Sequencing - 200G to >20g
HiSeq 2500
Genome Analyzer IIx
MiSeq
Sequencing + Arrays
HiScanSQ
Arrays - Low- to High-Plex
HiScan
iScan
BeadXpress
Real-Time PCR System
Eco Real-Time
TruSeq DNA Sample Prep Kits
| System: | HiSeq 2000, HiScanSQ, Genome Analyzer IIx, HiSeq 1000 |
|---|---|
| Assay: | Single-Read Sequencing, Multiplex Sequencing, Paired-End Sequencing |
| Applications: | Sequencing, DNA Sequencing, SNP Discovery and Structural Variation Analysis |
The TruSeq DNA Sample Prep Kits provide a simple, scalable, cost-effective solution for generating libraries from genomic DNA that is compatible with Illumina's unparalleled sequencing output. Master-mixed reagents eliminate the majority of pipetting steps and reduce the amount of clean-up, as compared to previous methods, minimizing hands-on time. New automation-friendly workflow formats enable parallel processing of up to 96 samples. This results in economical, high-throughput DNA sequencing studies achieved with the easiest-to-use sample preparation workflow offered by any sequencing platform. The enhanced adapter design with embedded index sequence offers an improved multiplexing workflow for increased application flexibility. As a part of an integrated sequencing solution, TruSeq DNA Sample Prep kits enable simplicity, convenience, and affordability in library preparation.
- The TruSeq DNA Sample Prep Kit is optimized for use with the TruSeq Exome Enrichment Kit
Order Information
Required Products
The TruSeq DNA Sample Prep Kits are used to prepare DNA libraries with insert sizes from 300–500 bp for single, paired-end, and multiplexed sequencing. The protocol supports shearing by either sonication or nebulization.
| TruSeq DNA Sample Preparation Kit | |
|---|---|
| Catalog Number |
Set A: FC-121-1001
|
| Ship Frozen (dry ice). Store at -15° to -25°C. | |
| PCR Master Mix (PMM) |
1 x 2.0 ml
|
| PCR Primer Cocktail (PPC) |
1 x 0.5 ml
|
| End Repair Mix (ERP) |
1 x 5.0 ml
|
| A-Tailing Mix (ATL) |
1 x 2.0 ml
|
| Ligation Mix (LIG) |
1 x 0.5 ml
|
| End Repair Control (CTE) |
1 x 2.0 ml
|
| A-Tailing Control (CTA) |
1 x 0.5 ml
|
| Ligase Cotnrol (CTL) |
1 x 0.5 ml
|
| Resuspension Buffer (RSB) |
1 x 15.0 ml
|
| Stop Ligase Mix (STL) |
1 x 2.0 ml
|
| DNA Adapter Index 2 (AD002) |
1 x 0.5 ml
|
| DNA Adapter Index 4 (AD004) |
1 x 0.5 ml
|
| DNA Adapter Index 5 (AD005) |
1 x 0.5 ml
|
| DNA Adapter Index 6 (AD006) |
1 x 0.5 ml
|
| DNA Adapter Index 7 (AD007) |
1 x 2.0 ml
|
| DNA Adapter Index 12 (AD012) |
1 x 0.5 ml
|
| TruSeq DNA Sample Preparation Kit | |
|---|---|
| Catalog Number |
Set B: FC-121-1002
|
| Ship Frozen (dry ice). Store at -15° to -25°C. | |
| PCR Master Mix (PMM) |
1 x 2.0 ml
|
| PCR Primer Cocktail (PPC) |
1 x 0.5 ml
|
| End Repair Mix (ERP) |
1 x 5.0 ml
|
| A-Tailing Mix (ATL) |
1 x 2.0 ml
|
| Ligation Mix (LIG) |
1 x 0.5 ml
|
| End Repair Control (CTE) |
1 x 2.0 ml
|
| A-Tailing Control (CTA) |
1 x 0.5 ml
|
| Ligase Cotnrol (CTL) |
1 x 0.5 ml
|
| Resuspension Buffer (RSB) |
1 x 15.0 ml
|
| Stop Ligase Mix (STL) |
1 x 2.0 ml
|
| DNA Adapter Index 1 (AD001) |
1 x 0.5 ml
|
| DNA Adapter Index 3 (AD003) |
1 x 0.5 ml
|
| DNA Adapter Index 8 (AD008) |
1 x 0.5 ml
|
| DNA Adapter Index 9 (AD009) |
1 x 0.5 ml
|
| DNA Adapter Index 10 (AD010) |
1 x 2.0 ml
|
| DNA Adapter Index 11 (AD011) |
1 x 0.5 ml
|
Applications
DNA Sequencing
Illumina’s sequencers powerfully combine the flexibility of single reads, short- and long-insert paired-end reads, enabling the broadest range of genomic applications. TruSeq sequencing chemistry supports this wide range of applications including whole-genome sequencing, targeted resequencing, de novo sequencing, SNP discovery, identification of copy number variations, and chromosomal rearrangement.
More...SNP Discovery and Structural Variation Analysis
Single nucleotide polymorphisms (SNPs) and structural variants are at the root of genetic variation among individuals and populations. This variation influences how individuals differ in their risk of disease and their response to therapeutic treatments. SNPs and structural variants are discovered within the genome by comparing multiple genomic sequences from a diverse sample set of individuals. Illumina's industry-leading sequencing technology provides the sample throughput level and high-quality data required for accurate SNP discovery and structural variation analysis studies.
More...Cytogenetics
Illumina’s cytogenetic analysis solutions give you the ability to rapidly discover and profile structural aberrations across the genome. Our microarray-based assays allow you to screen against all known cytogenetic abnormalities and discover new regions. With next-generation sequencing, combine short-insert and long-insert paired-end reads to catalog any size genomic rearrangement and evaluate complex aberrations.
More...Cancer
Cancer remains one of the leading causes of death worldwide. Increasing costs of healthcare of the escalating prevalence of this disease have spurred the need for innovative discovery and diagnostic approaches. Recent technological advancements in DNA sequencing and microarray-based genetic analysis provide the potential for new discoveries that can lead to a revolution in cancer detection and therapy.
More...
