Infinium HumanMethylation27, RevB BeadChip Kits

The HumanMethylation27 BeadChip provides an efficient solution for surveying genome-wide DNA methylation profiles. Powered by Illumina's revolutionary Infinium Methylation Assay, this BeadChip allows researchers to interrogate 27,578 highly informative CpG sites per sample at single-nucleotide resolution.

The HumanMethylation27 panel targets CpG sites located within the proximal promoter regions of transcription start sites of 14,475 consensus coding sequencing (CCDS) in the NCBI Database (Genome Build 36). In addition, 254 assays cover 110 miRNA promoters. On average, two assays were selected per CCDS gene and from 3-20 CpG sites for >200 cancer-related and imprinted genes.

Unlike other genome-wide methylation arrays, the Infinium HumanMethylation27 BeadChip enables single CpG-site resolution, higher sample throughput (up to 12 samples per array), and requires as little as 500 ng of bisulfite-converted DNA per sample. The Infinium DNA methylation platform is highly suitable for detailing the biological role of DNA methylation in both normal and diseased cells, and for novel DNA methylation marker discovery.

• Methylation profiles can be compared between different samples and results can easily be integrated with RNA expression profiling experiments using GenomeStudio software
• Of all genes represented on the HumanMethylation27 BeadChip, more than 12,700 are also present on the HumanHT-12 Gene Expression BeadChip, permitting the integrated analysis of global methylation status and gene expression levels