Sequencing - 200G to >20g
HiSeq 2500
Genome Analyzer IIx
MiSeq
Sequencing + Arrays
HiScanSQ
Arrays - Low- to High-Plex
HiScan
iScan
BeadXpress
Real-Time PCR System
Eco Real-Time
Small RNA Sample Prep Kit
| System: | Genome Analyzer, HiSeq 2000 |
|---|---|
| Technology: | Illumina Sequencing |
| Assay: | Small RNA Discovery and Analysis |
| Applications: | Sequencing-Based Transcriptome Analysis, Small RNA Discovery and Analysis, Array-Based Transcriptome Analysis |
| Contents: | Contains reagents for preparing total 8-100 total RNA samples.... Oligo Only Kit contains only required RNA adapters, RT primer, and PCR Primers for 100 samples for small RNA cDNA construct formation |
With Small RNA Discovery and Analysis, Illumina scientists have designed a sample preparation protocol that enables sequencing of known and novel microRNAs as well as other non-coding RNAs, such as piwi-interacting RNAs and siRNAs. Through this simple workflow, libraries are generated from RNA samples to create templates amenable to cluster generation and sequencing-by-synthesis, the novel pathways at the heart of Illumina Sequencing technology.
Sequencing-based Small RNA Sample Prep Kits offer researchers the flexibility to select the length of the small RNA they wish to investigate, enabling size-focused or broad size range investigation of different classes of small RNA on one universal platform.
The library prep technology is designed to selectively target microRNA, or other poly-cistronic RNAs that have been processed by DICER or similar nucleases, which provide a distinct molecular signature. The RNA fragments are ligated to Illumina adaptors followed by reverse transcription and PCR amplification.
The small RNA libraries are loaded onto the fully automated cBot or Cluster Station where they bind to complementary adapter oligos grafted onto a proprietary flow cell substrate. Generated clusters are sequenced on the Genome Analyzer.
- Sequencing-based Small RNA Discovery and Analysis is a completely open system that enables small RNA discovery of any size in any organism
- Small RNA Discovery and Analysis requires use of Small RNA Sample Prep Kit, Small RNA Cluster Generation Kit, and 36-Cycle Illumina Sequencing Kit
Applications
Transcriptome Analysis
Explore the transcriptome like never before. Characterize all transcriptional activity, coding and non-coding, in any organism without a priori assumptions using the Genome Analyzer and achieve an unprecedented view of the transcriptome.
Illumina's unique combination of long and short reads, strand specificity, and millions of counts allows you to annotate coding SNPs, discover transcript isoforms, identify regulatory RNAs, characterize splice junctions, and determine the relative abundance of transcripts.
Generate transcript profiles in a single day with the greatest daily system output. Align RNA sequencing reads across splice junctions and map to reference genomes. Identify and quantify both rare and common transcripts, with six orders of magnitude of dynamic range. Reveal the hidden world of non-coding RNA architecture without prior information.
More...Small RNA Discovery and Analysis
Small RNA sequencing is a powerful application on the Genome Analyzer, enabling the discovery and profiling of microRNAs and other non-coding RNA on any organism without prior genome annotation. Using low RNA inputs, you can profile the differential expression of known microRNAs as well as detect novel microRNA targets and wide-ranging sequence variation or "iso-miRs" miRBase accessions. With unprecedented sensitivity and dynamic range, Illumina's industry-leading small RNA sequencing methods allow for the most accurate detection and quantification of rare small RNA sequences.
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