Cotnrol (CTL)
1 x 0.5 ml
Resuspension Buffer (RSB)
1 x 15.0 ml
Stop Ligase Mix (STL)
1 x 2.0 ml
RNA Adapter Index 2 (AR002)
1 x 0.5 ml
RNA Adapter Index 4 (AR004)
1 x 0.5 ml
RNA Adapter Index 5 (AR005)
1 x 0.5 ml
RNA Adapter Index 6 (AR006)
1 x 0.5 ml
RNA Adapter Index 7 (AR007)
1 x 2.0 ml
RNA Adapter Index 12 (AR012)
1 x 0.5 ml

 

TruSeq RNA Sample Preparation Kit
Catalog Number
Set B: FC-122-1002
Ship at Room Temperature. Store at -15° to -25° C.
RNA Purification Beads (RPB)
1 x 5.0 ml
Ship Frozen (dry ice). Store at 2° to 8°C.
Bead Binding Buffer (BBB)
1 x 5.0 ml
Bead Washing Buffer (BWB)
1 x 60.0 ml
10 mM Tris Buffer pH 7.5 (ELB)
1 x 5.0 ml
Elution, Prime, Fragmentation (EPF)
1 x 2.0 ml
First Strand Master (FSM)
1 x 0.5 ml
Second Strand Master (SSM)
1 x 2.0 ml
Ship Frozen (dry ice). Store at -15° to -25°C.
PCR Master Mix (PMM)
1 x 2.0 ml
PCR Primer Cocktail (PPC)
1 x 0.5 ml
End Repair Mix (ERP)
1 x 5.0 ml
A-Tailing Mix (ATL)
1 x 2.0 ml
Ligation Mix (LIG)
1 x 0.5 ml
End Repair Control (CTE)
1 x 2.0 ml
A-Tailing Control (CTA)
1 x 0.5 ml
Ligase Cotnrol (CTL)
1 x 0.5 ml
Resuspension Buffer (RSB)
1 x 15.0 ml
Stop Ligase Mix (STL)
1 x 2.0 ml
RNA Adapter Index 1 (AR001)
1 x 0.5 ml
RNA Adapter Index 3 (AR003)
1 x 0.5 ml
RNA Adapter Index 8 (AR008)
1 x 0.5 ml
RNA Adapter Index 9 (AR009)
1 x 0.5 ml
RNA Adapter Index 10 (AR010)
1 x 2.0 ml
RNA Adapter Index 11 (AR011)
1 x 0.5 ml

Applications

Transcriptome Analysis

RNA sequencing has revolutionized the exploration of gene expression. Advances in the sequencing workflow, from sample preparation through data analysis, enable rapid profiling and deep investigation of the transcriptome. With RNA sequencing, you can characterize all transcriptional activity, coding and non-coding, in any organism without a priori assumptions.

Illumina's unique combination of long and short reads, single and paired-end sequencing, strand specificity, and capacity for tens of millions to billions of reads per run allows you to
  • annotate coding SNPs
  • discover transcript isoforms
  • identify regulatory RNAs
  • characterize splice junctions
  • determine the relative abundance of transcripts

With the greatest daily output available for any sequencing system, transcript profiles can be generated in a single day. RNA sequencing reads can be aligned across splice junctions to identify isoforms, novel transcripts and gene fusions. Identify and quantify both rare and common transcripts, with over six orders of magnitude of dynamic range. Reveal the hidden world of non-coding RNA architecture without prior information.

More...

Small RNA Discovery and Analysis

Small RNA sequencing is a powerful application for Illumina Sequencing, enabling the discovery and profiling of microRNAs and other non-coding RNA on any organism, without prior genome annotation. Using low RNA inputs, you can profile the differential expression of known microRNAs as well as detect novel microRNA targets and wide-ranging sequence variation or "iso-miRs" miRBase accessions. With unprecedented sensitivity and dynamic range, Illumina's industry-leading small RNA sequencing methods allow for the most accurate detection and quantification of rare small RNA sequences.

 

More...