Illumina
TruSeq Exome Enrichment Kit

TruSeq Exome Enrichment Kit

System: HiSeq 2000, HiScanSQ, Genome Analyzer IIx, HiSeq 1000
Assay: Single-Read Sequencing, Multiplex Sequencing, Paired-End Sequencing
Applications: DNA Sequencing
Species: Human
Contents: Seven kit sizes are available for 8, 24, 48, 96, 192, 480, and 960 reactions on Illumina sequencing systems. Optimized for use with the TruSeq DNA Sample Prep Kit.

The TruSeq Exome Enrichment Kit supports pre-enrichment sample pooling, offering a cost-effective, scalable exome sequencing solution with the most comprehensive exome coverage, highest uniformity, and lowest DNA input requirement. The TruSeq kit is an in-solution sequence capture method for isolating exonic regions of interest in the human genome using hybrid selection. It enables systematic detection of common and rare variants for high-throughput sequencing on any Illumina sequencer at a lower cost per sample.

With a simple and scalable workflow, Illumina's integrated exome enrichment method offers the most flexible and efficient solution for targeted resequencing. Master-mixed reagents are coupled with plate-based processing for up to 96 samples and volumes are optimized for liquid handling robots, making the process automation-friendly for even higher throughput.

Prior to exome enrichment, libraries are prepared using the TruSeq DNA Sample Prep Kit, which provides a robust indexing solution for each DNA sample, enabling multi-sample pooling of up to six samples in a single enrichment reaction. Multi-sample pooling dramatically reduces hands-on time compared to other available methods, making large high-throughput studies feasible and economical.

Order Information

FC-121-1008
Human
ADD TO CART

Required Products

Specifications

TruSeq Exome Enrichment Kit
Read Length
Number of target genes 20.794
Number of target exons 201,121
Target region size 62 Mb
Number of probes 340,427
% of RefSeq database
covered (Sept 2010)		 91%
% of CCDS database
covered (Sept 2010)		 95%
Recommended library
insert size		 300-400 bp


Catalog number
FC-121-1008
FC-121-1024
FC-121-1048
FC-121-1196
FC-121-1192
FC-121-1480
FC-121-1960
Box 1
Ship at RT. Store at 2° to 8°C
 
 
 
 
 
 
 
Streptavidin Magnetic Beads (SMB)
2 X 5 ml
6 X 5 ml
12 X 5 ml
24 X 5 ml
48 X 5 ml
120 X 5 ml
240 X 5 ml
Wash Solution 1 (WS1)
2 X 5 ml
6 X 5 ml
12 X 5 ml
24 X 5 ml
48 X 5 ml
120 X 5 ml
240 X 5 ml
Wash Solution 3 (WS3)
2 X 5 ml
6 X 5 ml
12 X 5 ml
24 X 5 ml
48 X 5 ml
120 X 5 ml
240 X 5 ml
Neutralization Buffer 2 (ET2)
2 x 0.5 ml
6 x 0.5 ml
12 x 0.5 ml
24 x 0.5 ml
48 x 0.5 ml
120 x 0.5 ml
240 x 0.5 ml
Resuspension Buffer (RSB)
1 x 0.5 ml
3 x 0.5 ml
6 x 0.5 ml
12 x 0.5 ml
24 x 0.5 ml
60 x 0.5 ml
120 x 0.5 ml
 
 
 
 
 
 
 
Box 2
Ship on dry ice. Store at -15° to -25°C
 
 
 
 
 
 
 
Wash Solution 2 (WS2)
2 x 10 ml
6 x 10 ml
12 x 10 ml
24 x 10 ml
48 x 10 ml
120 x 10 ml
240 x 10 ml
Capture Target Buffer (CT1)
2 x 2.0 ml
6 x 2.0 ml
12 x 2.0 ml
24 x 2.0 ml
48 x 2.0 ml
120 x 2.0 ml
240 x 2.0 ml
Elute Target Buffer 1 (ET1)
2 x 2.0 ml
6 x 2.0 ml
12 x 2.0 ml
24 x 2.0 ml
48 x 2.0 ml
120 x 2.0 ml
240 x 2.0 ml
Capture Target Oligos (CTO)
2 x 0.5 ml
6 x 0.5 ml
12 x 0.5 ml
24 x 0.5 ml
48 x 0.5 ml
120 x 0.5 ml
240 x 0.5 ml
2N NaOH (HP3)
2 x 0.5 ml
6 x 0.5 ml
12 x 0.5 ml
24 x 0.5 ml
48 x 0.5 ml
120 x 0.5 ml
240 x 0.5 ml
PCR Master Mix Polymerase (PMM)
1 x 0.5 ml
3 x 0.5 ml
6 x 0.5 ml
12 x 0.5 ml
24 x 0.5 ml
60 x 0.5 ml
120 x 0.5 ml
PCR Primer Cocktail (PPC)
1 x 0.5 ml
3 x 0.5 ml
6 x 0.5 ml
12 x 0.5 ml
24 x 0.5 ml
60 x 0.5 ml
120 x 0.5 ml

Applications

DNA Sequencing

Illumina’s sequencers powerfully combine the flexibility of single reads, short- and long-insert paired-end reads, enabling the broadest range of genomic applications. TruSeq sequencing chemistry supports this wide range of applications including whole-genome sequencing, targeted resequencing, de novo sequencing, SNP discovery, identification of copy number variations, and chromosomal rearrangement.

More...

Cytogenetics

Illumina’s cytogenetic analysis solutions give you the ability to rapidly discover and profile structural aberrations across the genome. Our microarray-based assays allow you to screen against all known cytogenetic abnormalities and discover new regions. With next-generation sequencing, combine short-insert and long-insert paired-end reads to catalog any size genomic rearrangement and evaluate complex aberrations.

More...