Sequencing - 200G to >20g
HiSeq 2500
Genome Analyzer IIx
MiSeq
Sequencing + Arrays
HiScanSQ
Arrays - Low- to High-Plex
HiScan
iScan
BeadXpress
Real-Time PCR System
Eco Real-Time
Mate Pair Library Prep Kit
| System: | Genome Analyzer, HiScanSQ |
|---|---|
| Technology: | Illumina Sequencing |
| Assay: | Mate-Pair Library Sequencing |
| Applications: | DNA Sequencing, SNP Discovery and Structural Variation Analysis, Cytogenetic Analysis |
| Contents: | Kitted reagents for preparing up to 10 Mate Pair libraries |
The Mate Pair Library Prep Kit includes reagents required to generate 400-600 bp Illumina sequencing libraries from 2-5 Kb DNA fragments.
Following DNA fragmentation, 2-5 Kb fragments are end-repaired with biotin labeled dNTPs. The DNA fragments are circularized, and non-circularized DNA is removed by digestion. Circular DNA is fragmented and biotin labels of the fragments (corresponding to the ends of the original DNA ligated together) are affinity purified. Purified fragments are end-repaired and ligated to Illumina Paired-End sequencing adapters.
Additional sequences complementary to the flow cell oligonucleotides are added to the adapter sequence with tailed PCR primers. The final prepared libraries consist of short fragments made up of two DNA segments that were originally separated by several kilobases. These libraries are ready for paired-end cluster generation followed by paired-end sequencing.
- Library generation is complete in less than 3 days but only requires around 6 hours hands-on time
- The Mate Pair Library Prep Kit requires use of a Paired-End Cluster Generation Kit and Illumina Sequencing Kits
Applications
Gene Regulation Analysis
Get a complete view of the epigenome at single-base resolution with the Genome Analyzer. Whether you are interested in CpG methylation, histone modifications, chromatin structure, or DNA-protein interactions, Illumina's unique sequencing chemistry gives you unbiased coverage of every base in the genome.
More...Cytogenetic Analysis
Structural variability is a substantial source of genetic variation that has a major influence on phenotypic variation. Cytogenetic analysis allows researchers to profile chromosomal aberrations such as amplifications, deletions, rearrangements, point mutations, copy number changes, and copy-neutral loss of heterozygosity (LOH) events.
More...Array-Based Methylation Analysis
DNA methylation plays a critical role in the regulation of gene expression and has been implicated in the etiology of many human diseases including cancer. Methylation profiling with BeadArray technology allows researchers to analyze the effects of aberrant methylation (either hyper- or hypomethylation) for a variety of applications. Illumina has developed a robust methylation profiling platform that provides quantitative methylation measurement at the single-CpG-site level, providing the highest resolution for understanding epigenetic changes.
More...Documentation
- Mate Pair Library Sequencing Sample Preparation Guide
- Mate Pair Library v2 Sample Preparation Guide (For 2-5 kb Libraries) (English)
