Illumina
TruSeq PE Cluster Kit v3-cBot-HS

TruSeq PE Cluster Kit v3-cBot-HS

System: HiSeq 2000, HiScanSQ, cBot, HiSeq 1000
Technology: Illumina Sequencing
Assay: mRNA-Seq, Small RNA Discovery and Analysis, Mate-Pair Library Sequencing, Multiplex Sequencing, Paired-End Sequencing
Applications: DNA Sequencing, Gene Regulation Analysis, SNP Discovery and Structural Variation Analysis, Cytogenetic Analysis, Sequencing-Based Methylation Analysis
Contents: Provides kitted reagents for PE cluster generation on cBot — Supports TruSeq multiplexing on HiSeq platforms

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PE-401-3001
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Specifications

Learn more about how this product is used.

Contents of the TruSeq PE Cluster Kit v3–cBot–HS
Catalog Number PE-401-3001
TruSeq Cluster Generation Reagent Plate Ship on dry ice. Store at -15° to -25°C.
HT1 (Hybridization Buffer)
HFE (1x PhusionTM Master Mix
(Finnzymes Oy))
APM1 (AMX 1 Premix)
AT1 (100% Formamide)
AMX1 (Amplification Mix)
HT2 (Wash Buffer)
LMX1 (Linearization Mix)
BMX (Blocking Mix)
HP5 (0.1 N NaOH)
HP1 (Sequencing Primer)
Read 2 Cluster Resynthesis Kit
Ship on dry ice. Store at -15° to -25°C.
HT2 (Wash Buffer)
HP3 (2 N NaOH)
HP2 (Sequencing Primer Mix 2)
AT2 (100% Formamide)
APM2 (AMX2 Premix)
AMX2 (Amplication Mix 2)
BMX (Blocking Mix)
LMX2 (Linearization Mix 2)
RMX (Resynthesis Mix)
Paired-End
HiSeq Flow Cell
Ship at RT.
Store at 2°C to 8°C..
Manifold & HiSeq Accessories Kit
Shap at RT. Store at RT.

Applications

Sequencing

Illumina sequencing gives you the power to comprehensively characterize the genome, epigenome or the transcriptome. Go from DNA to data in under a week with less than four hours of hands-on time. Applications flexibility for any genetic variant. Superior raw-read accuracy and the industry's simplest, automated workflow. It's sequencing power for every researcher.

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Gene Regulation Analysis

Get a complete view of the epigenome at single-base resolution with the Genome AnalyzerIIx. Whether you are interested in CpG methylation, histone modifications, chromatin structure, or DNA-protein interactions, Illumina's unique sequencing chemistry gives you unbiased coverage of every base in the genome.

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SNP Discovery and Structural Variation Analysis

Single nucleotide polymorphisms (SNPs) and structural variants are at the root of genetic variation among individuals and populations. This variation influences how individuals differ in their risk of disease and their response to therapeutic treatments. SNPs and structural variants are discovered within the genome by comparing multiple genomic sequences from a diverse sample set of individuals. Illumina's industry-leading sequencing technology provides the sample throughput level and high-quality data required for accurate SNP discovery and structural variation analysis studies.

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Sequencing-Based Methylation Analysis

Identify and track methylation patterns by directly sequencing bisulfite-converted DNA, methylation-sensitive restriction digest-enriched fragments, anti-methyl C-precipitated fragments, or chromatin immunoprecipitates of methyltransferases trapped to aza-labeled DNA on the Genome Analyzer IIx.

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Cytogenetics

Illumina’s cytogenetic analysis solutions give you the ability to rapidly discover and profile structural aberrations across the genome. Our microarray-based assays allow you to screen against all known cytogenetic abnormalities and discover new regions. With next-generation sequencing, combine short-insert and long-insert paired-end reads to catalog any size genomic rearrangement and evaluate complex aberrations.

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