Sequencing - 200G to >20g
HiSeq 2500
Genome Analyzer IIx
MiSeq
Sequencing + Arrays
HiScanSQ
Arrays - Low- to High-Plex
HiScan
iScan
BeadXpress
Real-Time PCR System
Eco Real-Time
TruSeq PE Cluster Kit v5-CS-GA (single pack)
| System: | Genome Analyzer IIx |
|---|---|
| Technology: | Illumina Sequencing |
| Assay: | Paired-End Sequencing |
| Applications: | DNA Sequencing, Gene Regulation Analysis, SNP Discovery and Structural Variation Analysis, Cytogenetic Analysis, Sequencing-Based Methylation Analysis |
| Contents: | Provides kitted reagents for PE cluster generation on the Cluster Station — Supports TruSeq multiplexing on the Genome Analyzer |
The TruSeq PE Cluster Kit v5–CS–GA provides reagents for cluster generation on paired-end flow cells with the Cluster Station. The kit also includes reagents for resynthesis of the reverse strand on the Genome Analyzer IIx. The master-mixed reagents in this kit provide a streamlined workflow with a total of 20 minutes hands-on time for reagent preparation and setup.
DNA library samples are bound to complementary adapter oligos grafted on the surface of the Illumina Genome Analyzer flow cell. The templates are copied from the hybridized primer by 3’ extension using a high fidelity DNA polymerase to prevent misincorporation errors. These are isothermally amplified to create clonal clusters of ~1,000 copies each, ready for sequencing on the Genome Analyzer.
The paired-end flow cell contains proprietary oligos that enable selective cleavage of the forward DNA strand after resynthesis of the reverse strand. The reverse strand is regenerated by bridge amplification with the Paired-End Module (attached to the Genome Analyzer). After resynthesis of the reverse strand, the original forward strand is cleaved and the reverse strand is sequenced for the second read.
Order Information
Required Products
Specifications
Learn more about how this product is used.
| Contents of the TruSeq PE Cluster Kit v5–CS–GA | |
|---|---|
| Catalog Number Box 1 |
PE-203-5001
|
| Ship on dry ice. Store at -20°C. | |
| HT1 (Hybridization Buffer) |
30 ml
|
| HT2 (Wash Buffer) |
10 ml
|
| HFE (High-Fidelity Extension Mix) |
1.0 ml
|
| AT1 (Formamide) |
9.0 µl
|
| AMX1 (amplification Mix 1) |
12 ml
|
| LMX1 (Linearization Mix 1) |
1.8 ml
|
| BMX (Blocking Mix) |
2.0 ml
|
| HP1 (Sequencing Primer Mix 1) |
1.32 ml
|
| HP3 (2 N NaOH) |
0.2 ml
|
| APM1 (AMX1 PreMix) |
10 ml
|
| Box 2 |
GD-103-4001
|
| Ship on dry ice. Store at -20°C. | |
| HT2 (Wash Buffer) |
10 ml
|
| RMX (Resynthesis Mix) |
2.0 ml
|
| AT2 (Formamide) |
5.2 ml
|
| AMX2 (Amplification Mix 2) |
6.0 ml
|
| LMX2 (Linearization Mix 2) |
2.0 ml
|
| BMX (Blocking Mix) |
2.0 ml
|
| HP2 (Sequencing Primer Mix 2) |
1.5 ml
|
| HP3 (2 N NaOH) |
0.2 ml
|
| APM2 (AMX2 Premix) |
6.5 ml
|
| Ship at 2° to 8°C. Store at 2° to 8°C. | |
| Paired-End Flow Cell v4 |
1
|
| Ship at RT. Store at RT. | |
| Amp Manifold for Cluster Station |
1
|
| Hyb Manifold for Cluster Station |
1
|
Applications
Gene Regulation Analysis
Get a complete view of the epigenome at single-base resolution with the Genome AnalyzerIIx. Whether you are interested in CpG methylation, histone modifications, chromatin structure, or DNA-protein interactions, Illumina's unique sequencing chemistry gives you unbiased coverage of every base in the genome.
More...SNP Discovery and Structural Variation Analysis
Single nucleotide polymorphisms (SNPs) and structural variants are at the root of genetic variation among individuals and populations. This variation influences how individuals differ in their risk of disease and their response to therapeutic treatments. SNPs and structural variants are discovered within the genome by comparing multiple genomic sequences from a diverse sample set of individuals. Illumina's industry-leading sequencing technology provides the sample throughput level and high-quality data required for accurate SNP discovery and structural variation analysis studies.
More...Sequencing-Based Methylation Analysis
Identify and track methylation patterns by directly sequencing bisulfite-converted DNA, methylation-sensitive restriction digest-enriched fragments, anti-methyl C-precipitated fragments, or chromatin immunoprecipitates of methyltransferases trapped to aza-labeled DNA on the Genome Analyzer IIx.
More...Cytogenetic Analysis
Structural variability is a substantial source of genetic variation that has a major influence on phenotypic variation. Cytogenetic analysis allows researchers to profile chromosomal aberrations such as amplifications, deletions, rearrangements, point mutations, copy number changes, and copy-neutral loss of heterozygosity (LOH) events.
More...Array-Based Methylation Analysis
DNA methylation plays a critical role in the regulation of gene expression and has been implicated in the etiology of many human diseases including cancer. Methylation profiling with BeadArray technology allows researchers to analyze the effects of aberrant methylation (either hyper- or hypomethylation) for a variety of applications. Illumina has developed a robust methylation profiling platform that provides quantitative methylation measurement at the single-CpG-site level, providing the highest resolution for understanding epigenetic changes.
More...
